Abstract
Taxol, a powerful anticancer agent, was extracted from yew extract and separated by NP-HPLC (NormalPhase High Performance Liquid Chromatography). At the mobile phase composition of hexane/IPA/MeOH, 90/4/6 vol%, taxol was preparatively separated in a chromatographic column with large packings (15 μm). The purified taxol was used to determine the adsorption isotherm of taxol. From the experimental results, the adsorption isotherm was obtained as the following Langmuir type,
To obtain the adsorption isotherm, only two injections of a small and large amount of taxol were required, and the Langmuir adsorption parameters were calculated from the assumption of monolayer coverage on the solid surface.
References
Cardellina II, J. H., “HPLC Separation of Taxol and Cephalomannine,”J. of Liquid Chromatogr.,14, 659 (1991).
Chang, K. K., Park, Y. K., Row, K. H. and Chung, S. T., “Pretreatment Step in Separation Process for Taxol,”Chemical Industry and Technology,16, 1 (1998).
Chang, K. K., Row, K. H. and Chung, S. T., “Separation of Taxol from Taxanes by NP-HPLC,”J. of The Korean Ind. & Eng. Chemistry,8, 286 (1997).
Hadden, N., Baumann, F., MacDonald, F., Munk, M., Stevenson, R., Gere, D., Zamaroni, F. and Majors, R., “Basic Liquid Chromatography,” Varian Aerograph (1971).
Jandera, P., Posvec, Z. and Vraspir, P., “Mobile Phase Effects on Single-Component and Competitive Adsorption Isotherms in Reversed-Phase Systems,”J. of Chromatogr. A,734, 125 (1996).
Jung, Y. A., Chung, S. T. and Row, K. H., “Resolution of Taxol in Terms of Mobile Phase Compositions in RP-HPLC,”HWAHAK KONGHAK,35, 712 (1997).
Rao, K. V., Reddy, G. C. and Juchum, J., “Taxanes of the Needles ofTaxus X Media,”Physicochemistry,43, 439 (1996).
Snyder, L. R., Cox, G. B. and Antle, P. E., “A Simplified Description of HPLC Separation under Overload Conditions. A Synthesis and Extension of Two Recent Approaches,”Chromatographia,24, 82 (1987).
Sofer, G. and Hagel, L., “Handbook of Process Chromatography: A Guide to Optimization, Scale up, and Validation,” Academic Press, San Diego (1997).
Wu, D. R., Lohse, K. and Greenblatt, H. C., “Preparative Separation of Taxol in Normal and Reversed-Phase Operations,”J. of Chromatogr. A,702, 233 (1995).
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Park, Y.K., Chung, S.T. & Row, K.H. A simple method to determine the adsorption isotherm of taxol from a yew tree. Korean J. Chem. Eng. 16, 388–391 (1999). https://doi.org/10.1007/BF02707130
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DOI: https://doi.org/10.1007/BF02707130