Abstract
This study was carried out to compare EPS (Extracellular Polymeric Substances) composition between conventional activated sludge (AS) and activated sludge dosed with clinoptilolite (CAS). Additionally, those were compared with organic removal efficiency in the effluent in conjunction with EPS concentrations. The experiments were conducted at SRT (Solids Retention Time) ranging from 5 to 100 d. For the CAS, proteins were more readily observed for SRT 20 and 100 d compared to that of the AS. Polysaccharide concentration in the sludge was greatly increased for the CAS, but it was significantly diminished when the SRT was extended. The level of EPS concentration observed from the effluent had the same pattern of variation for the two different types of systems. Regardless of type of reactor, the ratio of proteins for sludge versus effluent was independent of SRT, but the ratio of polysaccharides diminished as SRT increased. In the long run, the degree of protein synthesis directly ascribed to concurrent enhancement of SCOD removal efficiency was slightly more in the CAS. It was decided that clinoptilolite added system could be more reliably retrofitted to a conventional activated sludge process.
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References
Andreadakis, A. D., “Physical and chemical properties of activated sludge flocs,”Water Res.,27, 1707 (1993).
Boreo, V. J., Bowers, A. R. and Eckenfelder, Jr. W. W., “Molecular weight distribution of soluble microbial products in biological systems,”Water Sci. Technol.,34, 241 (1996).
Boyd, A. and Chakrabarty, A. M., “Role of alginate lyase in cell detachment ofPseudomonas aeruginosa,”Appl. Environ. Microbiol.,60, 2355 (1994).
Chudoba, P. and Pannier, M., “Use of powdered clay to upgrade activated sludge process,”Env. Tech.,15, 863 (1994).
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F., “Colorimetric method for determination of sugars and related substances,”Anal. Chem.,28, 350 (1956).
Dudman, W. F.,The role of surface polysaccharides in natural environments, Surface Carbohydrates of the Prokaryotic Cell, Shutherland, I. W., eds., Academic Press, London (1977).
Goodwin, J. A. S. and Forster, C. F.,“A further examination into composition for activated sludge surfaces in relation to their settlement characteristics,”Water Res.,19, 527 (1985).
Hariss, R. H. and Mitchell, R.,“Inhibition of the flocculation of bacteria by biopolymers,”Water Res.,9, 993 (1972).
Hartee, E. F.,“Determination of protein: a modification of the lowry method that gives a linear photometric response,”Anal. Biochemi.,48, 422 (1972).
Jahn, A. and Nielsen, P. H.,“Cell biomass and exopolymer composition in sewer biofilms,”Water Sci. Tech.,37, 17(1988).
Kasapgil, I. O., Saillis, P. J. and Anderson, G. K., “Inert COD production in a membrane anaerobic reactor treating brewery wastewater,”Water Res.,34, 3943 (2000).
Kim, C. G., Lee, H. S. and Yoon, T. I.,“Enhanced nitrification by immobilized clinoptilolite in an activated sludge,”Environ. Eng. Res.,8, 49(2003).
Lee, D. H., Kim, S. J. and Moon, H.,“Preparation of a clinoptilolitetypekorean natural zeolite,”Korean J. Chem. Eng.,16, 525 (1999).
Lee, H. S., Park, S. J. and Yoon, T. I.,“Letterto the editor: Comment on influence of different natural zeolite concentration on the anaerobic digestion of piggery waste,”Biore. Tech.,83, 263 (2002).
Park, S. J., Lee, H. S. and Yoon, T. I.,“The evaluation of enhanced nitrification by immobilized biofilm on a clinoptilolite carrier,”Biore. Tech.,82, 183(2002).
Park, J. H., Lee, Y. O. and Park, J. K.,“The evaluation of excess biomass growth in sewers,”Korean J. Chem. Eng.,20, 878 (2003a)
Park, S. J., Kim, C. G., Yoon, T. I. and Kim, D. W.,“Evaluation of increased denitrification in an anoxic activated sludge using zeolite,”Korean J. Chem. Eng.,20, 492 (2003b).
Pirog, T. P., Grinberg, T. A., Malashenko, Y. and Yu, R.,“Isolation of microorganism-producers of enzymes degrading exopolysaccharides fromAcinetobacter sp.,”Mikrobiologiya,33, 550 (1997).
Roberson, E. B. and Firestone, M. K.,“Relationship between desiccation and exopolysaccharide production in a soilPesudomonas sp.,”Appl. Environ. Microbiol.,58, 1284 (1992).
Sponza, D. T.,“Extracellular polymeric substances and physicochemical properties of flocs in steady-and unsteady-state activated sludge systems,”Process Biochem.,37, 983 (2002).
Urbain, V., Block, J. C. and Manem, J.,“Bioflocculation in activated sludge: An analytical approach,”Water Res.,27, 829 (1993).
Yoon, T. I., Lee, H. S. and Kim, C. G.,“Comparison of pilot scale performances between membrane bioreactor and hybrid conventional wastewater treatment system,”J. Mem. Sci.,242, 5 (2004).
Zhang, X., Bishop, P. L. and Kinkle, B. K.,“Comparison of extraction method for quantifying extracellular polymers in biofilms,”Water Sci. Tech.,39, 211 (1999).
Zhang, X. and Bishop, P. L.,“Biodegradability of biofilm extracellular polymeric substances,”Chemosphere,50, 63 (2003).
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Kim, C.G., Lee, C. & Yoon, Ti. Effect of clinoptilolite addition and solids retention time on the extracellular polymeric substances composition and soluble chemical oxygen demands in activated sludge. Korean J. Chem. Eng. 23, 767–772 (2006). https://doi.org/10.1007/BF02705925
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DOI: https://doi.org/10.1007/BF02705925