Abstract
Pulmonary macrophages of mice and hamsters but not rats have antigens on their surfaces which were absent from resident peritoneal macrophages, BCG stimulated pertioneal macrophages, and macrophages in other tissues. Antibodies to these antigens were found in the normal sera of rabbits from a variety of sources, and higher titers were induced by inoculation of rabbits with pulmonary macrophages. After absorption with peripheral blood cells and peritoneal macrophages, a highly specific anti-pulmonary macrophage serum was produced. Although usually absent in normal guinea pig sera, this antibody was induced in guinea pigs. To identify a specific antigen in mice, cell surface proteins of pulmonary and peritoneal macrophages were labelled with125I, disassociated by the detergent NP-40, and reacted with either specific guinea pig anti-mouse pulmonary macrophage serum or normal guinea pig serum. The precipitated surface proteins were identified by SDS-polyacrylamide gel electrophoresis. Specific pulmonary macrophage antigen had a molecular weight of 110,000. It was identified with specific antibody (but not with normal guinea pig serum) only on pulmonary macrophages. It was always absent from peritoneal macrophages. Proteins which precipitated in a non-specific fashion and were common to both pulmonary and peritoneal macrophages had molecular weights of 150,000–160,000, 70,000, and 32,000–40,0000. These proteins were detected with both the specific antiserum and normal guinea pig serum.
Similar content being viewed by others
References
Akagawa KS, Maruyama Y, Takano M, Kasai M, Tokunaga T (1981) A cell surface antigen expressed on mouse lung macrophages. Microbiol Immunol 25:1215–1220
Amos BD, Bashir H, Boyle W, MacQueen M, Tulikainen A (1969) A simple microcytotoxicity test. Transplantation 7:220–223
Balner H (1963) Identification of peritoneal macrophages in mouse radiation chimeras. Transplantation 1:217–223
Boltz-Nitulescu G, Foerster O (1979) Antigenic differences between alveolar and peritoneal macrophages of the rat. Lack of population specific determinants. Immunology 38:621–630
Boyse EA, Hubbard L, Stockert E, Lamm ME (1970) Improved complementation in the cytotoxic test. Transplantation 10:446–449
Brain JD, Frank NR (1968) Recovery of free cells from rat lungs by repeated washings. J Appl Physiol 25:63–69
Cleveland DW, Fischer SG, Kirschner MW, et al (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis of gel electrophoresis. J Biol Chem 253:1102–1106
Cohn ZA (1968) The structure and function of monocytes and macrophages. Adv Immunol 9:163–214
Cohn ZA, Weiner E (1963) The particulate hydrolases of macrophages. I. Comparative enzymology, isolation, and properties. J Exp Med 118:991–1008
Dauber JH, Daniele RP (1978) Chemotactic activity of guinea pig alveolar macrophages. Am Rev Respir Dis 117:673–684
Feldman JD, Tubergen DG, Pollack EM, Unanue ER (1972) Distribution of a macrophage-specific antigen. Cell Immunol 5:325–337
Ferrone S (1977) Rabbit complement in the lymphocytotoxicity test. Tissue Antigens 9:223–226
Ferrone S, Cooper NR, Pellegrino MA, Reisfeld RA (1973) Role of natural rabbit antibodies in the complement dependent cytotoxic reaction with human lymphoid cells. Fed Proc 32:1006 (abstr)
Ferrone S, Cooper NR, Pellegrino MA, Reisfeld RA (1974) The role of complement in the HL-A antibody mediated lysis of lymphocytes. Transplant Proc 6:13–19
Gallily R (1971) In vitro and in vivo studies of the properties and effects of antimacrophage sera (AS). Clin Exp Immunol 9:381–391
Godleski JJ, Brain JD (1972) The origin of alveolar macrophages in mouse radiation chimeras. J Exp Med 136:630–643
Goodman JW (1964) On the origin of peritoneal fluid cells. Blood 23:18–26
