Abstract
A laboratory reactor equipped with a screw press was used for the hydrolysis of steam-SO2-exploded willowSalix caprea by a composition ofTrichoderma reesei andAspergillus foetidus enzyme preparations at high substrate concentration. Optimal conditions providing the maximal volume of hydrolysis syrup with maximal sugar concentrations were determined. Two different hydrolysis procedures were developed in order to exclude the initial washing of steam-pretreated plant raw material by large volumes of water, which was necessary to eliminate the inhibitory effect of explosion byproducts on enzymatic hydrolysis. The first procedure included enzymatic prehydrolysis of the substrate for 1 h; separation of sugar syrup containing 40–60 g/l glucose, 20–25 g/l xylose, and up to 10 g/l disaccharides, as well as up to 35% of the initial enzymatic activity; then addition of a diluted acetate buffer (pH 4.5); and subsequent hydrolysis of the substrate by the adsorbed enzymes leading to the final accumulation of up to 140 g/l glucose and up to 15 g/l of xylose. In the second scenario, the exploded willow was initially adjusted by alkali to pH 4.5 and then hydrolyzed directly by the added enzymes over 24 h. This procedure resulted in a nearly total polysaccharide hydrolysis and accumulation of up to 170 g/l glucose and 20 g/l xylose. The reasons for inhibition of enzymatic hydrolysis are discussed.
Similar content being viewed by others
References
Szengyel, Z., Zacchi, G., and Reczey, K.,Appl. Biochem. Biotechnol., 1997, vols. 63–65, pp. 351–362.
Saddler, J.N. and Gregg, D.J., Biomass for Energy and the Environment,Proc. 9th European Bioenergy Conference, Chartier, P., Ferrero, G.L., Henius, U.M., Hultberg, S., Sachau, J., and Wiinblad, M., Eds., Oxford, N.Y., Tokyo: Pergamon, 1996, vol. 1. pp. 349–355.
Sinitsyn, A.P., Clesceri, L.S., and Bungay, H.R.,Appl. Biochem. Biotechnol., 1982, vol. 7, pp. 455–458.
Mes-Hartree, M. and Saddler, J.N.,Biotechnol. Lett., 1983, vol. 5, pp. 531–536.
Dekker, R.F.H.,Appl. Microbiol. Biotechnol., 1988, vol. 29, pp. 593–598.
Palmquist, E., Hahn-Hagerdal, B., Galbe, M., Larsson, M., Stenberg, K., Szengyel, Z., Tenborg, S., and Zacchi, G.,Bioresour. Technol., 1997, vol. 58, no. 2, pp. 171–179.
von Sivers, M., Zacchi, G., Olsson, L., Hahn-Hagerdal, B.,Biotechnol. Prog., 1994, vol. 10, pp. 555–560.
Larsson, M. and Zacchi, G.,Bioprocess. Eng., 1996, vol. 15, pp. 125–132.
Mandels, M., Andreotti, R., and Roche, C.,Biotechnol. Bioeng. Symp., 1976, vol. 6. pp. 21–32.
Rabinovich, M.L., Klesov, A.A., and Berezin, I.V.,Dokl. Akad. Nauk SSSR, 1984, vol. 274, pp. 758–763.
Rabinovich, M.L., Melnick M.S., Badalov, A.B., Klesov, A.A., and Berezin, I.V.,Dokl. Akad. Nauk SSSR, 1985, vol. 282, pp. 1013–1017.
Jones, E.O. and Lee, J.M.,Biotechnol. Bioeng., 1988, vol. 31, pp. 35–40.
Deeble, M.F. and Lee, J.M.,Biotechnol. Bioeng. Symp., 1985, vol. 15, pp. 227–235.
Esterbauer, H., Symp. on the Pretreatment of Lignocellulosic Materials,Preprint of Forest Res. Inst., Rotorua, NZ, 1985, Session 2, pp. 1–16.
Toon, S.T., Philippidis, G.P., Ho, N.W.Y., Chen, Z.D., Brainard, A., Lumpkin, R.E., and Riley, C.,J. Appl. Biochem. Biotechnol., 1997, vols. 63–65, pp. 243–255.
Author information
Authors and Affiliations
Additional information
Deceased.
Rights and permissions
About this article
Cite this article
Pristavka, A., Kodituvakky, P.A., Kozlov, Y.P. et al. High-solids enzymatic hydrolysis of steam-exploded willow without prior water washing. Appl Biochem Microbiol 36, 101–108 (2000). https://doi.org/10.1007/BF02737902
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02737902