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Biochemical and metabolic properties of bovine type II pneumocytes in primary culture

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Abstract

Bovine type II pneumocytes were isolated from lungs of adult cattle by enzymatic tissue dissociation and subsequent purification by density gradient centrifugation on a 1.040 g/ml Percoll gradient. By quantitative and qualitative high-performance liquid chromatographic (HPLC) analysis of freshly isolated, highly purified bovine type II pneumocytes, six phospholipid components (phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and sphingomyelin) were detected regularly and their composition was compared to that of whole lung homogenates. Total phospholipid content of 106 bovine type II cells was 34.7 µg (quantitative HPLC). Suspended and adherent bovine type II cells incorporated [14C]choline into their phospholipids. The rate of synthesis of suspended cells (289 pmol/h×106 cells) was constant for at least 4 h, while adherent type II cells incorporated [14C]choline with a constant rate of 160 pmol/h×106 cells for at least 20 h. These data further establish the use of cultured bovine type II pneumocytes as a high-purity model for the in vitro investigation of the surfactant system.

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Authors and Affiliations

  1. Institute of Pathology and Immunology Unit, School of Veterinary Medicine, Hannover, Federal Republic of Germany

    H. G. Augustin-Voss, H. A. Schoon, A. Gerull & S. Ueberschär

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  1. H. G. Augustin-Voss
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  2. H. A. Schoon
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  3. A. Gerull
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  4. S. Ueberschär
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Augustin-Voss, H.G., Schoon, H.A., Gerull, A. et al. Biochemical and metabolic properties of bovine type II pneumocytes in primary culture. Lung 167, 343–350 (1989). https://doi.org/10.1007/BF02714962

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  • DOI: https://doi.org/10.1007/BF02714962

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