Overexpression ofGbERF confers alteration of ethylene-responsive gene expression and enhanced resistance toPseudomonas syringae in transgenic tobacco
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GbERF belongs to the ERF (ethylene responsive factor) family of transcription factors and regulates the GCC-box containing pathogen-related (PR) genes in the ethylene signal transduction pathway. To study the function of GbERF in the process of biotic stress, transgenic tobacco plants expressingGbERF were generated. Overexpression ofGbERF did not change transgenic plant’s phenotype and endogenous ethylene level. However, the expression profile of some ethylene-inducible GCC-box and non-GCC-box containing genes was altered, such asPR1b, PR2, PR3, PR4,Osmotin, CHN50, ACC oxidase and ACC synthase genes. These data indicate that the cotton GbERF could act as a transcriptional activator or repressor to regulate the differential expression of ethylene-inducible genes via GCC and non-GCCcis-elements. Moreover, the constitutive expression ofGbERF in transgenic tobacco enhanced the plant’s resistance toPseudomonas syringae pvtabaci infection. In conclusion,GbERF mediates the expression of a wide array ofPR and ethylene-responsive genes and plays an important role in the plant’s response to biotic stress.
KeywordsConstitutive expression GbERF Nicotiana tabacum Pseudomonas syringae transgenic plant
1-aminocyclopropane carboxylic aicd
ethylene responsive factor
polymerase chain reaction
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- Cao Y, Song F, Goodman R M and Zheng Z 2006 Molecular characterization of four rice genes encoding ethylene-responsive transcriptional factors and their expressions in response to biotic and abiotic stress;J. Plant Physiol. (in press)Google Scholar
- Liu J Z, Li N, Yang S E and Kung S D 1998 Full-length nucleotide sequence of the tobacco cultivar SR1 encoding 1-aminocyclopropane-1-carboxylate synthase;Plant Physiol. 118 1534Google Scholar
- Reichmann J L, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe O J, Samaha R R, Creelman R, Pilgrim M, Broun P, Zhang J Z, Ghandehari D and Sherman B K 2000Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes;Science 290 2105–2110CrossRefGoogle Scholar
- Sambrook J, Fritsch E F and Maniatis T 1989Molecular cloning: A laboratory manual, 2nd edition (New York: Cold-Spring Harbor Laboratory Press)Google Scholar