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Modulation of allele leakiness and adaptive mutability inEscherichia coli

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Abstract

It is shown that partial phenotypic suppression of two ochre mutations (argE3 andlacZU118) and an amber mutation (inargE) by sublethal concentrations of streptomycin in anrpsL + (streptomycin-sensitive) derivative of theEscherichia coli strain AB1157 greatly enhances their adaptive mutability under selection. Streptomycin also increases adaptive mutability brought about by theppm mutation described earlier. Inactivation ofrecA affects neither phenotypic suppression by streptomycin nor replication-associated mutagenesis but abolishes adaptive mutagenesis. These results indicate a causal relationship between allele leakiness and adaptive mutability.

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References

  • Benson S. 1997 Adaptive mutation: a general phenomenon or a special case.BioEssays 19, 9–11.

    Article  PubMed  CAS  Google Scholar 

  • Bochner B., Huang H. C., Schieva G. and Ames B. N. 1980 Positive selections for loss of tetracycline resistance.J. Bacteriol. 143, 926–933.

    PubMed  CAS  Google Scholar 

  • Bridges B. A. 1998 The role of DNA damage in stationary phase (adaptive) mutation.Mutat. Res. 408, 1–9.

    PubMed  CAS  Google Scholar 

  • Bridges B. A. and Eriera S. 1998 DNA synthesis and viability of amutT derivative ofEscherichia coli WP2 under conditions of amino acid starvation and relation to stationary phase (adaptive) mutation.J. Bacteriol. 180, 2906–2910.

    PubMed  CAS  Google Scholar 

  • Cairns J. 1998 Mutation and cancer: the antecedents to our studies of adaptive mutation.Genetics 148, 1433–1440.

    PubMed  CAS  Google Scholar 

  • Foster P. L. 1993 Adaptive mutation: the uses of adversity.Annu. Rev. Microbiol. 47, 467–504.

    Article  PubMed  CAS  Google Scholar 

  • Foster P. L. 1998 Adaptive mutation: Has the unicorn landed?Genetics 148, 1453–1459.

    PubMed  CAS  Google Scholar 

  • Gale E. F., Cundliffe E., Reynolds P. E., Richmond M. H. and Warring M. J. 1972The molecular basis of antibiotic action. Wiley, London.

    Google Scholar 

  • Galitsky T. and Roth J. R. 1996 A search for a general phenomenon of adaptive mutability.Genetics 143, 645–659.

    Google Scholar 

  • Gorini L. and Kataja E. 1965 Suppression activated by streptomycin and related antibiotics inE. coli.Biochem. Biophys. Res. Commun. 18, 656–663.

    Article  CAS  Google Scholar 

  • Hall B. G. 1990 Spontaneous point mutations that occur more often when advantageous than when neutral.Genetics 126, 5–16.

    PubMed  CAS  Google Scholar 

  • Jayaraman R. 1995 Leakiness of genetic markers and susceptibility to postplating mutagenesis inEscherichia coli.J. Genet. 74, 85–97.

    Article  CAS  Google Scholar 

  • Jayaraman R. 1999 Emergence of a mutagenic ochre suppressor mutation under lactose selection ina ppm mutant ofEscherichia coli harbouring theF’lacZU118 episome.J. Genet. 78, 35–42.

    Article  CAS  Google Scholar 

  • Kaji H. and Kaji A. 1965 Specific binding of tRNA to ribosomes: effect of streptomycin.Proc. Natl. Acad. Sci. USA 54, 213–218.

    Article  PubMed  CAS  Google Scholar 

  • Lenski R. E. and Mittler J. E. 1993 The directed mutation controversy and neo-Darwinism.Science 259, 188–194.

    Article  PubMed  CAS  Google Scholar 

  • Lewin B. 1974Gene expression, vol. I. Wiley, London.

    Google Scholar 

  • MacPhee D. G. 1993 Is there evidence of directed mutation in bacteria?Mutagenesis 8, 3–5.

    Article  PubMed  CAS  Google Scholar 

  • Meinnel T., Schmitt E., Mechalan Y. and Blanquet S. 1992 Structural and biochemical characterization of theEscherichia coli argE3 gene product.J. Bacteriol. 174, 2323–2331.

    PubMed  CAS  Google Scholar 

  • Pestka S., Marshall R. and Nirenberg M. 1965 RNA codewords and protein synthesis V. Effect of streptomycin on the formation of ribosome-tRNA complexes.Proc. Natl. Acad. Sci. USA 53, 639–646.

    Article  PubMed  CAS  Google Scholar 

  • Rosche W. A., Foster P. L. and Cairns J. 1999 The role of transient hypermutators in adaptive mutation inEscherichia coli.Proc. Natl. Acad. Sci. USA 96, 6862–6867.

    Article  PubMed  CAS  Google Scholar 

  • Rosenberg S. M., Thulin C. and Harris R. S. 1998 Transient and heritable mutators in adaptive mutation in the lab and in nature.Genetics 148, 1559–1566.

    PubMed  CAS  Google Scholar 

  • Sniegowski P. D. 1995 The origin of adaptive mutants: random or non random?J. Mol. Evol. 40, 94–101.

    Article  CAS  Google Scholar 

  • Strigini P. and Gorini L. 1970 Ribosomal mutations affecting efficiency of amber suppression.J. Mol. Biol. 47, 517–530.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Biological Sciences, Madurai Kamaraj University, 625021, Madurai, India

    R. Jayaraman

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  1. R. Jayaraman
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Correspondence to R. Jayaraman.

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Jayaraman, R. Modulation of allele leakiness and adaptive mutability inEscherichia coli . J Genet 79, 55–60 (2000). https://doi.org/10.1007/BF02728946

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  • DOI: https://doi.org/10.1007/BF02728946

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