Summary
Four methods of assessment of tuber blight resistance were compared. On the day of harvest in August undamaged tubers were inoculated and incubated in the laboratory in controlled conditions (method a) or incubated first for seven days in a clamp (b) or tubers from storage were tested in January after damage (c) or in November by tuber slice test (d). Over three years, 41 varieties were examined and on average, differences of 2.2 to 3.2 score on a 1—9 scale, showed significant differences between cultivars. The interactions varieties x years, varieties x methods and years x methods were significant in an analysis of variance over methods a, c, d together. Method-specific differences are explained as tissue-specific resistance of some cultivars. Tissue-specifity of components of resistance require to use a whole tuber test (method a) in case of low tuber injuries during harvest in practice. The resistance of the methods correlated with each other r=0.2—0.5. The state of skin, number and size of lenticels did not correlate with the resistance. Tuber blight resistance in the German descriptive list of cultivars was over-rated by about 2 scores.
Similar content being viewed by others
References
Anonymous, 1992. Beschreibende Sortenliste für Kartoffeln. Bundessortenamt, Deutschland. Boyd, A.E.W., 1980. Development of potato blight(Phytophthora infestans) after planting infected seed tubers.Annals of Applied Biology 95: 301–309.
Boyd, A.E.W. & J.M. Henderson, 1953. Susceptibility of immature potato tubers to blight.Plant Pathology 2: 113–116.
Darsow, U., 1983. Prüfung unverletzter erntefrischer Kartoffelknollen auf relative Braunfäuleresistenz.Archiv für Züchtungsforschung 13: 357–366.
Darsow, U., 1987. Long term results of a tuber slice test for relative resistance to late blight.Potato Research 30: 9–22.
Darsow, U., 1988. Prädisponierende Wirkung des Ernte- und Prüfungstermins sowie des Keimzustandes der Kartoffelknollen auf die relative Braunfäuleresistenz im Scheibentest.Archiv für Phytopathologie und Pflanzenschutz 24: 203–212.
Darsow, U., 1992. Effekt und Risiko des Knöllchentests auf Braunfäuleresistenz bei Kartoffelsämlingen im System der Auslese in derPhytophthora-Resistenzzüchtung.Journal of Phytopathology 135: 199–206.
Darsow, U., 2000. 50 Jahre Züchtungsforschung zuPhytophthora infestons bei Kartoffeln in Groß Lüsewitz. Geschichte einer Resistenzzüchtung mit Wechsel von der vertikalen zur horizontalen Resistenz.Beiträge zur Züchtungsforschung 6: 1–49.
Darsow, U. & G. Meinl, 1981. Untersuchungen zum Befall wachsender sowie unterschiedlich verletzter Kartoffelknollen mitPhytophthora infestons (Mont.) de Bary.Archiv für Züchtungsforschung 11: 55–66.
Deahl, K.L., M.E. Gallegly & R.J. Young, 1974. Laboratory testing of potato tubers for multigenic resistance to late blight.American Potato Journal 51: 324–329.
Dorrance, A.E. & D.A. Inglis, 1998. Assessment of laboratory methods for evaluating potato tubers for resistance to late blight.Plant Disease 82: 442–446.
Dowley, L.J. & E. O’Sullivan, 1991. Sporulation ofPhytophthora infestons (Mont.) de Bary on the surface of diseased potatoes and tuber to tuber infection during handling.Potato Research 34: 295–296.
Durska, B., 1975. Wykorzystanie roznych method w okreslaniu odpornosci bulw na zaraze ziemniaka (Phytophthora infestans (Mont.) de Bary) na podstawie badau odmian ziemniaka zrejonizowanych w Polsce.Biuletyn Instytutu Ziemniaka 16: 19–39.
Friend, J., 1991. Host-pathogen interactions: current questions. In: J.A. Lucas, R.C. Shattock, D.S. Shaw & L.R. Cooke (Eds),Phytophthora. Cambridge University Press, Cambridge, pp. 46–49.
Howard, H.W., 1965. Testing for resistance of potato tubers to infection withPhytophthora infestans.European Potato Journal 8: 184.
Kirk, W.W., B.A. Niemira & J.M. Stein, 2001. Influence of storage temperature on rate of potato tuber tissue infection caused byPhytophthora infestans (Mont.) de Bary estimated by digital image analysis.Potato Research 44: 86–96.
Landeo, J.A., M. Gastelo, H. Pinedo & F. Flores, 1995. Breeding for horizontal resistance to late blight in potato free of R-genes. In: L.J. Dowley, E. Bannon, L.R. Cooke, T. Keane & E. O’Sullivan (Eds),Phytophthora infestans 150. Boole Press, Dublin, pp. 268–274.
Lapwood, D.H., 1967. Laboratory assessments of the susceptibility of potato tubers to infection by blight(Phytophthora infestans).European Potato Journal 10: 127–135.
Lapwood, D.H., 1977. Factors affecting the field infection of potato tubers of different cultivars by blight(Phytophthora infestans).Annals of Applied Biology 85: 23–42.
