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Cigarette smoke degrades hyaluronic acid

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Abstract

Cigarette smoke has been implicated in the pathogenesis of a variety of diseases that affect connective tissues. The effect of the gas phase of cigarette smoke on hyaluronic acid was investigated in vitro. The smoke markedly reduced the chain length of purified hyaluronic acid as assessed by both viscometry and gel chromatography. The hyaluronate exposed to smoke, nevertheless, could aggregate proteoglycans, although the viscosity of the aggregates was lower than in those formed with the unexposed material. The degradation of the hyaluronate was a rapid phenomenon and was inhibited by dimethylsulfoxide, a known scavenger of hydroxyl radicals. We conclude that free radicals in the gas phase of cigarette smoke degrade hyaluronic acid in vitro.

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Authors and Affiliations

  1. Departments of Musculoskeletal Research, Cleveland Clinic Foundation Research Institute, Cleveland, Ohio

    Cahir A. McDevitt

  2. Biostatistics and Epidemiology, Cleveland Clinic Foundation Research Institute, Cleveland, Ohio

    Gerald J. Beck

  3. Department of Pathology, School of Medicine, State University of New York at Stony Brook, Stony Brook, New York, USA

    Cahir A. McDevitt, Michael J. Ciunga & Jeffrey O’Brien

Authors
  1. Cahir A. McDevitt
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  2. Gerald J. Beck
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  3. Michael J. Ciunga
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  4. Jeffrey O’Brien
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McDevitt, C.A., Beck, G.J., Ciunga, M.J. et al. Cigarette smoke degrades hyaluronic acid. Lung 167, 237–245 (1989). https://doi.org/10.1007/BF02714952

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