Abstract
InNeurospora crassa,0.44 mM Be2+ caused half-maximal inhibition of growth and this inhibition could be fully counteracted by the addition of 2.5 mM Ca2+ to the medium. Mn2+ and Mg2+ were less effective in reversing the growth inhibition caused by Be2+ and the order of effectiveness was Ca2+ > Mn2+ > Mg2+. Fe3+ and Zn2+ were ineffective in reversing Be2+ toxicity.
Pyruvate, malate and succinate also reversed the growth inhibition caused by Be2+ inN. crassa. Pyruvate restored growth by a mechanism not involving control of Be2+ accumulation in the mould. The rate of utilisation of glucose by the mycelia grown in the presence of Be2+ was reduced, while that of pyruvate was not affected. The results indicate that the primary metabolic lesion in Be2+ toxicity inN. crassa is probably a block at some step(s) in the glycolytic sequence.
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References
Abelson, P. H. and Aldous, E. (1950)J. Bacteriol.,60, 401.
Adiga, P. R., Sivarama Sastry, K. and, Sarma, P. S. (1962)J. Gen. Microbiol.,29, 149.
Aldridge, W. N., Barnes, J. M. and Denz, F. A. (1949)Br. J. Pathol.,30, 375.
Aldridge, W. N. and Thomas, M. (1966)Biochem. J.,98, 100.
Cabrera, A. M. and West, T. S. (1963)Anal. Chem.,35, 311.
Castel, G. and Bertrand, D. (1954)Compt. Rend.,239, 1546.
Castel, G. (1956)Compt. Rend.,242, 2056.
Everest, D. A. (1964)The chemistry of beryllium (New York: Elsevier), p. 133.
Friedemann, T. E. and Haugen, G. E. (1943)J. Biol. Chem.,147, 115.
Hoagland, M. B. (1952)Arch. Biochem. Biophys.,35, 249.
Klemperrer, F. W. (1950)J. Biol. Chem.,187, 189.
Malmstrom, G. (1955)Arch. Biochem. Biophys.,58, 381.
Mukhina, S. T. (1967)Gig. Tr. Prof. Zabol.,11, 43.
Nelson, N. (1944)J. Biol. Chem.,153, 71.
Nirmala, J. and Sivarama Sastry, K. (1973)Biochem. J.,136, 749.
Sivarama Sastry, K., Raman, N., and Sarma, P. S. (1962a)Anal. Chem.,34, 1302.
Sivarama Sastry, K., Adiga, P. R., Venkatasubramanyam, V and Sarma, P. S. (1962),bBiochem. J.,85, 480.
Sivarama Sastry, K. (1963) inAdvances in biochemistry. Proc. of the first summer school in biochemistry, Srinagar (India) (Bangalore: Indian Institute of Science).
Steinberg, R. A. (1946)Am. J. Bot.,33, 210.
Thomas, M. and Aldridge,W. N. (1966)Biochem. J.,98, 94.
Witschi, H. P. (1970)Biochem. J.,120, 623.
Zalokar, M. (1954)Arch. Biochem. Biophys.,50, 71.
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Naidu, A., Nirmala, J. & Sastry, K.S. Beryllium toxicity inNeurospora crassa . J Biosci 1, 279–287 (1979). https://doi.org/10.1007/BF02716877
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DOI: https://doi.org/10.1007/BF02716877