Abstract
Isolated nuclei from differentiating cultures ofNicotiana sanderae showed increased levels of RNA polymerase activity as compared to the nuclei from callus cultures. The RNA synthetic activity was dependent on nucleotide triphosphates and Mg2+ and was destroyed by RNase. Maximum activity was obtained in the presence of 50 mM (NH4)2 SO4 and α-amanitin inhibited 40% and 55% of the activity in the nuclei from callus and differentiating tissue respectively. The nuclei from differentiating tissue elicited a 3-fold increase in RNA polymerase I and a 4-fold augmentation in RNA polymerase II activities.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- 2,4-D:
-
2,4-Dichlorophenoxy acetic acid
- NAA:
-
naphthalene acetic acid
- BAP:
-
benzyl aminopurine
- MS medium:
-
Murashige and Skoog’s medium
- MES:
-
2-(N-morpholinoethanesulphonic acid)
- GM:
-
grinding medium
- 5 mM MES-NaOH, pH 6.1, 4 mM magnesium acetate:
-
5 mM, 2 mercaptoethanol, 0.25 I sucrose, 4% gum arabic and 1% Triton X-100
References
Becker, W. M. (1979) inNucleic Acids in Plants, eds T. C. Hall and J. W. Davies (Florida: CRC press, Inc.) Vol. 1,p. 111.
Burkhanova, E. A., Fedina, A. B., Dmitrieva, G. B. and Kulaeva, G. N. (1980)Dokl. Akad. Nauk (USSR),252,488.
Chen, Y., Lin, C., Chang, H., Guilfoyle, T. J. and Key, J. L. (1975)Plant Physiol.,56,78.
Davies, E. and Larkins, B. A. (1973)Plant Physiol.,52,339.
Duda, C. T. (1976)Ann. Rev. Plant Physiol.,27, 119.
Fujimura, T., Komamine, A. and Matsumoto, H. (1980)Physiol. Plant.,49,255.
Giles, K. W. and Myers, A. (1965)Nature (London),206, 93.
Guilfoyle, T. J. and Hanson, J. B. (1973)Plant Physiol.,53,110.
Guilfoyle, T.J. and Key, J. L. (1977)Biochem. Biophys. Res. Commun.,74,308.
Guilfoyle, T. J., Olszeski, N. and Zurfluh, L. (1980) inGenome Organization and Expression in Plants, ed. C. J. Leaver (New York: Plenum Press) p. 93.
Hentschel, C. C. and Tata, J. R. (1977)Dev. Biol.,57,293.
Key, J. L. (1969)Ann. Rev. Plant Physiol.,20,449.
Mennes, A. M., Voogt, E. and Libbenga, K. R. (1977)Plant Sci. Lett.,8,171.
Murashige, T. and Skoog, F. (1962)Physiol. Plant,15,474.
Rizzo, P. J., Pedersen, K., and Cherry, J. H. (1977)Plant Sci. Lett.,8,205.
Sekiya, J. and Yamada, Y. (1978)Agric. Biol. Chem.,42,485.
Sengupta, C. and Raghaven, V. (1980)Exp. Bot.,31,347.
Widnell, C. C. and Tata, J. R. (1966)Biochem. Biophys. Acta,123,478.
Yu, F. L. (1975)Biochem. Biophys. Res. Commun.,64,1107.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Asha, P.K., Shaila, M.S., Vaidyanathan, C.S. et al. RNA polymerase activity in isolated nuclei ofNicotiana sanderae callus: Characteristics and modulation during differentiation. J Biosci 5, 347–353 (1983). https://doi.org/10.1007/BF02716701
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02716701