A critique of the utility of the prediction of protein secondary structure
- 30 Downloads
The Chou-Fasman predictive algorithm for determining the secondary structure of proteins from the primary sequence is reviewed. Many examples of its use are presented which illustrate its wide applicability, such as predicting (a) regions with the potential for conformational change, (b) sequences which are capable of assuming several conformations in different environments, (c) effects of single amino acid mutations, (d) amino acid replacements in synthesis of peptides to bring about a change in conformation, (e) guide to the synthesis of polypeptides with definitive secondary structure,e.g. signal sequences, (f) conformational homologues from varying sequences and (g) the amino acid requirements for amphiphilicα-helical peptides.
KeywordsProtein structure prediction secondary structures structure secondary proteins
rough endoplasmic reticulum
corticotropin releasing factor
Unable to display preview. Download preview PDF.
- Bourgeois, S. and Pfahl, M. (1976)Adv. Prot. Chem.,30, 1.Google Scholar
- Chou, P. Y. and Fasinan, G. D. (1977a)Peptides, Proceedings of the fifth American Peptide Symposium, (eds M. Goodman and J. Meinhofer) (New York: John Wiley and Sons), p. 284.Google Scholar
- Chou, P. Y. and Fasman, G. D. (1978a)Adv. Enzymol., 47, 45.Google Scholar
- Epand, R. M. (1971)Can. J. Bio. Chem.,49, 166.Google Scholar
- Potts, Jr., J. T., Kronenberg, H. M. and Rosenblatt, M. (1982)Adv. Prot. Chem.,35, 323.Google Scholar
- Ptitsyn, O. B. and Finkelstein, A. V. (1980) inProtein Folding, (ed. R. Jaenicke) (Elsevier/North Holland), 101.Google Scholar
- Sternberg, M. J. E., Cohen, F. E., Taylor, W. R. and Feldman, R. J. (1982)Philos. Trans. R. Soc., London Ser. B,239, 177.Google Scholar