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Bone growth response with porous hydroxyapatite granules in a critical sized lapine tibial-defect model

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This study evaluated the tissue reaction to porous hydroxyapatite (HA) granules in a critical sized tibial-defect of New Zealand white rabbits for a period of 2, 6, 12 and 24 weeks. Physicochemical characterizations of the granules were done using transmission electron microscopy, scanning electron microscopy, X-ray diffraction, and fourier transform infrared spectroscopy to analyse the microstructure, composition, phase purity crystallinity and functional groups of HA. Prior toin vivotesting, the HA granules had proved to be biocompatible and cytocompatibleen route in vitrostudies using L929 mouse fibroblast cells. In the histologic evaluation, as early as 2 weeks, bone ingrowth was observed in the pores and interstices of the granules forming a network of bony trabeculae and over 6, 12 and 24 weeks, it was seen that the granules assisted in bone formation. Fluorochrome multilabels of yellow, red and orange lines showed active sites of bone mineralization in progress in the pores and periphery of the granules. Good osteointegration of the granules with the host bone was observed. There was neither inflammation nor fibrous tissue interposition while resorption of the material was in effect a slow process, since the HA granules still persisted after 24 weeks.

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References

  1. Alper G A, Bernick S, Yazadi M and Nimni M E 1989Am. J. Med. Sci. 298 371

    Article  CAS  Google Scholar 

  2. Baron R, Neff L, Louvard D and Courtoy P J 1985J. Cell Biol. 101 2210

    Article  CAS  Google Scholar 

  3. Blaschke R J, Hewlett A R, Desprez P Y, Peterson O W and Bissell M J 1994Meth. Enzymol. 245 535

    CAS  Google Scholar 

  4. Binderman I and Fin N 1990 inCRC handbook of bioactive biomaterials (eds) T Yamamuro, L L Hench and J Wilson (Boca Raton: CRC Press) p. 45

    Google Scholar 

  5. Buck B E, Malinin T I and Brown M D 1989Clin. Orthop. 240 129

    Google Scholar 

  6. Butts T E, Peterson L J and Allen C M 1989J. Oral Maxillofac. Surg. 47 475

    CAS  Google Scholar 

  7. Chen C S, Mrksich M, Huang S, Whitesides G M and Ingber D E 1997Science 276 1425

    Article  CAS  Google Scholar 

  8. Daculsi G, LeGeros R Z and Mitre D 1989Calcif. Tiss. Int. 45 95

    Article  CAS  Google Scholar 

  9. Daculsi G, LeGeros R Z, Heughebaert J C and Barbieux J 1990Calcif. Tissue Int. 46 20

    Article  CAS  Google Scholar 

  10. Damien C J and Parsons J R 1992J. Appl. Biomater. 2 187

    Article  Google Scholar 

  11. de Bruijn J D, Bovell Y P and van Blitterswijk C A 1994Biomaterials 15 543

    Article  Google Scholar 

  12. de Groot K 1980Biomaterials 1 47

    Article  Google Scholar 

  13. de Groot K (ed.) 1983 inBioceramics of calcium phosphate (Boca Raton: CRC Press) p. 99

    Google Scholar 

  14. de Groot K, Tencer A, Waite P, Nichols J and Kay J 1988 inBioceramics: materials characteristics versus in vivo behaviour (eds) P Ducheyne and J Lemons (New York: Academic Sciences) p. 272

    Google Scholar 

  15. Ducheyne P, El-Ghannam A and Shapiro I A 1994J. Cell Biochem. 56 162

    Article  CAS  Google Scholar 

  16. El-Ghannam A, Ducheyne P and Shapiro I M 1999J. Orthop. Res. 17 340

    Article  CAS  Google Scholar 

  17. Eggli P S, Muller W and Schenk R K 1988Clin. Orthop. & Relat. Res. 232 127

    CAS  Google Scholar 

  18. Finn R A, Bell W H and Brammer J A 1980J. Maxillofac. Surg. 8 211

    Article  Google Scholar 

  19. Flautre B, Descamps M, Delecourt C, Blary M C and Hardouin P 2001J. Mater. Sci. Mater. Med. 12 679

    Article  CAS  Google Scholar 

  20. Glowacki J and Mulliken J B 1985Clin. Plast. Surg. 12 233

    CAS  Google Scholar 

  21. Greiff J 1978Injury 10 257

    Article  Google Scholar 

  22. Grenga T E, Zins J E and Bauer T W 1989Plast. Reconstr. Surg. 84 245

    Article  CAS  Google Scholar 

  23. Gurr E 1973Biological staining methods (Buckinghamshire, England: Searle Diagnostic Gurr Products) 8th ed. p. 111

    Google Scholar 

  24. Hench L L 1991 inThe bone-biomaterial interface (ed.) J E Davies (Canada: Univ. of Toronto Press) p. 33

    Google Scholar 

  25. Hench L L 1994Bioactive ceramics: Theory and clinical applications in bioceramics (eds) O H Anderson and A YliUrpo (Oxford, England: Buterworth-Heinemann Ltd.) pp 3–14

