Abstract
ß-Glucosidases detected in the leaves and roots of the common beet,Beta vulgaris, have been demonstrated to catalyze hydrolysis of native betacyanins. A method is described for the isolation and purification of ßglucosidase from the roots, which involves ammonium sulfate precipitation, DEAE-cellulose chromatography, and Sephadex gel filtration. Maximum activity of the enzyme is detected at 50°C and pH 8.0; it retains stability within the pH range from 5.1 to 9.2. In leaves, ß-glucosidase is associated with chloroplast membranes; solubilization of the membranes results in enzyme inactivation.
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Zakharova, N.S., Petrova, T.A. ß- Glucosidases from leaves and roots of the common beet,Beta vulgaris . Appl Biochem Microbiol 36, 394–397 (2000). https://doi.org/10.1007/BF02738049
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DOI: https://doi.org/10.1007/BF02738049