Skip to main content
SpringerLink
Log in

Phosphate deficiency-dependent upregulation of UDP-glucose pyrophosphorylase genes is insensitive to ABA and ethylene status in Arabidopsis leaves

  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

The effects of inorganic phosphate (Pi) deficiency and ABA/ethylene status on expression of UDP-glucose pyrophosphorylase (UGPase) genes (Ugp), involved in sucrose/polysaccharide metabolism, were investigated. Both wild-type (wt), aba and abi mutants (ABA-deficient and -in-sensitive), etr, ein and eto (ethylene resistant and overproducing) grown on Pi-deficient and complete nutrient solution, as well as phol (Pi-deficient) mutants of Arabidopsis thaliana were used for experiments. Generally, Pi-deficiency conditions (including mannose feeding to decrease cytosolic Pi pool) resulted in an increase of Ugp expression in the leaves, under all experimental conditions. Mutant backgrounds reflecting differences in ABA or ethylene status/ sensitivity had no effect on the level of Ugp up-regulation by Pi-stress. Furthermore, feeding ABA to the leaves of wt and pho1 plants had no effect on Ugp expression, regardless of the sucrose status in the leaves. The data suggest that Pi deficiency leading to up-regulation of Ugp acts independently of ABA and ethylene status.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

aba :

abscisic acid-deficient mutants

abi :

ABA-insensitive mutants

Pi:

inorganic phosphate

-P:

phosphorus-deficient plants

+P:

control plants

pho1 :

phosphate-deficient mutant

UGPase:

UDP-glucose pyrophosphorylase

References

  • Abel S., Ticconi C.A., Delatorre C.A. 2002. Phosphate sensing in higher plants. Physiol. Plant. 115: 1–8.

    Article  PubMed  CAS  Google Scholar 

  • Arenas-Huertero F., Arroyo A., Zhou L., Sheen J., León P. 2000. Analysis of Arabidopsis glucose sensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar. Genes Dev. 14: 2085–2096.

    PubMed  CAS  Google Scholar 

  • Borch K., Bouma T.J., Lynch J.P., Brown K.M. 1999. Ethylene: a regulator of root architectural responses to soil phosphorus availability. Plant Cell Environ. 22: 425–431.

    Article  CAS  Google Scholar 

  • Ciereszko I., Barbachowska A. 2000. Sucrose metabolism in leaves and roots of bean (Phaseolus vulgaris L.) during phosphate deficiency. J. Plant Physiol. 156: 640–644.

    CAS  Google Scholar 

  • Ciereszko I., Janonis A., Kociakowska M. 2002. Growth and metabolism of cucumber (Cucumis sativus L.) in phosphate-deficient conditions. J. Plant Nutr. 25: 1115–1127.

    Article  CAS  Google Scholar 

  • Ciereszko I., Johansson H., Hurry V., Kleczkowski L.A. 2001a. Phosphate status affects the gene expression, protein content and enzymatic activity of UDP-glucose pyrophosphorylase in wild-type and pho mutants of Arabidopsis. Planta 212: 598–605.

    Article  PubMed  CAS  Google Scholar 

  • Ciereszko I., Johansson H., Kleczkowski L.A. 2001b. Sucrose and light regulation of a cold-inducible UDP-glucose pyrophosphorylase gene via a hexokinase-independent and abscisic acid-insensitive pathway in Arabidopsis. Biochem J. 354: 67–72.

    Article  PubMed  CAS  Google Scholar 

  • Ciereszko I., Johansson H., Kleczkowski L.A. 2005. Interactive effects of phosphate deficiency, sugar and light/dark conditions on gene expression of UDP-glucose pyrophosphorylase in Arabidopsis. J. Plant Physiol. 162: 343–353.

    Article  PubMed  CAS  Google Scholar 

  • Ciereszko I., Kleczkowski L.A. 2002. Effects of phosphate deficiency and sugars on expression of rab18 in Arabidopsis: hexokinase-dependent and okadaic acid-sensitive transduction of the sugar signal. Biochim. Biophys. Acta 1579: 43–49.