    Google Scholar 

  26. Hench L L and Wilson J 1993 inAn, introduction to bioceramics, advanced series in ceramics (eds) L L Hench and J Wilson (Singapore: World Scientific) 1 p. 1

    Google Scholar 

  27. Holmes R E 1979Plast. Reconstr. Surg. 63 626

    CAS  Article  Google Scholar 

  28. Holmes R E and Hagler H K 1987J. Oral Maxillofac. Surg. 45 421

    CAS  Google Scholar 

  29. Holmes R E, Mooney V, Bucholz R and Tencer A 1984Clin. Orthop. 188 252

    CAS  Google Scholar 

  30. Hoogendoorn H A, Renooij W, Akkermans L M A, Visser W and Wittebol P 1984Clin. Orthop. 187 281

    CAS  Google Scholar 

  31. Hulbert S Fet al 1970J. Biomed. Mater. Res. 4 433

    Article  CAS  Google Scholar 

  32. Hyakuna K, Yamamuro T, Kotoura Y, Kakutani Y, Kitsugi T, Takagi H, Oka M and Kokubo T 1989J. Biomed. Mater. Res. 23 1049

    Article  CAS  Google Scholar 

  33. Ishaug S L, Crane G M, Miller M J, Yasko A W, Yaszemski M J and Mikos A G 1997J. Biomed. Mater. Res. 36 17

    Article  CAS  Google Scholar 

  34. Ishaug-Riley S L, Crane-Kruger G M, Yaszemski M J and Mikos A G 1998Biomaterials 19 1405

    Article  CAS  Google Scholar 

  35. Jarcho M 1981Clin. Orthop. Rel. Res. 157 259

    CAS  Google Scholar 

  36. Jarcho M, Kay J F, Gumaer K I, Doremus R N and Drobeck H P 1977J. Bioeng. 1 79

    CAS  Google Scholar 

  37. Jin Q M, Takita H, Kohgo T, Atsumi K, Itoh H and Kuboki Y 2000J. Biomed. Mater. Res. 51 491

    Article  CAS  Google Scholar 

  38. Klawitter J J and Hulbert S F 1971J. Biomed. Mater. Res. Symp. 2 161

    Article  Google Scholar 

  39. Klein C P, Driessen A A, de Groot K and van den Hoof 1983J. Biomed. Mater. Res. 17 769

    Article  CAS  Google Scholar 

  40. Klein C P, Wolke J G, de Blieck Hogervorst J M and de Groot K 1994J. Biomed. Mater. Res. 28 961

    Article  CAS  Google Scholar 

  41. Kokubo T, Kushitani H, Sakka S, Kitsugi T and Yamamuro T 1990J. Biomed. Mater. Res. 24 721

    Article  CAS  Google Scholar 

  42. Kremer E Aet al 1998J. Bone Miner. Res. 13 1890

    Article  CAS  Google Scholar 

  43. Lebaron R G and Athanasiou K A 2000Tissue Eng. 6 85

    Article  CAS  Google Scholar 

  44. LeGeros R Z 1988Adv. Dent. Res. 2 164

    CAS  Google Scholar 

  45. LeGeros R Z and LeGeros J P 1993An introduction to bioceramics (Advanced series in Ceramics-Vol. 1) (eds) L L Hench and J Wilson (Singapore, New Jersey: World Scientific Publishing Co. Pvt. Ltd.) 9 p. 139

    Google Scholar 

  46. LeGeros R Z, Daculsi G, Orly I and Gregoire M 1991The bone-biomaterial interface (ed.) J E Davies (Canada: University of Toronto Press) p. 76

    Google Scholar 

  47. Lelievre S A, Weaver V M, Nickerson J A, Larabell C A, Bhaumik A, Petersen O W and Bissell M J 1998Proc. Nat. Acad. Sci. USA 95 14711

