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Biochemical definition of human tracheobronchial mucus

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Abstract

Tracheobronchial mucus is a heterogeneous milieu produced by several cell types. It has viscoelastic properties related to the organization of macromolecular components such as glycoproteins, proteins, lipids and nucleic acids. Proteins comprise components antigenically related to plasma proteins and secretory proteins such as kallicrein, lysozyme, amylase, bronchotransferrin, immunoglobulins A and proline-rich polypeptides. Tracheobronchial mucus also contains protease inhibitors and, in pathological conditions, important amounts of protease. Mucins, which are glycoproteins with a very rich carbohydrate content, represent another complex and major group of tracheobronchial molecules.

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References

  1. Afzelius, B.A.: A human syndrome caused by immotile cilia. Science193, 317–319 (1976)

    Article  PubMed  CAS  Google Scholar 

  2. Bailleul, V., Richet, C., Hayem, A., Degand, P.: Propriétés rhéologiques des sécrétions bronchiques: mise en évidence et rôle des polypeptides riches en proline (PRP). Clin. Chim. Acta74, 115–123 (1977)

    Article  PubMed  CAS  Google Scholar 

  3. Baker, A.P., Munro, J.R.: Multiglycosyltransferase system of canine respiratory tissue. J. Biol. Chem.246, 4358–4362 (1971)

    PubMed  CAS  Google Scholar 

  4. Baker, A.P., Sawyer, J.L., Munro, J.R., Weiner, G.P., Hillegass, L.M.: Glycosyltransferases of canine respiratory tissue. J. Biol. Chem.247, 5173–5179 (1972)

    PubMed  CAS  Google Scholar 

  5. Baker, A.P., Griggs, L.J., Munro, J.R., Finkelstein, J.A.: Blood group A active glycoproteins of respiratory mucus and their synthesis by an N-acetylgalactosaminyltransferase. J. Biol. Chem.248, 880–883 (1973)

    PubMed  CAS  Google Scholar 

  6. Baker, A.P., Chakrin, L.W., Wardell, J.R.: Chronic cholinergic stimulation of canine respiratory tissue. Its effects on the activities of glycosyltransferases and release of macromolecules. Am. Rev. Resp. Dis.111, 423–431 (1975)

    PubMed  CAS  Google Scholar 

  7. Baker, A.P., Sawyer, J.L.: Glycosyltransferases in human respiratory tissue. Biochem. Med.14, 42–50 (1975)

    Article  PubMed  CAS  Google Scholar 

  8. Baker, A.P., Hillegass, L.M., Holden, D.A., Smith, W.J.: Effect of kallidin, substance P and other basic polypeptides on the production of respiratory macromolecules. Am. Rev. Resp. Dis.115, 811–817 (1977)

    PubMed  CAS  Google Scholar 

  9. Baskerville, A.: The development and persistence of bronchial-gland hypertrophy and goblet-cell hyperplasia in the pig after injection of isoprenaline. J. Pathol.119, 35–47 (1976)

    Article  PubMed  CAS  Google Scholar 

  10. Bhattacharyya, S.N., Sahu, S., Lynn, W.S.: Structural studies on a glycoprotein isolated from alveoli of patients with alveolar proteinosis. Biochim. Biophys. Acta427, 91–106 (1976)

    PubMed  CAS  Google Scholar 

  11. Bhattacharyya, S.N., Lynn, W.S.: Structural studies on the oligosaccharides of a glycoprotein isolated from alveoli of patients with alveolar proteinosis. J. Biol. Chem.252, 1172–1180 (1977)

    PubMed  CAS  Google Scholar 

  12. Biserte, G., Havez, R., Cuvelier, R.: Les glycoprotéides des sécrétions bronchiques. Exp. Ann. Biochim. Med.24, 85–120 (1963)

    CAS  Google Scholar 

  13. Boat, T.F., Matthews, L.W.: Chemical composition of human tracheo bronchial secretions. In: Sputum, pp. 243–274 (ed. M.J. Dulfano). Springfield (Ill.): C.C. Thomas 1973