    PubMed  CAS  Google Scholar 

  • Ciereszko I., Kleczkowski L.A. 2005. Expression of several genes involved in sucrose/starch metabolism as affected by different strategies to induce phosphate deficiency in Arabidopsis. Acta Physiol. Plant. 27: 147–155.

    Article  CAS  Google Scholar 

  • Ciereszko I., Miłosek I., Rychter A.M. 1999. Assimilate distribution in bean plants (Phaseolus vulgaris L.) during phosphate deficiency. Acta Soc. Bot. Pol. 68: 269–273.

    Google Scholar 

  • Franco-Zorilla J.M., González E., Bustos R., Linhares F., Leyva A., Paz-Ares J. 2004. The transcriptional control of plant responses to phosphate limitation. J. Exp. Bot. 55: 285–293.

    Article  Google Scholar 

  • Gazzarrini S., McCourt P. 2001. Genetic interaction between ABA, ethylene and sugar signaling pathways. Curr. Opin. Plant Biol. 4: 387–391.

    Article  PubMed  CAS  Google Scholar 

  • Gibson S.I. 2004. Sugar and phytohormone response pathways: navigating a signalling network. J. Exp. Bot. 55: 253–264.

    Article  PubMed  CAS  Google Scholar 

  • Huijser C., Kortstee A., Pego J., Weisbeek P., Wisman E., Smeekens S. 2000. The Arabidopsis SUCROSE COUPLED-6 gene is identical to ABSCISIC ACID INSENSITIVE-4: involvement of abscisic acid in sugar responses. Plant J. 23: 577–585.

    Article  PubMed  CAS  Google Scholar 

  • Jeschke W.D., Peuke A.D., Pate J.S., Hartung W. 1997. Transport, synthesis and catabolism of abscisic acid (ABA) in intact plants of castor bean (Ricinus communis L.) under phosphate deficiency and moderate salinity. J. Exp. Bot. 48: 1737–1747.

    Article  CAS  Google Scholar 

  • Johansson H. 2003. Gene regulation of UDP-glucose synthesis and metabolism in plants. Doctoral dissertation, UPSC, Department of Plant Physiology, Ume University, Sweden, ISBN 91-7305-394-5.

    Google Scholar 

  • Kleczkowski L.A., Geisler M., Ciereszko I., Johansson H. 2004. UDP-glucose pyrophosphorylase — an old protein with new tricks. Plant Physiol. 134: 912–918.

    Article  PubMed  CAS  Google Scholar 

  • Koornneef M., Jorna M.L., Brinkhorst-van der Swan D.C.L., Karssen C.M. 1982. The isolation of abscisic acid (ABA) deficient mutants by selection of induced revertants in nongerminating gibberellin sensitive lines of Arabidopsis thaliana (L) Heynh. Theor. Appl. Genet. 61: 382–396.

    Google Scholar 

  • Kuiper D., Staal M. 1987. The effect of exogenously applied plant growth substances on the physiological plasticity in Plantago major pleiosperma. Responses of growth, shoot to shoot ratio and respiration. Physiol. Plant. 69: 651–658.

    Article  CAS  Google Scholar 

  • López-Bucio J., Hernández-Abreu E., Sánchez-Calderón L., Nieto-Jacobo M.F., Simpson J., Herrera-Estrella L. 2002. Phosphate availability alters architecture and causes changes in hormone sensitivity in the Arabidopsis root system. Plant Physiol. 129: 244–256.

    Article  PubMed  CAS  Google Scholar 

  • Martin A.C., del Pozo J.C., Iglesias J., Rubio V., Solano R., de la Pea A., Leyva A., Paz-Ares J. 2000. Influence of cytokinins on the expression of phosphate starvation responsive genes in Arabidopsis. Plant J. 24: 559–567.

    Article  PubMed  CAS  Google Scholar 

  • Moore B., Zhou L., Rolland F., Hall Q., Cheng W.-H., Liu Y.-X., Hwang I., Jones T., Sheen J. 2003. Role of the Arabidopsis glucose sensor HXK1 in nutrient, light, and hormonal signaling. Science 300: 332–336.

