Journal of Chemical Sciences

, Volume 112, Issue 4, pp 487–496 | Cite as

Quenching of nucleotide-derived radicals bybisbenzimidazole derivative Hoechst-33258 in aqueous solution

Article

Abstract

The pulse radiolysis technique has been employed to investigate the reaction of DNA-minor-groove ligand bisbenzimidazole Hoechst 33258 with pyrimidine and purine nucleotide-derived radicals. Formation of an N-centred Hoechst-33258 radical is observed. Bimolecular rate constants and the yields of Hoechst-33258 radical have been evaluated. While the rate constant for the reaction of pyrimidine-derived radicals with Hoechst-33258 remained the same (1–2) × 109 dm3 mol−1 s−1, the yields of the Hoechst-33258 radical varied from 25% (5′-cytidine monophosphate) to 75% (5′-guanosine monophosphate) under anoxic conditions. The rate constant values for the reaction of purine-derived radicals with Hoechst-33258, under oxic and anoxic conditions, remained the same whereas with pyrimidine-derived radicals, the rate constant value under oxic conditions was about two orders of magnitude lower than under anoxic conditions. The difference in the yields of Hoechst-33258 radical with various nucleotide-derived radicals suggest the formation of different types of radicals and that the reaction mainly occurs by electron transfer from Hoechst-33258 to the nucleotide radicals.

Keywords

Nucleotide-derived radicals Hoechst-33258 pulse radiolysis 

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References

  1. 1.
    SonntagvonC 1987The chemical basis of radiation biology (London: Taylor and Francis)Google Scholar
  2. 2.
    Jain V 1996Indian J. Nucl. Med. 11 8Google Scholar
  3. 3.
    Singh S P, Jayanath V R, Chandan S, Dawarakanath B S, Singh S, Adhikary A and Jain V 1998Indian J. Exp. Biol. 36 375Google Scholar
  4. 4.
    Smith P J and Anderson C O 1984Int. J. Radiat. Biol. 46 331CrossRefGoogle Scholar
  5. 5.
    Young S D and Hill R P 1989Br. J. Cancer 60 715Google Scholar
  6. 6.
    Martin R F and Denison L 1992Int. J. Radiat. Oncol. Biol. Phys. 23 579Google Scholar
  7. 7.
    Adhikary A, Bothe E, Jain V and Sonntag vonC 1997Radiat. Prot. 32 C1Google Scholar
  8. 8.
    Adhikary A, Bothe E, Sonntag vonC and Jain 1997Radiat. Res. 148 493Google Scholar
  9. 9.
    Adhikary A and Jain V 1997Indian J. Biochem. Biophys. 34 409Google Scholar
  10. 10.
    Nabben F J, Karman J P and Loman H 1982Int. J. Radiat. Biol. 42 23CrossRefGoogle Scholar
  11. 11.
    Guha S N, Moorthy P N, Kishore K, Naik D B and Rao K N 1987Proc. Indian Acad. Sci. (Chem. Sci.) 49 261Google Scholar
  12. 12.
    Priyadarsini K I, Naik D B, Moorthy P N and Mittal J P 1991Proc. 7th Tihany Symp. on Radiat. Chem., Hungarian Chem. Soc., Budapest, p. 205Google Scholar
  13. 13.
    Spinks J W T and Woods R J 1990An introduction to radiation chemistry (New York: Wiley) p 243Google Scholar
  14. 14.
    Panajkar M S, Moorthy P N and Shirke N D 1988 Programming a microcomputer for on-line data acquisition and processing in pulse radiolysis experiment. Part I, Kinetic parameters, BARC Report 1410Google Scholar
  15. 15.
    Sonntagvon C 1991The chemistry of free-radical-mediated DNA damage in physical and chemical mechanisms in molecular radiation biology (eds) W A Glass and M N Verma (New York: Plenum) p. 287Google Scholar
  16. 16.
    Sonntag von C 1992Radiat. Phys. Chem. 39 477Google Scholar
  17. 17.
    Deeble D J, Schulz D and Sonntag von C 1986Int. J. Radiat. Biol. 49 915CrossRefGoogle Scholar
  18. 18.
    Breen A P and Murphy J A 1995Free Radical Biol. Med. 18 1033CrossRefGoogle Scholar
  19. 19.
    Anderson R F and Martin R F 19975th International Workshop on Radiation Damage to DNA Techniques, Quantitation and Mechanisms, Bownesson, Windermore, Lake District, UKGoogle Scholar

Copyright information

© Indian Academy of Sciences 2000

Authors and Affiliations

  • H. Mohan
    • 1
  • A. Adhikary
    • 2
  • V. Jain
    • 2
  • J. P. Mittal
    • 1
    • 3
  1. 1.Radiation Chemistry and Chemical Dynamics DivisionBhabha Atomic Research CentreMumbaiIndia
  2. 2.Department of BiocyberneticsInstitute of Nuclear Medicine and Allied SciencesDelhiIndia
  3. 3.Jawaharlal Nehru Centre for Advanced Scientific ResearchBangaloreIndia

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