    Google Scholar 

  14. Boat, T.F., Kleinerman, J.L.: Human respiratory tract secretions. Effect of cholinergic and adrenergic agents on in vitro release of protein and mucous glycoprotein. Chest67 (Suppl.), 325–345 (1975)

    Article  Google Scholar 

  15. Boat, T.F., Cheng, P.W., Iyer, R.N., Carlson, D.M., Polony, I.: Human respiratory tract secretions. Arch. Biochem. Biophys.177, 95–104 (1976)

    Article  PubMed  CAS  Google Scholar 

  16. Bowes, D., Corrin, B.: Ultrastructural immunocytochemical localisation of lysozyme in human bronchial glands. Thorax32, 163–170 (1977)

    Article  PubMed  CAS  Google Scholar 

  17. Brandtzaeg, P.: Immunohistochemical studies on various aspects of glandular immunoglobulin transport in man. Histochem. J.9, 553–572 (1977)

    Article  PubMed  CAS  Google Scholar 

  18. Brogan, T.D.: The high molecular weight components of sputum. Brit. J. Exp. Pathol.41, 288–297 (1960)

    CAS  Google Scholar 

  19. Brogan, T.D., Ryley, H.C., Neale, L., Yassa, J.: Soluble proteins of bronchopulmonary secretions from patients with cystic fibrosis, asthma and bronchitis. Thorax30, 72–79 (1975)

    Article  PubMed  CAS  Google Scholar 

  20. Bukantz, S.C., Berns, A.W.: Studies with sputum. I - Initial observation on the chemical nature and blood group substance content of asthmatic sputum. J. Allergy29, 29–43 (1958)

    Article  CAS  Google Scholar 

  21. Bürgi, H., Wiesman, U., Richterich, R., Regli, J., Medici, T.: New objective criteria for inflammation in bronchial secretions. Brit. Med. J. 1968/II, 654-656

  22. Christensen, T.G., Korthy, A.L., Snider, G.L., Hayes, J.A.: Irreversible bronchial goblet-cell metaplasia in hamsters with elastase induced panacinar emphysema. J. Clin. Invest.59, 397–404 (1977)

    Article  PubMed  CAS  Google Scholar 

  23. Degand, P.: Examen biochimique des sécrétions bronchiques. Intérêt dans le traitement de l’hypersécrétion bronchique. In: Sécrétions et excrétions bronchiques. Monographies du Collège de Médecine des Hôpitaux de Paris (Expansion Scientifique), p. 32–36. Paris, 1976

  24. Degand, P., Roussel, P., Lamblin, G., Havez, R.: Purification et étude des mucines de kystes bronchogéniques. Biochim. Biophys. Acta320, 318–330 (1973)

    PubMed  CAS  Google Scholar 

  25. Degand, P., Ruffin, P., Lamblin, G., Havez, R.: Etude en ultracentrifugation de deux mucines bronchiques humaines. C. R. Acad. Sci. (Paris)276, 113–116 (1973)

    CAS  Google Scholar 

  26. Degand, P., Roussel, P., Lamblin, G.: Définition des mucines synthétisées au niveau de kystes bronchogéniques. Clin. Chim. Acta50, 223–236 (1974)

    Article  CAS  Google Scholar 

  27. Elliasson, R., Mossberg, B., Camner, P., Afzelius, B.A.: Immotile cilia, chronic airway infections and male sterility. N. Engl. J. Med.297, 1–6 (1977)

    Article  Google Scholar 

  28. Havez, R., Degand, P., Roussel, P., Biserte, G.: Isolement et caractérisation immunologique de la kallicréine bronchique humaine. C. R. Acad. Sci. (Paris)262, 309–311 (1966)

    CAS  Google Scholar 

  29. Havez, R., Roussel, P., Degand, P., Biserte, G.: Etude des structures fibrillaires de la sécrétion bronchique humaine. Clin. Chim. Acta17, 281–295 (1967)

    Article  PubMed  CAS  Google Scholar 

  30. Havez, R., Roussel, P., Degand, P., Delmas-Marsalet, Y., Biserte, G.: Etude des substances de groupe sanguin A isolées de mucus bronchique. Bull. Soc. Chim. Biol.51, 245–259 (1969)

