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Neutron protein crystallography in JAERI

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Abstract

Neutron diffraction provides an experimental method of directly locating hydrogen atoms in proteins. After developing an original neutron detector (neutron imaging plate) and a novel practical neutron monochromator (elastically bent perfect Si monochromator), BIX-type diffractometers which were equipped with these tools were efficiently constructed at JRR-3 in Japan Atomic Energy Research Institute (JAERI), Japan and they have finished many protein crystallographic measurements and interesting results have come one after another. At the same time a method of growing large protein single crystals and a database of hydrogen and hydration have also been developed. In the near future, a pulsed neutron diffractometer for biological macromolecules has been proposed at J-PARC in JAERI.

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References

  1. N Niimura,Curr. Opin. Struct. Biol. 9, 602 (1999)

    Article  Google Scholar 

  2. I Tsyba and R Bau,Chemtracts 15, 233 (2002)

    Google Scholar 

  3. N Niimuraet al, Z. Kristallogr. 218, 96 (2003)

    Article  Google Scholar 

  4. N Niimura,Nucl. Instrum. Methods A349, 521 (1994)

    ADS  Google Scholar 

  5. Y K Hagaet al, J. Appl. Cryst. 32, 878 (1999)

    Article  Google Scholar 

  6. Y K Hagaet al, Nucl. Instrum. Methods A487, 504 (2002)

    ADS  Google Scholar 

  7. N Niimuraet al, Physica B213,214, 929 (1995)

    Google Scholar 

  8. I Tanakaet al, J. Appl. Crystallogr. 32, 525 (1999)

    Article  Google Scholar 

  9. I Tanaka,et al, Physica B283, 295 (2000)

    ADS  Google Scholar 

  10. S Fujiwaraet al, Physica B241-243, 207 (1998)

    ADS  Google Scholar 

  11. I Tanakaet al, J. Appl. Crystallogr. 35, 34 (2002)

    Article  Google Scholar 

  12. K Kuriharaet al, J. Synchrotron Rad. 11, 68 (2004)

    Article  Google Scholar 

  13. M Maedaet al, J. Phys. Soc. Jpn. (Suppl.) A70, 403 (2001)

    Google Scholar 

  14. K Kurihara,J. Phys. Soc. Jpn. (Suppl.) A70, 400 (2001)

    Google Scholar 

  15. A Ostermannet al, Biohpys. Chem. 95, 183 (2002)

    Article  Google Scholar 

  16. T Chatakeet al, Appl. Phys. (Suppl.) A74, s1280 (2002)

    ADS  Google Scholar 

  17. T Chatakeet al, Proteins 50, 516 (2003)

    Article  Google Scholar 

  18. T Chatakeet al, Acta Crystallogr. D59, 2306 (2003)

    Google Scholar 

  19. T Chatakeet al, J. Synchrotron Rad. 11, 72 (2004)

    Article  Google Scholar 

  20. M Maedaet al, J. Synchrotron Rad. 11, 41 (2004)

    Article  ADS  Google Scholar 

  21. K Chiba-Kamoshidaet al (to be submitted)

  22. S Araiet al, Acta Crystallogr. D58, 151 (2002)

    Google Scholar 

  23. I Tanakaet al,JAERI-conf 2001-002 488 (2001)

  24. I Tanakaet al,ESS 03-136-M1 321 (2003)

  25. M Araiet al, ibid. 157

  26. I Tanakaet al, J. Neutron Res. (submitted)

  27. N Niimuraet al, Nat. Struct. Biol. 4, 909 (1997)

    Article  Google Scholar 

  28. T Ohharaet al, J. Am. Chem. Soc. 124, 14736 (2002)

    Article  Google Scholar 

  29. G R Desiraju and T Steiner,The weak hydrogen bond (Oxford University Press, NY, 1999)

    Google Scholar 

Download references

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Author notes

  1. I. Tanaka

    Present address: Faculty of Engineering, Ibaraki University, Hitachi, 316-8511, Ibaraki, Japan

Authors and Affiliations

  1. Neutron Science Research Center, Japan Atomic Energy Research Institute (JAERI), Tokai, 319-1195, Ibaraki, Japan

    I. Tanaka

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  1. I. Tanaka
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Tanaka, I. Neutron protein crystallography in JAERI. Pramana - J Phys 63, 99–106 (2004). https://doi.org/10.1007/BF02704056

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