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Discontinuous electrophoresis of glycosaminoglycans: A screening method for mucopolysaccharidoses

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Abstract

A modified discontinuous electrophoretic method for the separation of standard and urinary glycosaminoglycans has been reported. The merits of the method are the simple and easy to handle apparatus, non-requirement of elaborate cooling system, sensitivity and high reproducibility of the results and applicability of the method for the preliminary grouping of the MPS patients to reduce the number of enzyme assays to be done.

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Abbreviations

MPS:

Mucopolysaccharidoses

GAGs:

Glycosaminoglycans

DS:

Dermatan sulfate

SDS:

Slow moving dermatan sulfate

FDS1:

Fast moving dermatan sulfate 1

FDS2:

Fast moving dermatan sulfate 2

HS:

Heparan sulfate

KS:

Keratan sulfate

C6S:

Chondroitin-6-sulfate

C4S:

Chondroitin-4-sulfate

CS:

Chondroitin sulfates

CPC:

Cetylpyridinium chloride

CAME:

Cellulose acetate membrane electrophoresis.

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Authors and Affiliations

  1. Department of Genetics, Dr. A.L. Mudaliar Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, 600 113, Madras, India

    E. M. Elango, S. Priya & R. Mayasundari

Authors
  1. E. M. Elango
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  2. S. Priya
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  3. R. Mayasundari
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Elango, E.M., Priya, S. & Mayasundari, R. Discontinuous electrophoresis of glycosaminoglycans: A screening method for mucopolysaccharidoses. Indian J Pediatr 65, 597–601 (1998). https://doi.org/10.1007/BF02730903

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