    PubMed  CAS  Google Scholar 

  31. Havez, R., Roussel, P.: Bronchial mucus: physical and biochemical features. In: Bronchial Asthma, pp. 409–422 (eds. E.B. Weiss and M.S. Segal). Boston: Little, Brown and Co. 1976

    Google Scholar 

  32. Hochstrasser, K., Reichert, R., Schwarz, S., Werle, E.: Isolierung und Charakterisierung eines Protease Inhibitors aus menschlichem Bronchialsekret. Hoppe-Seyler’s Z. Physiol. Chem.353, 221–226 (1972)

    PubMed  CAS  Google Scholar 

  33. Hochstrasser, K., Reichert, R., Heimburger, N.: Antigenic relationship between the human bronchial mucus inhibitor and plasma inter α-trypsin inhibitor. Hoppe-Seyler’s Z. Physiol. Chem.354, 587–588 (1973)

    PubMed  CAS  Google Scholar 

  34. Hochstrasser, K., Reichert, R., Schwarz, S., Werle, E.: Detection and isolation of a second acid-stable proteinase inhibitor from human bronchial mucus. Hoppe Seyler’s Z. Physiol. Chem.354, 923–926 (1973)

    PubMed  CAS  Google Scholar 

  35. Hochstrasser, K., Schorn, K., Rasche, B., Lemparth, K., Raffelt, C.: Characterization of masked specific proteinase inhibitor from bronchial secretions in purulent sputum as complex with leucocytic proteinase. Pneumonologie152, 15–24 (1975)

    Article  PubMed  CAS  Google Scholar 

  36. Ishizaka, K., Newcomb, R.W.: Presence of γE in nasal washings and sputum from asthmatic patients. J. Allerg.46, 197–204 (1970)

    Article  PubMed  CAS  Google Scholar 

  37. Janoff, A., Sloan, B., Weinbaum, G., Damiano, V., Sandhaus, R.A., Elias, J., Kimbel, P.: Experimental emphysema induced with purified human neutrophil elastase: tissue localization of the instilled protease. Am. Rev. Respir. Dis.115, 461–478 (1977)

    PubMed  CAS  Google Scholar 

  38. Kaplan, P.D., Kuhn, C., Pierce, J.A.: The induction of emphysema with elastase. I - The evolution of the lesion and the influence of serum. J. Lab. Clin. Med.82, 349–356 (1973)

    PubMed  CAS  Google Scholar 

  39. Klockars, M., Reitamo, S.: Tissue distribution of lysozyme in man. J. Histochem. Cytochem.23, 932–940 (1975)

    PubMed  CAS  Google Scholar 

  40. Kollerstrom, N., Lord, P.W., Whimster, W.F.: A difference in the composition of bronchial mucus. Thorax32, 155–159 (1977)

    Article  PubMed  CAS  Google Scholar 

  41. Lafitte, J.J., Lamblin, G., Lhermitte, M., Humbert, P., Degand, P., Roussel, P.: Etude des mucines humaines obtenues par lavage de bronches macroscopiquement saines. Carbohyd. Res.56, 383–389 (1977)

    CAS  Google Scholar 

  42. Laine, A., Hayem, A.: Identification et caractérisation des constituants protéiques de la sécrétion bronchique humaine. Clin. Chim. Acta67, 159–167 (1976)

    Article  PubMed  CAS  Google Scholar 

  43. Laine, A., Hayem, A.: Constituants protéiques de liquides de lavages bronchiques humains. Bull. Europ. Physiopath. Resp.13, 47–56 (1977)

    CAS  Google Scholar 

  44. Lamb, D.: Acidic glycoproteins of the mucous glands of the bronchus in cystic fibrosis (mucoviscidosis). In: Colloq. Int. Pathol. Thorac. Lille (Hypersécrétion bronchique), pp. 143–153. Clichy, France: Poinsot, 1968

    Google Scholar 

  45. Lamb, D., Reid, L.: Histochemistry, autoradiography, tissue culture and biochemical analysis in the study of bronchial epithelial glycoproteins. In: Colloq. Int. Pathol. Thorax. Lille (Hypersécrétion bronchique), pp. 11–22. Clichy, France: Poinsot, 1968

