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Jasmonic acid affects changes in the growth and some components content in alga Chlorella vulgaris

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Abstract

The present study was undertaken to test the influence of exogenous applied jasmonic acid upon the growth and changes in some metabolites levels in the cells of green alga Chlorella vulgaris Beijerinck (Chlorophyceae). It was found, that JA in algal cells acted in a concentration-dependent manner. Treatment with JA at high concentrations range of 10−5–10−4 M, resulted in the decrease in cell number and reduction of major photosynthetic pigments, monosaccharides, soluble cellular and extracellular proteins levels as well as decrease in pH of the medium. In contrast to 10−5 and 10−4 M JA, this phytohormone applied at 10−8–10−6 M induced the increase in cell number, photosynthetic pigments and monosaccharides contents, significant accumulation and extracellular secretion of soluble proteins over control and neutralization of the medium. Quantitative changes in polypeptide pattern of total cellular proteins after treatment with the optimal concentration of 10−7 M JA on the 7th day of cultivation as analyzed by SDS-PAGE, was also observed. JA induced synthesis de novo of 15 specific polypeptides with molecular weight 334-16 kDa which were’t detected in the control. The data suggest that JA plays a important role in algal growth and development.

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Abbreviations

JA:

jasmonic acid

MeJA:

methyl jasmonate

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Institute of Biology, University of Białystok, wierkowa 20 B, 15-950, Białystok, Poland

    Romuald Czerpak, Alicja Piotrowska & Katarzyna Szulecka

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  1. Romuald Czerpak
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  2. Alicja Piotrowska
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  3. Katarzyna Szulecka
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Czerpak, R., Piotrowska, A. & Szulecka, K. Jasmonic acid affects changes in the growth and some components content in alga Chlorella vulgaris . Acta Physiol Plant 28, 195–203 (2006). https://doi.org/10.1007/BF02706531

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  • DOI: https://doi.org/10.1007/BF02706531

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