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Transgenic potato plants expressing oxalate oxidase have increased resistance to oomycete and bacterial pathogens

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Summary

Potato (cv. Bintje) was transformed with a gene encoding an oxalate oxidase from wheat under the control of the CaMV35S promoter. Transgenic potato plants produced high constitutive levels of H2O2 as visualized by 4-chloro-l-naphtol staining. The resistance of these plants was tested againstPhytophthora infestans. An increased level of resistance to the disease was marked by a reduced number of lesions as well as by a decreased number of sporangia formed per lesion. In addition, oxalate oxidase overexpressing plants also exhibited improved resistance toStreptomyces reticuliscabiei, the causal agent of netted scab. Increased expression of oxalate oxidase had no effect on the interaction withErwinia carotovora. These experiments show that overexpression of oxalate oxidase represents a potentially interesting approach for protection of potato to pathogens.

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Author notes

  1. M. Schneider

    Present address: Swiss Institute of Bioinformatics, Swiss-Prot, CMU, 1 Michel-Servet, CH 1211, Geneva 4, Switzerland

Authors and Affiliations

  1. Département de Biologie-Biologie végétale, Université de Fribourg, ch. du musée 10, CH 1700, Fribourg, Switzerland

    M. Schneider & J. -P. Métraux

  2. Station fédérale de Changins (RAC), Route de Duillier, Case postale 254, CH-1260, Nyon, Switzerland

    E. Droz & Pia Malnoë

  3. Germicopa, allée Loeiz Herrieu, F-29334, Quimper Cedex, France

    Catherine Chatot & E. Bonnel

Authors
  1. M. Schneider
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Schneider, M., Droz, E., Malnoë, P. et al. Transgenic potato plants expressing oxalate oxidase have increased resistance to oomycete and bacterial pathogens. Potato Res 45, 177–185 (2002). https://doi.org/10.1007/BF02736113

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