    Google Scholar 

  46. Lamblin, G., Roussel, P., Mazzuca, M., Degand, P.: Action de la bradykinine et de différentes molécules basiques sur les battements ciliaires de la trachée de lapin. C. R. Soc. Biol. (Paris)166, 618–621 (1972)

    CAS  Google Scholar 

  47. Lamblin, G., Lhermitte, M., Degand, P., Sergeant, Y.H., Roussel, P.: Isolation and immunological properties of neutral bronchial mucins from a patient with chronic bronchitis. Biochim. Biophys. Acta322, 372–382 (1973)

    PubMed  CAS  Google Scholar 

  48. Lamblin, G., Lhermitte, M., Lafitte, J.J., Filliat, M., Degand, P., Roussel, P.: Etude comparative des mucines bronchiques purifiées à partir de l’expectoration de sujets atteints de mucoviscidose ou d’autres affections bronchiques chroniques. Bull. Europ. Physiopath. Resp.13, 175–190 (1977)

    CAS  Google Scholar 

  49. Lamblin, G., Humbert, P., Degand, P., Roussel, P.: Hétérogénéité des chaînes glycanniques des mucines bronchiques isolées à partir de l’expectoration de deux sujets atteints de bronchite chronique. Clin. Chim. Acta79, 425–436 (1977)

    Article  PubMed  CAS  Google Scholar 

  50. Lev, R., Spicer, S.S.: A histochemical comparison of human epithelia mucins in normal and in hypersecretory states including pancreatic cystic fibrosis. Am. J. Pathol.46, 23–47 (1965)

    PubMed  CAS  Google Scholar 

  51. Lewis, R.W.: Lipid composition of human bronchial mucus. Lipids6 859–861 (1971)

    Article  PubMed  CAS  Google Scholar 

  52. Lhermitte, M., Lamblin, G., Humbert, P., Roussel, P., Biserte, G.: Rôle d’adjuvant immunitaire des mucines bronchiques humaines à l’égard de protéines du sérum humain. C. R. Acad. Sci. (Paris)282, 1057–1058 (1976)

    Google Scholar 

  53. Lhermitte, M., Lamblin, G., Lafitte, J.J., Rousseau, J., Degand, P., Roussel, P.: Properties of human neutral bronchial mucins after modification of the peptide or the carbohydrate moieties. Biochimie58, 367–372 (1976)

    Article  PubMed  CAS  Google Scholar 

  54. Lhermitte, M., Lamblin, G., Degand, P., Roussel, P.: Affinity of bronchial secretion glycoproteins and cells of human bronchial mucosa for Ricinus communis lectins. Biochimie59, 611–620 (1977)

    Article  PubMed  CAS  Google Scholar 

  55. Lhermitte, M., Roche, A.C., Roussel, P., Mazzuca, M.: Affinity for human bronchial mucosa and secretions. In: Proc. of 4th International Symposium of Glycoconjugates (in press)

  56. Lieberman, J., Trimmer, B.M., Kurnick, N.B.: Substrate specificity of protease activities in purulent sputum. Lab. Invest.14, 249–257 (1965)

    PubMed  CAS  Google Scholar 

  57. Lieberman, J., Gaward, M.A.: Inhibitors and activators of leukocytic proteases in purulent sputum. Digestion of human lung inhibition by α1-antitrypsin. J. Lab. Clin. Med.77, 713–727 (1971)

    PubMed  CAS  Google Scholar 

  58. Lieberman, J., Kaneshiro, W.: Inhibition of leukocytic elastase from purulent sputum by α1-antitrypsin. J. Lab. Clin. Med.80, 88–101 (1972)

    PubMed  CAS  Google Scholar 

  59. Lorenz, T.H., Korst, D., Simpson, J.F., Musser, M.J.: A quantitative method of bronchial lysozyme I. An investigation of bronchial lysozyme. J. Lab. Clin. Med.49, 145–150 (1957)