    Article  PubMed  CAS  Google Scholar 

  • Poirier Y., Thoma S., Somerville C., Schiefelbein J. 1991. A mutant of Arabidopsis deficient in xylem loading of phosphate. Plant Physiol. 97: 1087–1093.

    Article  PubMed  CAS  Google Scholar 

  • Pua E.-C., Lim S.S.-W., Liu P., Liu J.-Z. 2000. Expression of a UDP-glucose pyrophosphorylase cDNA during fruit ripening of banana (Musa acuminata). Aust. J. Plant Physiol. 27: 1151–1159.

    CAS  Google Scholar 

  • Raghothama K.G. 2000. Phosphate transport and signaling. Curr. Opin. Plant Biol. 3: 182–187.

    PubMed  CAS  Google Scholar 

  • Rook F., Corke F., Card R., Munz G., Smith C., Bevan M.W. 2001. Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signalling. Plant J. 26: 421–433.

    Article  PubMed  CAS  Google Scholar 

  • Schiefelbein J.W. 2000. Constructing a plant cell. The genetic control of root hair development. Plant Physiol. 124: 1525–1531.

    Article  PubMed  CAS  Google Scholar 

  • Schmidt W., Schikora A. 2001. Different pathways are involved in phosphate and iron stress-induced alteration of root epidermal cell development. Plant Physiol. 125: 2078–2084.

    Article  PubMed  CAS  Google Scholar 

  • Smeekens S. 2000. Sugar-induced signal transduction in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51: 49–81.

    Article  PubMed  CAS  Google Scholar 

  • Trull M.C., Guiltinan M.J., Lynch J.P., Deikman J. 1997. The responses of wild-type and ABA mutant Arabidopsis thaliana plants to phosphorus starvation. Plant Cell Environ. 20: 85–92.

    Article  CAS  Google Scholar 

  • Yoshida K.T., Fujiwara T., Naito S. 2002. The synergistic effects of sugar and abscisic acid on myo-inositol-1-phosphate synthase expression. Physiol. Plant. 114: 581–587.

    Article  PubMed  CAS  Google Scholar 

  • Wakabayashi K., Sakurai N., Kuraishi S. 1991. Effects of abscisic-acid on the synthesis of cell-wall polysaccharides in segments of etiolated squash hypocotyl. 2. Levels of UDP-neutral sugars. Plant Cell Physiol. 32: 427–432.

    CAS  Google Scholar 

  • Wasaki J., Yonetani R., Kuroda S., Shinano T., Yazaki J., Fujii F., Shimbo K., Yamamoto K., Sakata K., Sasaki T., Kishimoto N., Kikuchi S., Yamagishi M., Osaki M. 2003. Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots. Plant Cell Environ. 26: 1515–1523.

    Article  CAS  Google Scholar 

  • Williamson L.C., Ribrioux S.P.C.P., Fitter A.H., Leyser H.M.O. 2001. Phosphate availability regulates root system architecture in Arabidopsis. Plant Physiol. 126: 875–882.

    Article  PubMed  CAS  Google Scholar 

  • Zhou L., Jang J.C., Jones T.L., Sheen J. 1998. Glucose and ethylene signal transduction crosstalk revealed by an Arabidopsis glucose-insensitive mutant. Proc. Natl. Acad. Sci. USA 95: 10294–10299.

    Article  PubMed  CAS  Google Scholar 

  • Zrenner R., Willmitzer L., Sonnewald U. 1993. Analysis of the expression of potato uridinediphosphate-glucose pyrophosphorylase and its inhibition by antisense RNA. Planta 190: 247–252.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biology, University of Białystok, wierkowa 20b, 15-950, Białystok, Poland

    Iwona Ciereszko

  2. Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden

    Leszek A. Kleczkowski

Authors
  1. Iwona Ciereszko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leszek A. Kleczkowski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Iwona Ciereszko.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ciereszko, I., Kleczkowski, L.A. Phosphate deficiency-dependent upregulation of UDP-glucose pyrophosphorylase genes is insensitive to ABA and ethylene status in Arabidopsis leaves. Acta Physiol Plant 28, 387–393 (2006). https://doi.org/10.1007/BF02706620

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02706620

Key words

Search

Navigation