Abstract
A novel, disposable-bag bioreactor system that uses wave action for mixing and transferring oxygen was evaluated for BHK 21 C13 cell line growth and Aujeszky’s disease virus (ADV) production. Growth kinetics of BHK 21 C13 cells in the wave bioreactor during 3-day period were determined. At the end of the 3-day culture period and cell density of 1.82 × 106 cells ml-1, the reactor was inoculated with 9 ml of gE- Bartha K-61 strain ADV suspension (105.9 TCID50) with multiplicity of infection (MOI) of 0.01. After a 144 h incubation period, 400 ml of ADV harvest was obtained with titre of 107.0 TCID50 ml−1, which corresponds to 40,000 doses of vaccine against AD. In conclusion, the results obtained with the wave bioreactor using BHK 21 C13 cells showed that this system can be considered as suitable for ADV or BHK 21 C13 cell biomass production.
Similar content being viewed by others
Abbreviations
- ADV:
-
Aujeszky’s disease virus
- TCID50 :
-
tissue culture infectious dose 50.
References
BaijotB, Duchene M and Stephenne J 1987 Production of Aujeszky vaccine by the microcarrier technology“From the ampoule to the 500 litre fermentor”;Dev. Biol. Stand. 66 523–530
Cruz H J, Freitas C M, Alves P M, Moreira J L and Carrondo M J T 2000 Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells;Enz. Microb. Technol. 27 43–52
Gaurina Srček V, Čajavec S, Sladić D and Kniewald Z 2004 BHK 21 C13 cells for Aujeszky’s disease virus production using the multiple harvest process;Cytotechnology 45 101–106
Lojkić M, Markuš-Cizelj Lj, Čajavec S, Zidar V and Gojšić S 1992 Investigation on safety of attenuated Aujeszky’s disease vaccine (Ay-Vak Bartha-6) in piglets and sows;Prax. Vet. 40 315–322
Namdev P K and Lio P 2000 Assessing a disposable bioreactor for attachment-dependent cell cultures;Bio. Pharm. (Feb) 1–5
Puentes E, Eiras A, Cancio E, Nores M V, Aquilera A, Seoane R and Regueiro B J 1993 Comparison of the protective efficacy of Aujeszky’s disease (pseudorabies) virus glycoproteins obtained from different sources;Vet. Microbiol. 35 45–59
Singh V 1999 Disposable bioreactor for cell culture using waveinduced agitation;Cytotechnology 30 149–158
Weber W, Weber E, Giesse S and Memmert K 2002 Optimisation of protein expression and establishment of the Wave Bioreactor for Baculovirus/insect cell culture;Cytotechnology 38 77–85
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Slivac, I., Srček, V.G., RadoŠević, K. et al. Aujeszky’s disease virus production in disposable bioreactor. J. Biosci. 31, 363–368 (2006). https://doi.org/10.1007/BF02704109
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02704109