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Mg2+- ATPase in the fish brain and its ultrastructural localization

  • Morphological Bases for Evolution of Functions
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Abstract

Effects of γ-aminobutyric acid (GABA) and ethanol on Mg2+-ATPase from mitochondrial and microsomal fractions of the fish brain were studied. GABA (10-8–10-4 M) activates microsomal Mg2+-ATPase, but has no effect on the mitochondrial enzyme activity. This effect of GABA on the microsomal Mg2+-ATPase was absent in the presence of 8% ethanol. Ethanol at 1– 10% concentrations inhibits the basal microsomal Mg2+-ATPase and has no effect on the mitochondria enzyme. Using cytochemical technique, Mg2+-ATPase was revealed both in neurons and in glial cells. The ethanol-sensitive Mg2+-ATPase is located in the area of synaptic junctions and is bound to plasma, vesicular, and smooth endoplasmic reticulum membranes.

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Authors and Affiliations

  1. Papanin Institute of Internal Waters Biology, Russian Academy of Sciences, Borok, Yaroslavl oblast, Nekouz region, Russia

    S. A. Menzikov, N. N. Ruzhinskaya & O. V. Menzikova

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  1. S. A. Menzikov
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  2. N. N. Ruzhinskaya
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  3. O. V. Menzikova
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Menzikov, S.A., Ruzhinskaya, N.N. & Menzikova, O.V. Mg2+- ATPase in the fish brain and its ultrastructural localization. J Evol Biochem Phys 36, 346–352 (2000). https://doi.org/10.1007/BF02737052

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  • DOI: https://doi.org/10.1007/BF02737052

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