    Article  CAS  Google Scholar 

  48. Maxian S H, Zawadsky J P and Dunn M G 1993J. Biomed. Mater. Res. 27 717

    Article  CAS  Google Scholar 

  49. Nesbitt S A and Horton M A 1997Science 276 266

    Article  CAS  Google Scholar 

  50. Ohgushi H, Dohi Y, Tamai S and Tabata S 1993J. Biomed. Mater. Res. 27 1401

    Article  CAS  Google Scholar 

  51. Ohgushi H, Okumura M, Yoshikawa M, Inoue K, Senpuku K and Tamai S 1992J. Biomed. Mater. Res. 26 885

    Article  CAS  Google Scholar 

  52. Ohgushi H, Dohi Y, Yoshikawa T, Tamai S, Tabata S, Okunaga K and Shibuya T 1996J. Biomed. Mater. Res. 32 341

    Article  CAS  Google Scholar 

  53. Ono K, Yamamuro T and Nakamura T 1990Biomaterials 11 265

    Article  CAS  Google Scholar 

  54. Oonishi Het al 2000J. Biomed. Mater. Res. 51 37

    Article  CAS  Google Scholar 

  55. Orly I, Gregoire M, Menanteau J and Dard M 1989J. Biomed. Mater. Res. 23 1433

    Article  CAS  Google Scholar 

  56. Paschalis E Pet al 1995J. Biomed. Mater. Res. 29 1499

    Article  CAS  Google Scholar 

  57. Passuti N, Daculsi G and Martin S 1990 inClinical implant materials (eds) G Heimke, U Soltesz and A Lee (Amsterdam: Elsevier) p. 255

    Google Scholar 

  58. Piecuch J Fet al 1983J. Dent. Res. 62 148

    CAS  Google Scholar 

  59. Reddi A H 2000Tissue Eng. 6 351

    Article  CAS  Google Scholar 

  60. Reddi A H and Huggins C B 1973Proc. Soc. Exp. Biol. Med. 143 643

    Google Scholar 

  61. Ricci J L, Blumenthal N C, Spivak J M and Alexander H 1992J. Oral and Maxillofac. Surg. 50 969

    CAS  Article  Google Scholar 

  62. Ripamonti U, Ma S and Reddi A H 1992Matrix 12 202

    CAS  Google Scholar 

  63. Roth H, Muller W and Spiessl B 1984Monatsschr. Zahnmed. 94 222

    CAS  Google Scholar 

  64. Sampath T K and Reddi A H 1984J. Cell Biol. 98 2192

    Article  CAS  Google Scholar 

  65. Schepers E, Ducheyne P, Barbier L and Schepers S 1993Implant Dent. 2 151

    Article  CAS  Google Scholar 

  66. Schmitz J P and Hollinger J O 1986Clin. Orthop. Rel. Res. 205 299

    Google Scholar 

  67. Shimazaki K and Mooney V 1985J. Orthop. Res. 3 301

    Article  CAS  Google Scholar 

  68. Shors E C and Holmes R E 1993An introduction to bioceramics, advanced series in ceramics (eds) L L Hench and J Wilson (Singapore: World Scientific)1 p.181

    Google Scholar 

  69. Urist M R 1980Fundamental and clinical bone physiology (ed.) M R Urist (Philadelphia: Lippincott) p. 331

    Google Scholar 

  70. Urist M R, Lietze A and Dawson E 1984Clin. Orthop. 187 277

    CAS  Google Scholar 

  71. van Blitterswijk C A, Grote J J, Kruypers W, Blok Van Hoek C J G and Deams W T 1985Biomaterials 6 243

    Article  Google Scholar 

  72. Varma H K and Sivakumar R 1996Preparation and characterization of free flowing hydroxyapatite powders, Proceedings of 2nd international symposium on inorganic phosphate materials (Nagoya, Japan: Phosphorous Research Bulletin) Vol.6, pp 35–38

    Google Scholar 

  73. Vogel A I 1980Textbook of quantitative inorganic analysis (London: ELBS) 5th ed.

    Google Scholar 

  74. Wilson J and Low S J 1992J. Appl. Biomater. 3 123

    Article  CAS  Google Scholar 

  75. Wahlig H and Dingeldein E 1986 inProceedings of the sixth european conference on biomaterials, Bologna, Italy p. 196

  76. Winter M, Griss P, de Groot K, Tagai H, Heimke G, van Dijk H J A and Sawai K 1981Biomaterials 2 159

    Article  CAS  Google Scholar 

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Correspondence to Annie John.

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John, A., Abiraman, S., Varma, H.K. et al. Bone growth response with porous hydroxyapatite granules in a critical sized lapine tibial-defect model. Bull Mater Sci 25, 141–154 (2002). https://doi.org/10.1007/BF02706235

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Keywords

  • Hydroxyapatite granules
  • new bone
  • fibroblast cells