Gorenberg DJ, Daniele RP (1978) The alveolar macrophage: its capacity to act as an accessory cell in mitogen stimulated proliferation of guinea pig lymphocytes. Cell Immunol 36:115–127
Gorer PA, O’Gorman P (1956) The cytotoxic activity of isoantibodies in mice. Transplant Bull 3:142–143
Hopper KE, Wood PR, Nelson DS (1979) Macrophage heterogeneity. Vox Sang 36:257–274
Hubbard AL, Cohn ZA (1973) The enzymatic iodination of the red cell membrane. J Cell Biol 55:390–405
Hynes RO (1973) Alteration of cell surface proteins by viral transformation and by proteolysis. Proc Natl Acad Sci USA 70:3170–3174
Jett JH, Stevenson AP, Warner NL, Leary JF (1980) Quantitation of cell surface antigen density by flow cytometry. In: Laerum OD, Lindmo T, Thovud E (eds) Flow cytometry IV. Universitets Forlaget, Oslo, pp 215–221
Kaplan AM, Mohanakumar T (1977) Expression of a new cell surface antigen on activated murine macrophages. J Exp Med 146:1461–1466
Karnovsky ML, Simmons S, Glass EA, Shafer AW, D’Arcy Hart P (1970) Metabolism of macrophages. In: Van Furth R (ed) Mononuclear phagocytes. Blackwell, Oxford, pp 103–117
Laemmeli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Natúre 227:680–685
LeBlanc PA, Russell SW, Chang SMT (1982) Mouse mononuclear phagocyte antigenic heterogeneity detected by monoclonal antibodies. J Reticuloendothel Soc 32:219–231
Martinez RD, Montfort I (1973) A study of the specificity of alveolar macrophage antigen(s). Immunology 25:197–203
Montfort I, Perez-Tamayo R (1971) Two antigenically different types of macrophages. Proc Soc Exp Biol 138:204–207
Oren RA, Farnham AE, Saito K, Milofsky E, Karnovsky ML (1963) Metabolic patterns in three types of phagocytosing cells. J Cell Biol 17:487–501
Phillips DR, Morrison M (1971) Exposed protein on the intact human erythrocyte. Biochemistry 10:1766–1771
Rhodes J (1975) Macrophage heterogeneity in receptor activity: the activation of macrophage Fc recetor function in vivo and in vitro. J Immunol 114:976–981
Salzman GC, Hiebert RD, Crowell JM (1978) Data acquisition and display for a high-speed cell sorter. Comput Biomed Res 11:77–89
Schlesinger M (1965) Immune lysis of thymus and spleen cells of embryonic and neonatal mice. J Immunol 94:358–364
Schwartz BD, Nathanson SG (1971) Isolation of H-2 alicantigens solubilized by the detergent NP-40. J Immunol 107:1363–1367
Springer TA, Ho MK (1982) Macrophage differentiation antigens: markers of macrophage subpopulations and tissue localization. In: Mitchell MS, Oettgen HF (eds) Hybridomas in cancer: diagnosis and treatment. Raven Press, New York, pp 35–46
Stinnett JD, Kaplan AM, Morahan PS (1976) Identification of a macrophage-specific cell surface antigen. J Immunol 116:273–278
Unanue ER (1968) Properties and some uses of anti-macrophage antibodies. Nature 218:36–38
Van Furth R (1970) The origin and turnover of pro-monocytes, monocytes, and macrophages in normal mice. In: Van Furth R (ed) Mononuclear phagocytes. Davis, Philadelphia, pp 151–165
Wakefield JD, Batchelor JR (1966) The effect of natural antibody in guinea pig serum on mouse lymphoma cells in vitro and in vivo. Immunology 11:441–451
Weber I, Osborn M (1969) The reliability of molecular weight determination by dodecyl sulphate polyacylamide gel electrophoresis. J Biol Chem 244:4406–4412
Author information
Authors and Affiliations
Additional information
Supported in part by NIH grants HL 18339, HL 27244 and ES 0002
Rights and permissions
About this article
Cite this article
Godleski, J.J., Brain, J.D. Natural and induced antisera specific for pulmonary macrophages. Lung 162, 171–182 (1984). https://doi.org/10.1007/BF02715645
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02715645