Abstract
The effects of theophylline (a phosphodiesterase inhibitor) and cAMP on 17α, 20ß-dihydroxy-4-pregnen-3-one-induced germinal vesicle breakdown was investigatedin vitro in catfish (Clarias batrachus) oocytes. Folliculated oocytes incubated with 17α, 20ß-dihydroxy-4-pregnen-3-one at the concentration of 1 μg/ml induced 93.2 ± 2.23% germinal vesicle breakdown. When the oocytes were prestimulated with 17α,20ß-dihydroxy-4-pregnen-3-one for 6 h and then treated with different concentrations of theophylline, there was a significant drop in the frequency of germinal vesicle breakdown at the concentrations 2.0, 1.5 and 1.0 mM. However, theophylline was found to be incapable of inhibiting germinal vesicle breakdown at its lowest concentration (0.5 inM). In the time course study, significant inhibition of germinal vesicle breakdown was recorded when 1 mM theophylline was added up to 30 h of 17α,20ß-dihydroxy-4-pregnen-3-one Stimulation but the inhibitory effect of theophylline gradually (time dependent manner) declined if the stimulatory time of 17α,20ß-dihydroxy-4-pregnen-3-one was increased. A similar inhibition of germinal vesicle breakdown was also recorded with various concentrations of cAMP. Except 0.5 mM, all the higher concentrations of cAMP significantly inhibited 17α,20ß-dihydroxy-4-pregnen-3-one induced germinal vesicle breakdown.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
DeManno D A 1983 Thein vitro effects of phosphodiesterase inhibitors on steroid induced final maturation in yellow perch (Perca flavescens) and brook trout (Salvelinus fontinalis);Am. Zool. 23 937
DeManno D A and Goetz F W 1987a Effects of forskolin, cAMP and cyanoketone on steroid-induced meiotic maturation of yellow perch(Perca flavescens) oocytesin vitro;Gen. Comp. Endocrinol. 66 233–243
DeManno D A and Goetz F W 1987b Steroid-induced final maturation in brook trout (Salvelinus fontinalis) oocytesin vitro: The effects of forskolin and phosphodiesterase inhibitors;Biol. Reprod. 36 1321–1332
Finet B, Jalabert B and Garg S K 1988 Effect of defolliculation and 17α-hydroxy,20ß-dihydro-progesterone on cyclic AMP level in full-grown oocytes of the rainbow trout,Salmo gairdneri;Gamete Res. 19 241–252
Goetz F W 1983 Hormonal control of oocyte final maturation and ovulation in fishes; inFish physiology (eds) W S Hoar, D J Randall and E M Donaldson (New York: Academic Press) Vol. 9B, pp 117–170
Goetz F W and Hennessy T 1984 Thein vitro effects of phosphodiesterase inhibitors on 17α,20ß-dihydroxy-4-pregnen-3-one-induced germinal vesicle breakdown in brook troutSalvelinus Fontinalis oocytes;Comp. Biochem. Physiol. A77 785–786
Haider S and Chaube S K 1995 Changes in total cAMP levels during oocyte maturation in the catfish,Clarias batrachus;Comp. Biochem. Physiol. (in press)
Haider S and Rao N V 1992 Oocyte maturation inClarias batrachus. III. Purification and characterization of maturation-inducing steroid;Fish Physiol. Biochem. 9 505–512
Jalabert B and Finet B 1986 Regulation of oocyte maturation in rainbow trout,Salmo gairdneri: Role of cyclic AMP in the mechanism of action of the maturation inducing steroid (MIS), 17α hydroxy, 20β- dihydroprogesterone;Fish Physiol. Biochem. 2 65–74
Jalabert B, Fostier A, Breton B and Weil C 1991 Oocyte maturation in vertebrates; inVertebrate Endocrinology: Fundamentals and Biomedical Implications (eds) Peter K T Pang and M P Schreibman (New York: Academic Press) Vol. 4A, pp 1–396
Nagahama Y 1987 Endocrine control of oocyte maturation; inHormones and Reproduction in Fishes, Amphibians and Reptiles (eds) D O Norris and R E Jones (New York: Plenum Press) pp 171–202
Rao N V and Haider S 1992 Oocyte maturation inClarias batrachus: In vitro effect of various steroids, salmon gonadotropin, cyanoketone and epostane;Indian J. Exp. Biol. 30 1128–1131
Scott A P and Canario A V M 1987 Status of oocyte maturation-inducing steroids in teleosts; inProceedings of the third international symposium on reproductive physiology offish, Canada (eds) D R Idler, L W Crim and J M Walsh (Marine Science Research Laboratory, St John’s) pp 224–234
Trant J M and Thomas P 1988 Structure-activity relationships of steroids in inducing germinal vesicle breakdown of Atlantic croaker oocytesin vitro;Gen. Comp. Endocrinol. 71 307–317
Upadhyaya N and Haider S 1986 Germinal vesicle breakdown in oocytes of catfish,Mystus vittatus (Bloch): Relativein vitro effectiveness of estradiol-17ß, androgens, corticosteroids, progesterone and other pregnene derivatives;Gen. Comp. Endocrinol. 63 70–76
Zairin M Jr, Asahina K, Furukawa K and Aida K 1992 Plasma steroid hormone profiles during HCG induced ovulation in female walking catfishClarias batrachus;Zool. Sci. 9 607–617
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chaube, S.K., Haider, S. Thein vitro effect of theophylline on 17α, 20ß-dihydroxy-4-pregnen-3-one-induced germinal vesicle breakdown in the catfish,Clarias batrachus . J Biosci 20, 439–444 (1995). https://doi.org/10.1007/BF02703847
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02703847