Lapwood, D.H. & R.K. McKee, 1961. Reaction of tubers of R-gene potato clones to inoculation with specialized races of P.infestans.European Potato Journal 4: 3–13.
Monson, A.M. & C.J. Eide, 1964. Evaluation of the resistance of potato tubers to penetration and invasion byPhytophthora infestans.American Potato Journal 41: 300.
Muskens, M.M.W.M. & S.J.J.H.M. Allefs, 2002. Breeding for late blight resistance, views from practice. Abstracts 15th triennial conference EAPR, Hamburg, pp. 85.
Parker, J.E., W. Knogge & D. Scheel, 1991. Molecular aspects of host-pathogen interactions inPhytophthora. In: J.A. Lucas, R.C. Shattock, D.S. Shaw & L.R. Cooke (Eds),Phytophthora. Cambridge University Press, Cambridge, pp. 90–103.
Pathak, N. & D.D. Clarke, 1987. Studies on the resistance of the outer cortical tissues of the tubers of some potato cultivars toPhytophthora infestons.Physiological and Molecular Plant Pathology 31: 123–132.
Pietkiewicz, J.B. & G.J. Jellis, 1976. Testing potato tubers for resistance to blight(Phytophthora infestons (Mont.) de Bary) in the laboratory.Ziemniak 37: 129–135.
Ross, H., 1986. Potato breeding — Problems and Perspectives.Journal of Plant Breeding, Supplement 13, 132 p.
Schöber, B., 1987. Beurteilung und Erfassung von Braunfäule(Phytophthora infestons (Mont.) de Bary) in den Knollen. In: E. Førsund (Ed.), Potato Disease Assessment Keys, EAPR, Wageningen, pp. 28–35.
Schöber, B. & E. Höppner, 1972. Zur Methodik der Resistenzprüfung von Kartoffelknollen gegen den Erreger der Braunfäule,Phytophthora infestons (Mont.) de Bary.Potato Research 15: 378–383.
Schöber, B. & E. Schiessendoppler, 1983. Vergleichende Resistenzprüfung von Kartoffelknollen gegen den Erreger der Braunfäule,Phytophthora infestons (Mont.) de Bary.Potato Research 26: 179–181.
Singh, B.P., T.A. Joseph & S.K. Kaushik, 1999. Breeding for disease resistance. In: B.P. Singh & G.S. Shekhawat (Eds), Potato late blight in India. Technical Bulletin 27, Malhotra Publication House 1999, New Delhi, pp. 55–69.
Song, J., J.M. Bradeen, S.K. Naess, J.A. Raasch, S.M. Wielgius, G.T. Haberlach, J. Liu, H. Kuang, S. Austin-Phillips, C.R. Buell, J.P. Helgeson & J. Jiang, 2003. Gene RB cloned fromSolanum bulbocastanum confers broad spectrum resistance to late blight.Proceedings National Academy of Science USA 100: 9128–9133.
Stephan, S., 1970. Untersuchungen zum Auftreten der Braunfäule an Kartoffeln auf der Grundlage von Beobachtungen des Staatlichen Pflanzenschutzdienstes.Nachrichtenblatt für den Deutschen Pflanzenschutzdienst Berlin 24: 41–47.
Stewart, H.E., D.C. McCalmont & R.L. Wastie, 1983. The effect of harvest date and the interval between harvest and inoculation on the assessment of the resistance of potato tubers to late blight.Potato Research 26: 101–107.
Swiezynski, K.M., L. Domanski, M. Osiecka, M.T. Siecka & H. Zarzycka, 1977. Resistance toPhytophthora infestons in diploid and tetraploid potato families. 2. Resistance in tubers.Journal of Applied Genetics 38: 33–44.
Swiezynski, K.M. & E. Zimnoch-Guzowska, 2001. Breeding potato cultivars with tubers resistant toPhytophthora infestons.Potato Research 44: 97–117.
Toxopeus, H.J., 1958. Some notes on the relations between field resistance toPhytophthora infestons in leaves and tubers and ripening time inSolanum tuberosum ssp.tuberosum.Euphytica 7: 123–130.
Walmsley-Woodward, D.J. & B.G. Lewis, 1977. Laboratory studies of potato tuber resistance to infection byPhytophthora infestans..Annals of Applied Biology 85: 43–49.
Wastie, R.L., 1991. Breeding for resistance. In: D.S. Ingram & P.H. Williams (Eds),Phytophthora infestans, the cause of late blight of potato.Advances in Plant Pathology 7: 193–224.
Weber, E., 1967. Grundriss der biologischen Statistik. 6. Auflage. Fischer-Verlag, Jena, 674 S.
Zaag, D.E. van der, 1959. Some observations on breeding for resistance toPhytophthora infestans.European Potato Journal 2: 278–286.
Zimnoch-Guzowska, E. & B. Flis, 2002. Resistance evaluation ofPhytophthora infestans in potato. GILB ’02 Conference Late blight: Managing the global treat, Hamburg. Paper.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Darsow, U. The use of four different assessment methods to establish relative potato tuber blight resistance for breeding. Potato Res. 47, 163–174 (2004). https://doi.org/10.1007/BF02735982
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02735982