Abstract
The concentration- and time-dependence of interfacial pressure of nisin and β-casein at ann-hexadecane-water interface were evaluated by using DuNoüy tensiometry. The two emulsifiers attained interfacial saturation at a bulk concentration of about 0.1 mg/ml, the reduction of the interfacial tension by nisin at that concenration being about equivalent to that of β -casein. The time dependence of interfacial tension recorded for each protein was described by using two kinetic models. In the first, the reduction of interfacial tension with time was considered to be a result of molecular penetration into the interface followed by rearrangement. Nisin exhibited more rapid penetration and rearrangement at the interface than did β -casein. In the second, the model allowed for the parallel, irreversible adsorption of protein into each of two states from solution, where state 2 molecules occupy greater interfacial area and are more tightly bound than state 1 molecules. The extent of adsorption in state 1 and state 2 was determined to be highly concentration dependent for each protein; adsorption occurs mostly in state 1 at high concentration and mostly in state 2 at low concentrations.
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Andarde, J. D., Hlady, V. L. and Van Wagenen, R. A., “Effects of Plasma Protein Adsorption on Protein Conformation and Activity,”Pure&Appl. Chem.,56, 1345 (1984).
Bani-Jaber, A., McGuire, J., Ayres, J. and Daeschel, M., Submitted for Publication. Bower, C. K., Ph. D. Dissertation, Oregon State University, Corvallis (1994).
Cho, D., Narsimhan, G. and Franses, E. I., “Adsorption Dynamics of Native and Alkylated Derivatives of Bovine Serum Albumin at Air-water Interfaces,”J. Colloid Interface Sci.,178, 348 (1996).
CSC Scientific Co., Inc., Operating Instructions: CSC-DuNouy Tensiometer CSC Nos 70535 and 70545. CSC Scientific, Co., Inc., Fairfax, VA (1988).
Dickinson, E. and Matsumura, Y., “Proteins at Liquid Interfaces: Role of the Molten Globule State,”Colloids and Surfaces B: Biointerfaces,3, 1 (1994).
Farooq, U. and Narsimhan, G., “A Surface Equation of State for Globular Proteins at the Air-water Interface,”J. Colloid Interface Sci.,146, 169 (1991).
Graham, D. E. and Philips, M. J., “Proteins at Liquid Interfaces. I: Kinetics of Adsorption and Surface Denaturation,”J. Colloid Interface Sci.,70, 403 (1979).
Guzman, R. Z., Carboonell, R. G. and Kilpatrick, P. K., “The Adsorption of Proteins to Gas-liquid Interfaces,”J. Colloid Interface Sci.,114, 536 (1986).
Hunter, J. R., Kilpatrick, P. K. and Carbonell, R. G., “β -Casein Adsorption at the Air/Water Interface,”J. Colloid Interface Sci.,142, 429 (1991).
Hurst, A., “Nisin,”Adv. Appl. Microbiol,27, 85 (1981).
Krisdhasima, V., Vinaraphong, P. and McGuire, J., “Adsorption Kinetics and Elutability of α-Lactalbumin, β -Casein, β -Lactoglobulin, and Bovine Serum Albumin, at Hydrophobie and Hydrophilic Interfaces,”J. Colloid Interface Sci.,161, 325 (1993).
Lakamraju, M., McGuire, J. and Daeschel, M., “Nisin Adsorption and Exchange with Selected Milk Proteins at Silanized Silica Surface,”J. Colloid Interface Sci.,178, 495 (1996).
Lee, W. K., McGuire, J. and Bothwell, M. K., “A Mechanistic Approach to Modeling Single Protein Adsorption at Solid-Water Interfaces,”J. Colloid Interface Sci.,213, 265 (1999).
Ross, S. and Morrison, I., “Colloid Systems and Interfaces” John Wiley and Sons, N.Y., 69 (1988).
Song, K. B. and Damodaran, S., “Influence of Electrostatic Forces on the Adsorption of Succinylated β-Lactoglobulin at the Airwater Interface”Langmuir,7, 2737 (1991).
Van De Ven, F J. M., van den Hooven, R. N. H., Konings and Hilbers, C. W., G. Jung and H. G. Sahl (Eds), “Nisin and Novel Lantibiotics,” ESCOM Science Publishers B.V, The Netherlands, 35 (1991).
Wang, J. and McGuire, J., “Surface Tension Kinetics of the Wild Type and Four Synthetic Stability Mutants of T4 Phage Lysozyme at the Air-water Interface,”J. Colloid Interface Sci.,185, 317 (1997).
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Lee, WK., Bani-Jaber, A., McGuire, J. et al. Interfacial tension kinetics of nisin and β-Casein at an oil-water interface. Korean J. Chem. Eng. 17, 179–183 (2000). https://doi.org/10.1007/BF02707140
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DOI: https://doi.org/10.1007/BF02707140