Journal of Biosciences

, Volume 7, Issue 1, pp 39–47 | Cite as

Isolation, characterization and mapping of temperature-sensitive mutants of mycobacteriophage I3

  • Chanchal Sadhu
  • Mohan lal Gope
  • Krishna Sadhu
  • K. P. Gopinathan
Article

Abstract

Eighteen temperature-sensitive mutants of mycobacteriophage I3 have been isolated and partially characterized. All the mutants were defective in vegetative replication. Based on temperature shift experiments with the temperature sensitive mutants, the thermosensitive phase of the phage development period has been characterized for each mutant. The genes have been mapped by recombination analysis. The early, continuous and middle genes seem to cluster on the genetic map

Keywords

Mycobacteriophage I3 temperature sensitive mutants recombination analysis gene mapping 

Abbreviations used

ts

Temperature-sensitive

moi

multiplicities of infection

pfu

plaque forming unit

NTG

N-methyl-N′-nitro-N-nitrosoguanidine

KCN

potassium cyanide

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adams, M. H. (1959)Bacteriophages, (New York: Interscience).Google Scholar
  2. Brewer, G. J. (1978)Mol. Gen. Genet.,167, 65.PubMedGoogle Scholar
  3. Edgar, R. S. and Lielausis, I. (1964)Genetics,49, 649.PubMedGoogle Scholar
  4. Eisen, H. A., Fuerst, C. R., Siminovitch, L, Thomas, R., Lambert, L., Pereira Da Silva, L. and Jacob, F. (1966)Virology,30, 224.PubMedCrossRefGoogle Scholar
  5. Gopinathan, K. P. (1981)Curr. Sci.,50, 216.Google Scholar
  6. Gopinathan, K. P., Saroja, D., Gadagkar, R. and Ramakrishnan, T. (1978) inGenetics of Actinomycetales, (eds E. Freerkscen, I. Tarnok and J. H. Thumin) (New York: G. Fischer, Stuttgart), p. 237.Google Scholar
  7. Karnik, S. S. and Gopinathan, K. P. (1980)J. Virol.,33, 969.PubMedGoogle Scholar
  8. Levine, M. (1961)Virology,13, 493.PubMedCrossRefGoogle Scholar
  9. Lewin, B. (1977)Gene Expression. Plasmids and Phages (New York: John Wiley and Sons) Vol. 3, p. 565.Google Scholar
  10. Lindqvist, B. H. and Sinsheimer, R. L. (1967)J. Mol. Biol.,30, 69.PubMedCrossRefGoogle Scholar
  11. Mizuguchi, Y. and Sellers, M. I. (1970) inHost-Virus Relationship in Mycobacterium, Nocardia and Actinomycetes, (eds S. E. Juhasz and G. Plummer) (Springfield: Thomas, C. C.) p. 65.Google Scholar
  12. Nagaraja, V. (1981)Interaction between mycobacteriophage I3and its host Mycobacterium smegmatis, Ph.D. Thesis, Indian Institute of Science, Bangalore, India.Google Scholar
  13. Nagaraja, V. and Gopinathan, K. P. (1980)Arch. Microbiol.,124, 249.PubMedCrossRefGoogle Scholar
  14. Nagaraja, V. and Gopinathan, K. P. (1981a)FEBS Lett.,127, 57.PubMedCrossRefGoogle Scholar
  15. Nagaraja, V. and Gopinathan, K. P. (1981b)Mol. Biol. Rep.,8, 11.PubMedCrossRefGoogle Scholar
  16. Sadhu, C. (1984)Characterization of mycobacteriophage I3genome and its interaction with the host, Mycobacterium smegmatis, Ph.D. Thesis, Indian Institute of Science, Bangalore, India.Google Scholar
  17. Sunderraj, C. V. and Ramakrishnan, T. (1970)Nature (London),228, 280.Google Scholar

Copyright information

© Indian Academy of Sciences 1985

Authors and Affiliations

  • Chanchal Sadhu
    • 1
  • Mohan lal Gope
    • 1
  • Krishna Sadhu
    • 1
  • K. P. Gopinathan
    • 1
  1. 1.Microbiology and Cell Biology LaboratoryIndian Institute of ScienceBangaloreIndia

Personalised recommendations