    PubMed  CAS  Google Scholar 

  60. Louisot, P., Levrat, C., Gilly, R.: A new pathogenic hypothesis for cystic fibrosis: Hyperactivity of glycosyl transferases at microsomic level. Clin. Chim. Acta48, 373–376 (1973)

    Article  PubMed  CAS  Google Scholar 

  61. Martinez-Tello, F.J., Braun, D.G., Blanc, W.A.: Immunoglobulin production in bronchial mucosa and bronchial lymph nodes, particularly in cystic fibrosis of the pancreas. J. Immunol.101, 989–1003 (1968)

    PubMed  CAS  Google Scholar 

  62. Masson, P.L., Heremans, J.F.: Molecular size of γA-immunoglobulin from bronchial secretions. Biochim. Biophys. Acta120, 172–173 (1966)

    Article  PubMed  CAS  Google Scholar 

  63. Masson, P.L., Heremans, J.F., Prignot, J.J., Wauters, G.: Immunohistochemical localisation and bacteriostatic properties of an ironbinding protein from bronchial mucus. Thorax21, 538–541 (1966)

    Article  PubMed  CAS  Google Scholar 

  64. Masson, P.L., Heremans, J.F.: Sputum proteins. In: Sputum, pp. 412–475 (ed. M.J. Dulfano). Springfield (Ill.): C.C. Thomas 1973

    Google Scholar 

  65. Mazzuca, M., Roche, A.C., Lhermitte, M., Roussel, P.: Limulus polyphemus lectin sites in human bronchial mucosa. J. Histochem. Cytochem.25, 470–473 (1977)

    PubMed  CAS  Google Scholar 

  66. Meyer, F.A.: Comparison of structural glycoproteins from mucus of different sources. Biochim. Biophys. Acta493, 272–282 (1977)

    PubMed  CAS  Google Scholar 

  67. Ohlsson, K., Olsson, I.: The neutral proteases of human granulocytes. Isolation and partial characterization of two granulocytes collagenases. Europ. J. Biochem.36, 473–481 (1975)

    Article  Google Scholar 

  68. Ohlsson, K., Tegner, H.: Granulocytes collagenase, elastase and plasma protease inhibitors in purulent sputum. Europ. J. Clin. Invest.5, 221–227 (1975)

    Article  PubMed  CAS  Google Scholar 

  69. Ohlsson, K., Tegner, H.: Inhibition of elastase from granulocytes by the low molecular weight bronchial protease inhibitor. Scand. J. Clin. Lab. Invest.36, 437–445 (1976)

    Article  PubMed  CAS  Google Scholar 

  70. Passero, M.A., Ross, W.T., Kilburn, K.H., Lynn, W.S.: Isolation and characterization of two glycoproteins from patients with alveolar proteinosis. Proc. Natl. Acad. Sci. USA70, 973–976 (1973)

    Article  PubMed  CAS  Google Scholar 

  71. Potter, J.L., Matthews, L.W., Lemm, J., Spector, S.: Human pulmonary secretions in health and disease. Ann. N.Y. Acad. Sci.106, 692–697 (1963)

    Article  PubMed  CAS  Google Scholar 

  72. Puchelle, E., Zahm, J.M., Havez, R.: Biochemical and rheological data in sputum. III. Relationship between the biochemical constituents and the rheological properties of sputum. Bull. Physiopath. Resp.9, 237–256 (1973)

    CAS  Google Scholar 

  73. Puchelle, E., Girard, F., Beck, G., Hayem, A., Bailleul, V., Laine, A.: Propriétés rhéologiques et biochimiques de l’expectoration. Path. Biol.24, 93–96 (1976)

    CAS  Google Scholar 

  74. Ramirez, R.J., Harlan, W.R.: Pulmonary alveolar proteinosis. Am. J. Med.45, 502–512 (1968)

    Article  PubMed  CAS  Google Scholar 

  75. Reid, L.: Measurement of the bronchial mucous gland layer: a diagnostic yardstick in chronic bronchitis. Thorax15, 132–141 (1960)

    Article  PubMed  CAS  Google Scholar 

  76. Reid, L.: Natural history of mucus in the bronchial tree. Arch. Environ. Health10, 265–273 (1965)

    PubMed  CAS  Google Scholar 

  77. Reid, L., De Haller, R.: The bronchial mucous glands. Their hypertrophy and change in intracellular mucus. Bibl. Paediatr.86, 195–199 (1967)

