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Immediate-early gene expression in the brain of the thiamine-deficient rat

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Abstract

Pyrithiamine-induced thiamine deficiency (PTD) in the rat is associated with neuronal loss in the thalamus and inferior colliculus. Recently, we were able to demonstrate the occurrence of apoptosis in the thalamus of these animals. Given that immediate-early genes (IEGs) participate in signal transduction pathways that mediate programmed cell death, the present study utilizedin situ hybridization and immunohistochemistry to examine the expression of four IEGs (c-fos, c-jun,fos-B, and NGFI-A) during the progression of PTD. Elevated c-fos mRNA levels were initially observed in the posterior medial thalamus on d 12 of the deficiency. At the acute symptomatic stage (characterized by a loss of righting reflex on d 16–17), the posterior-medial thalamus exhibited increased mRNA for all genes examined, whereas the inferior colliculus demonstrated mRNA induction for c-fos, c-jun, and NGFI-A. Immunohistochemical analysis revealed that elevations of IEG mRNA associated with the acute symptomatic stage were consistently translated into protein in the thalamus. In contrast, whereas elevated Fos- and Jun-like immunoreactivity were detected in the inferior colliculus at this stage, NGFI-A-like immunoreactivity declined significantly below basal levels, suggesting a translational block. These results are consistent with our recent findings of apoptotic cell death, and indicate that differential patterns of IEG expression occur in the thalamus and inferior colliculus during PTD, which may contribute to the pathogenesis of this disorder.

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Authors and Affiliations

  1. Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec

    Alan S. Hazell

  2. Department of Pharmacology, University of Ottawa, Ottawa, Ontario, Canada

    Lynda McGahan & George S. Robertson

  3. Department of Physiology, University of Ottawa, Ottawa, Ontario, Canada

    Wolfram Tetzlaff & Annie M. Bedard

  4. Neuroscience Research Institute, University of Ottawa, Ottawa, Ontario, Canada

    Lynda McGahan & Antoine M. Hakim

  5. Department of Biochemistry, Medical Institute of Bioregulation, Kuyshu University 69, 812, Fukuoka, Japan

    Yusaku Nakabeppu

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  1. Alan S. Hazell
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  2. Lynda McGahan
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  4. Annie M. Bedard
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  5. George S. Robertson
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  6. Yusaku Nakabeppu
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  7. Antoine M. Hakim
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Hazell, A.S., McGahan, L., Tetzlaff, W. et al. Immediate-early gene expression in the brain of the thiamine-deficient rat. J Mol Neurosci 10, 1–15 (1998). https://doi.org/10.1007/BF02737081

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