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Ochratoxin A: Contamination of grain

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Abstract

Ochratoxin A was quantitatively monitored in grain extracts by indirect solid-phase enzyme immunoassay with the use of an immobilized conjugate of the toxin with gelatin and polyclonal rabbit antibodies raised against the ochratoxin A-BSA conjugate. This monitoring found that 1.7 to 18.5% of the samples were contaminated with the toxin at a concentration of 25.9–291.7 μg/kg. An analysis of forage grain found ochratoxin A at concentrations of 440-3250 μg/kg.

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References

  1. Krogh, P.,Natural Toxins, Eaker, D. and Wadstrom, T., Eds., Oxford: Pergamon, 1980, pp. 673–680.

    Google Scholar 

  2. Word, G.M., Patel, S., Entwisle, A.C., and Boenke, A.,Food Add. Contant., 1996, vol. 13, no. 5, pp. 519–539.

    Google Scholar 

  3. Metody analiza chuzherodnykh veshchestv vpishchevykh produktakh (Methods of Analysis of Foreign Substances in Foods), Podunovoi, L.G., Ed., Moscow: RIATs, 1994.

  4. Nakane, P.K. and Kawaoi, A.,J. Histochem. Cytochem., 1974, vol. 22, no. 2, pp. 1084–1091.

    PubMed  CAS  Google Scholar 

  5. Kononenko, G.P., Burkin, A.A., Zotova, E.V., and Soboleva, N.A.,Prikl. Biokhim. Mikrobiol., 1999, vol. 35, no. 2, pp. 213–218.

    Google Scholar 

  6. Martlbauer, E. and Terplan, G.,Arch. Lebensmittelhyg., 1988, vol. 39, no. 6, pp. 143–147.

    Google Scholar 

  7. Chu, F.S., Chang, F.C., and Hinsdill, R.D.,Appl. Environ. Microbiol., 1976, vol. 31, no. 6, pp. 831–835.

    PubMed  CAS  Google Scholar 

  8. Kawamura, O., Sato, S., Kajii, H., Nagayama, S., Ohtani, K., Chiba, J., and Ueno, Y.,Toxicon, 1989, vol. 27, no. 8, pp. 887–897.

    Article  PubMed  CAS  Google Scholar 

  9. Clarke, J.R., Marquardt, R.R., and Frohlich, A.A.,Food Agric. Immunol., 1995, vol. 7, no. 1, pp. 33–42.

    Article  CAS  Google Scholar 

  10. Chu, F.S.,Arch. Biochem. Biophys., 1971, vol. 147, no. 2, pp. 359–366.

    Article  PubMed  CAS  Google Scholar 

  11. Uchiyama, S. and Saito, Y.,J. Food Hyg. Soc. Jpn., 1987, vol. 28, no. 6, pp. 453–460.

    CAS  Google Scholar 

  12. Hult, K. and Fuch, R.,Mycotoxins and Phycotoxins, Steyn, P.S. and Vleggaar, R., Eds., Amsterdam: Elsevier, 1986, pp. 365–376.

    Google Scholar 

  13. Lee, S.C. and Chu, F.S.,Jpn. Assoc. Anal. Chem., 1984, vol. 67, no. 1, pp. 45–49.

    CAS  Google Scholar 

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Kononenko, G.P., Burkin, A.A., Zotova, E.V. et al. Ochratoxin A: Contamination of grain. Appl Biochem Microbiol 36, 177–180 (2000). https://doi.org/10.1007/BF02737916

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  • DOI: https://doi.org/10.1007/BF02737916

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