    PubMed  CAS  Google Scholar 

  78. Reid, L.: Disturbances in the pattern of secretion of bronchial mucous glands. In: Cystic Fibrosis, Ciba Foundation no. 32, pp. 45–67, (eds. Porter R., O’Connor, M.). Boston: Little Brown and Co. 1968

    Google Scholar 

  79. Reid, L.: Histopathological aspects of bronchial secretion. Scand. J. Resp. Dis. (Supplem. no 90), 9–15 (1974)

    Google Scholar 

  80. Reynolds, H.Y., Newball, H.H.: Analysis of proteins and respiratory cells obtained from human lungs by bronchial lavage. J. Lab. Clin. Med. 84, 559–573 (1974)

    PubMed  CAS  Google Scholar 

  81. Reynolds, H.Y., Fulmer, J.D., Kazmierowski, J.A., Roberts, W.C., Frank, M., Crystal, R.G.: Analysis of cellular and protein content of bronchoalveolar lavage fluid from patients with idiopathic pulmonary fibrosis and chronic hypersensivity pneumonitis. J. Clin. Invest.59, 165–175 (1977)

    Article  PubMed  CAS  Google Scholar 

  82. Roberts, G.P.: Isolation and characterization of glycoproteins from sputum. Europ. J. Biochem.50, 265–280 (1974)

    Article  PubMed  CAS  Google Scholar 

  83. Roberts, G.P.: The role of disulfide bonds in maintaining the gel structure of bronchial mucus. Arch. Biochem. Biophys.173, 528–537 (1976)

    Article  PubMed  CAS  Google Scholar 

  84. Roussel, P., Lamblin, G., Degand, P., Havez, R.: Isolement des mucines bronchiques sécrétées au cours de la mucoviscidose. Clin. Chim. Acta.36, 315–328 (1972)

    Article  PubMed  CAS  Google Scholar 

  85. Roussel, P., Lamblin, G., Degand, P., Walker-Nasir, E., Jeanloz, R.W.: Heterogeneity of the carbohydrate chains of sulfated bronchial glycoproteins isolated from a patient suffering from cystic fibrosis. J. Biol. Chem.250, 2114–2122 (1975)

    PubMed  CAS  Google Scholar 

  86. Ryley, H.C.: An immunoelectrophoretic study of the soluble secretory proteins of sputum. Biochim. Biophys. Acta271, 300–309 (1972)

    CAS  Google Scholar 

  87. Sahu, S., Di Augustine, R.P., Lynn, W.S.: Lipids found in pulmonary lavage of patients with alveolar proteinosis and in rabbit lung lamellar organelles. Am. Rev. Resp. Dis.114, 177–184 (1976)

    PubMed  CAS  Google Scholar 

  88. Sahu, S., Lynn, W.S.: Lipid composition of airway secretions from patients with asthma and patients with cystic fibrosis. Am. Rev. Resp. Dis.115, 233–239 (1977)

    PubMed  CAS  Google Scholar 

  89. Sheffner, A.L.: The reduction in vitro in viscosity of mucoprotein solution by a new mucolytic agent, N-acetylcysteine. Ann. N.Y. Acad. Sci.106, 298–310 (1963)

    Article  PubMed  CAS  Google Scholar 

  90. Smith, J.M.: Interference with tryptic digestion by sputum from asthmatic patients. Am. Rev. Resp. Dis.88, 858–860 (1963)

    PubMed  CAS  Google Scholar 

  91. Spicer, S.S., Chakrin, L.W., Wardell, J.: Respiratory mucous secretion. In: Sputum, pp. 22–68 (ed. M.J. Dulfano). Springfield (Ill.): C.C. Thomas 1973

    Google Scholar 

  92. Stahl, G.H., Ellis, D.B.: Biosynthesis of respiratory-tract mucins. A comparison of canine epithelial goblet-cell and submucosal-gland secretions. Biochem. J.136, 845–850 (1973)

