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Studies on the superoxide anion production and peroxidase activity in potato leaf cell suspension exposed to salicylic acid

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Abstract

It is now widely accepted that salicylic acid (SA) signaling is mediated by reactive oxygen species (ROS) production. We have studied the effect of SA on peroxidase activity and superoxide anion production in potato leaf cell suspension. The results show that potato cells are insensitive to low concentrations of exogenous SA (< 1 mM) and the effect is observed at 1–5 mM SA. The cells exposed to SA exhibit higher peroxidase activity and show different peroxidase pattern when analyzed on native gels compared to the control. Superoxide anion production is enhanced after two hours of treatment and 2.5 mM SA gives the highest value. The results suggest peroxidase-mediated detoxification of ROS elicited by SA.

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Abbreviations

SA:

salicylic acid

SOD:

superoxide dismutase

ROS:

Reactive Oxygen Species

SAR:

Systemic Acquired Resistance

EDTA:

ethylendiaminotetraacetic acid

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Authors and Affiliations

  1. Institute of Biochemistry & Biophysics, University of Tehran, P.O.B: 13145-1384, Tehran, Iran

    Leila Faravardeh & Azra Rabbani

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  1. Leila Faravardeh
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  2. Azra Rabbani
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Correspondence to Azra Rabbani.

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Faravardeh, L., Rabbani, A. Studies on the superoxide anion production and peroxidase activity in potato leaf cell suspension exposed to salicylic acid. Acta Physiol Plant 28, 237–243 (2006). https://doi.org/10.1007/BF02706536

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  • DOI: https://doi.org/10.1007/BF02706536

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