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N-Methyl-D-Aspartate receptors in acquisition of the short-term memory in the honeybeeApis mellifera

  • Comparative and Ontogenic Physiology
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Abstract

Role of NMDA receptors in the process of associative learning has been studied in the honeybeeApis mellifera L. in behavior experiments, using method of conditional reflexes. To determine pharmacological profile of NMDA receptors, effects of Mg2+ ions, NMDA, glycine (Gly), antagonist of the glycine site 5,7-dichlorokynurenic acid (DCK), competitive antagonists of NMDA receptors: D,L-2-aminophosphovalerate (APV), L-2-aminophosphobutyrate (APB), and D-glutamyl-aminomethylphosphonic acid (GAMP) as well of antagonists of NMDA receptor ion channels MK-801 and ketamine (Ket) were studied on acquisition of alimentary conditional reflex and its retention in memory. NMDA increased capacity for learning by stimulating shortterm memory. The NMDA receptor co-agonist Gly activated this NMDA effect. DCK eliminated the NMDA and Gly stimulatoty effects. All tested antagonists at millimolar concentrations inhibited associative function. The data obtained confirm our hypothesis about participation of NMDA receptors in processes of formation of short-term memory in the honeybeeApis mellifera and suggest that functional characteristics of the NMDA receptors involved in the process of associative learning in the honeybee resemble those in mammals.

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Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia

    N. G. Lopatina, I. V. Ryzhova, E. G. Chesnokova & L. A. Dmitrieva

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  1. N. G. Lopatina
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  2. I. V. Ryzhova
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  3. E. G. Chesnokova
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  4. L. A. Dmitrieva
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Lopatina, N.G., Ryzhova, I.V., Chesnokova, E.G. et al. N-Methyl-D-Aspartate receptors in acquisition of the short-term memory in the honeybeeApis mellifera . J Evol Biochem Phys 36, 294–300 (2000). https://doi.org/10.1007/BF02737046

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  • DOI: https://doi.org/10.1007/BF02737046

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