    PubMed  CAS  Google Scholar 

  93. Sturgess, J., Reid, L.: Secretory activity of the human bronchial mucous glands in vitro. Exp. Mol. Path.16, 362–381 (1972)

    Article  CAS  Google Scholar 

  94. Sturgess, J., Reid, L.: An organ culture study of the effect of drugs on the secretory activity of the bronchial submucosal gland. Clin. Sci.43, 533–543 (1972)

    PubMed  CAS  Google Scholar 

  95. Sturgess, J., Reid, L.: The effect of isoprenaline and pilocarpine on bronchial mucus secreting tissue and pancreas, salivary glands, heart, thymus, liver and spleen. Brit. J. Exp. Path.54, 388–403 (1973)

    PubMed  CAS  Google Scholar 

  96. Tegner, H., Ohlsson, K.: Localization of a low molecular weight protease inhibitor to tracheal and maxillary sinus mucosa. Hoppe-Seyler’s Z. Physiol. Chem.358, 425–429 (1977)

    PubMed  CAS  Google Scholar 

  97. Tegner, H., Ohlsson, K., Olsson, I.: The interactions between a low molecular weight protease inhibitor of bronchial mucus and chymotrypsin-like cationic proteins of granulocytes. Hoppe-Seyler’s Z. Physiol. Chem.358, 431–433 (1977)

    PubMed  CAS  Google Scholar 

  98. Toremtram, N.C.: The daily amount of tracheo-bronchial secretions in man. Acta Otolaryngol. (Stockh.)158 (Suppl.), 43–53 (1960)

    Google Scholar 

  99. Tourville, D.R., Adler, R.H., Bienenstock, J., Tomasi, T.B.: The human secretory immunoglobulin system. Immunohistological localization of γA "secretory piece" and lactoferrin in normal tissues. J. Exp. Med.129, 411–423 (1969)

    Article  PubMed  CAS  Google Scholar 

  100. Twumasi, D.Y., Liener, I.E.: Protease from purulent sputum purification and properties of the elastase and chymotrypsin-like enzymes. J. Biol. Chem.252, 1917–1926 (1977)

    PubMed  CAS  Google Scholar 

  101. Warembourg, H., Havez, R., Sezille, G., Sherpereel, P., Roussel, P. Degand, P.: Les lipides de l’expectoration. Isolement et caractérisation du surfactant pulmonaire dans l’expectoration. In: Hypersecrétion Bronchique, Colloq. Int. Pathol. Thorac., Lille, pp. 181–192. Clichy, France: Poinsot, 1968

    Google Scholar 

  102. Warr, G.A., Martin, R.R., Sharp, P.M., Rossen, R.D.: Normal human immunoglobulins and proteins. Am. Rev. Resp. Dis.116, 25–30 (1977)

    PubMed  CAS  Google Scholar 

  103. Whimster, W.F., Reid, L.: The influence of dibutyryl cyclic adenosine monophosphate and other substances on human bronchial mucous gland discharge. Exp. Mol. Pathol.18, 234–240 (1973)

    Article  PubMed  CAS  Google Scholar 

  104. Yeager, H.: Tracheobronchial secretions. Am. J. Med.50, 493–509 (1971)

    Article  PubMed  CAS  Google Scholar 

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  1. J. J. Lafitte (Boursier de l’Association Française de Lutte contre la Mucoviscidose)

    Present address: Clinique de Pneumologie, UER 2 de Médecine de Lille, France

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  1. Unité de Recherches No. 16 I.N.S.E.R.M. Place de Verdun, F-59045, Lille Cédex, France

    P. Roussel, P. Degand, G. Lamblin, A. Laine & J. J. Lafitte (Boursier de l’Association Française de Lutte contre la Mucoviscidose)

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  1. P. Roussel
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The research of the authors was supported by Contrat de Recherches UER 3 de Lille, Contrat INSERM no 77-5-207-3, and Contrat CECA no 7246-22-3-00-3

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Roussel, P., Degand, P., Lamblin, G. et al. Biochemical definition of human tracheobronchial mucus. Lung 154, 241–260 (1976). https://doi.org/10.1007/BF02713541

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