Advertisement

Journal of Biosciences

, Volume 8, Issue 1–2, pp 121–139 | Cite as

Kringle 4 from human plasminogen:1H-nuclear magnetic resonance study of the interactions between ω-amino acid ligands and aromatic residues at the lysine-binding site

  • M. Llinás
  • A. Motta
  • A. De Marco
  • R. A. Laursen
Article

Abstract

The interactions of theω-amino acid ligandsε-aminocaproic acid andp-benzylaminesulphonic acid with the isolated kringle 4 domain from human plasminogen have been investigated by1H-nuclear magnetic resonance spectroscopy at 300 and 600 MHz. Overall, the data indicate that binding either ligand does not cause the kringle to undergo significant conformational changes. When p-benzylaminesulphonic acid is in excess relative to the kringles, progressive exchange-broadening and high field chemical shifts are observed for the proton resonances of the ligand. The largest effect is seen at the amino end of the molecule, which indicates that the — NH 3 + group of the ligand penetrates deeper into the binding site than does the — SO 3 - . Ligand-binding causes signals from the ring-current shifted Leu46 CH 3 δ .δ groups and from a number of aromatic side-chains to shift. Depending on the ligand, the latter include Tyr-II (Tyr50), Tyr-V (an immobile ring), His-II and His-III imidazole groups and the three Trp indole groups present in kringle 4. In particular,p-benzylaminesulphonic acid-binding induces large high field shifts on the Trp-II H6 triplet and the Trp-III (Trp72) H2 singlet. On the other hand,ε-aminocaproic acid bound to kringle 4 exhibits large chemical shifts of its CH2 proton resonances, which indicates that the lysine-binding site is rich in aromatic side chains.

Overhauser experiments centered on thep-benzylaminesulphonic acid H2,6 and H3,5 aromatic transitions as well as on the shifted Trp-II and Trp-III signals reveal efficient cross-relaxation between these two indole side chains and thep-benzylaminesulphonic acid ring. These experiments also show that the side chains from Phe64, Tyr-II (Tyr50), Tyr-IV, and His-II (His31) interact with the ligand. In combination with reported chemical modification experiments that show requirement of Asp57, Arg71 and Trp72 integrity for ligand-binding, our study underscores the relevance of the Cys51-Cys75 loop in defining the kringles’ lysine-binding site. Furthermore, the Cys22-Cys63 loop is folded so as to place His31, His33, Tyr41 and Leu46 in proximity to the binding site. The involvement of residues within the Cys51-Cys75 loop in ligand-binding suggests that Trp-II and Tyr-IV may correspond to Trp62 and Tyr74, respectively. As shown by Overhauser experiments, these two residues are in close contact with each other. From these studies and from the shielding and deshielding effects caused byp-benzylaminesulphonic acid, we suggest that the ligand is sandwiched between the indole rings of Trp-II and Trp-III, which form part of the hydrophobic binding site.

keywords

Kringle 4 kringle structure lysine-binding site nuclear magnetic resonance plasminogen 
BASA

p-benzylaminesulphonic acid

K4

kringle 4

NOE

nuclear Overhauser effect

ppm

parts-per-million

pH*

glass electrode pH reading unconnected for deuterium isotope effects

rf

radio frequency

TSP

sodium 3-trimethylsilyl(2,2,3,3-2H4) propionate

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bothner-by, A. A. (1979) inBiological Applications of Magnetic Resonance, (ed. R. G. Shulman) (New York; Academic Press) p. 177.CrossRefGoogle Scholar
  2. Bovey, F. A. (1969)Nuclear Magnetic Resonance Spectroscopy, (New York: Academic Press) p. 264.Google Scholar
  3. De Marco, A. (1977)J. Magn. Reson.,26, 527.Google Scholar
  4. De Marco, A., Hochschwender, S. M., Laursen, R. A., and Llinás, M. (1982)J. Biol. Chem.,257, 12716.PubMedGoogle Scholar
  5. De Marco, A., Laursen, R. A., and Llinás, M. (1985a)Biochim. Biophys. Acta,827, 369.CrossRefGoogle Scholar
  6. De Marco, A., Pluck, N. D., Bányai, L., Trexler, M., Laursen, R. A., Patthy, L., Llinás, M. and Williams, R. J. P. (1985b)Biochemistry,24, 748.CrossRefGoogle Scholar
  7. De Marco, A., Motta, A., Laursen, R. A. and Llinás, M. (1985c)Biophys. J., (in press).Google Scholar
  8. Ernst, R. E. (1966)Adv. Magn. Reson.,2, 1.CrossRefGoogle Scholar
  9. Ferrige, A. G. and Lindon, J. C. (1978)J. Magn. Reson.,31, 337.Google Scholar
  10. Hochschwender, S. M. and Laursen, R. A. (1981)J. Biol. Chem.,256, 11172.PubMedGoogle Scholar
  11. Hochschwender, S. M., Laursen, R. A., De Marco, A., Llinás, M. (1983)Arch. Biochem. Biophys.,223, 58.CrossRefGoogle Scholar
  12. Lerch, P. G. and Rickli, E. E. (1980)Biochim. Biophys. Acta. 625, 374.CrossRefGoogle Scholar
  13. Lerch, P. G., Rickli, E. E., Lergier, W. and Gillessen, D. (1980)Eur. J. Biochem.,107, 7.CrossRefGoogle Scholar
  14. Llinás, M., De Marco, A., Hochschwender, S. M. and Laursen, R. A. (1983)Eur. J. Biochem.,135, 379.CrossRefGoogle Scholar
  15. Mark wardt, F. (1978) in Fibrinolytics and Antifibrinolytics (ed. F. Markward,) (Berlin: Springer-Verlag) p. 511.CrossRefGoogle Scholar
  16. Okamoto, S., Oshiba, S., Mihara, H. and Okamoto, U. (1968)Ann. N. Y. Acad. Sci.,146, 414.CrossRefGoogle Scholar
  17. Patthy, L., Trexler, M., Váli, Z., Bányai, L. and Varadi, A. (1984)FEBS Lett.,171, 131.CrossRefGoogle Scholar
  18. Perkins, S. J. (1982)Biol. Magn. Resonance,4, 193.CrossRefGoogle Scholar
  19. Sottrup-Jensen, L., Claeys, H., Zajdel, M., Petersen, T. E. and Magnusson, S. (1978)Progr. Chem. Fibrinolysis Trombolysis,3, 191.Google Scholar
  20. Trexler, M., Váli, Z. and Patthy, L. (1982)J. Biol. Chem.,257, 7401.PubMedGoogle Scholar
  21. Trexler, M., Bányai, L., Patthy, L., Pluck, N. D. and Williams, R. J. P. (1983)FEBS Lett.,154, 311.CrossRefGoogle Scholar
  22. Trexler, M. and Patthy, L. (1983)Proc. Natl. Acad. Sci. USA,80, 2457.CrossRefGoogle Scholar
  23. Trexler, M. and Patthy, L. (1984)Biochim. Biophys. Acta,787, 275.CrossRefGoogle Scholar
  24. Winn, E. S., Hu, S. P., Hochschwender, S. M. and Laursen, R. A. (1980)Eur. J. Biochem.,104, 579.CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 1985

Authors and Affiliations

  • M. Llinás
    • 1
  • A. Motta
    • 1
  • A. De Marco
    • 1
  • R. A. Laursen
    • 1
    • 2
  1. 1.Department of ChemistryCarnegie-Mellon UniversityPittsburghUSA
  2. 2.Department of ChemistryBoston UniversityBostonUSA

Personalised recommendations