Abstract
Nick translation of intact rat heart nuclei has shown that the incorporation of [3H]-dATP is greater in hypertrophic heart nuclei than in normal heart nuclei suggesting that hypertrophic heart nuclei have more DNase I sensitive regions than normal heart nuclei. DNase I sensitivity analysis has shown that the rate and extent of digestion of myosin heavy chain genes are greater in hypertrophic than in normal heart nuclei. Dot blot hybridization analysis of myosin heavy chain transcripts from hypertrophic heart nuclei using myosin heavy chain cDNA as probe has shown that the sensitivity of myosin heavy chain genes to DNase I in hypertrophic heart nuclei correlates with myosin heavy chain gene activation and increased number of transcripts.
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References
Burton, K. (1956)Biochem. J.,62, 315.
Chaturvedi, M. M. and Kanungo, M. S. (1985)Mol. Biol. Rep.,10, 215.
Dimitriadis, G. J. and Tale, J. R. (1980)Biochem. J.,187, 467.
Folger, K., Anderson, J. N., Hayward, M. A. and Shapiro, D. J. (1983)J. Biol. Chem.,258, 1973.
Hutchison, N. and Weintraub, H. (1985)Cell. 43, 471.
Jackowski, G. and Liew, C. C. (1980)Biochem. J.,188, 363.
Kamala, J., Mariappan, M. and Rajamanickam, C. (1986)Biochem. Int.,13, 271.
Koropatnick, J. and Duerksen, J. D. (1987)Dev. Biol.,122, 1.
Levitt, A., Axel, R. and Cedar, H. (1979)Dev. Biol.,69, 496.
Limas, C. J. (1982)Biochem. Biophys. Acta,696, 37.
Lompre, A. M., Nadal-Ginard, B. and Mahdavi, V. (1984).J. Biol Chem.,259, 6437.
Lutter, L. C. (1978)J. Mol. Biol.,124, 391.
Mahdavi, V., Periasamy, M. and Nadal-Ginard, B. (1982)Nature (London),297, 659.
Meenakshi, S., Thirunavukkarasu, C. and Rajamanickam, C. (1983)Biochem. J.,209, 285.
Nicolas, G. H., Wright, C. A., Cockerill, P. N. and Wyke, J. A. (1983)Nucleic Acids Res.,11, 753.
Noll, M. (1974)Nature (London),251, 249.
Penmann, S. (1966)J. Mol. Biol,17, 117.
Rakusan, K. and Poupa, D. (1966)Cardiologia,69, 263.
Ravisankar, H. and Padmanaban, G. (1985)J. Biol. Chem.,260, 1588.
Rigby, P. W. J., Dieckmann, M., Rhodes, C. and Berg, P. (1977)Cell,10, 537.
Sollner-Webb, B. and Felsenfeld, G. (1977)Cell,10, 537.
Stalder, J., Larsen, A., Engle, J. D., Dolan, M., Groudine, M. and Weintraub, A. (1980)Cell,20, 451.
Strobe, U., Wilson, R., Seling, E. and Walfield, A. (1981)Biochemistry,20, 990.
Weintraub, H. and Groudine, M. (1976)Science,193, 848.
Zak, R. and Rabinowitz, M. (1979)Annu. Rev. Physiol.,41, 539.
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Selvamurugan, N., Kamala, J. & Rajamanickam, C. Activation of myosin heavy chain genes during cardiac hypertrophy. J Biosci 13, 249–256 (1988). https://doi.org/10.1007/BF02712148
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DOI: https://doi.org/10.1007/BF02712148