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Insulin alone can lead to a withdrawal of meiotic arrest in the carp oocyte

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Abstract

Meiotic arrest of oocyte in an Indian carp,Labeo rohita Ham. has been found for the first time to be withdrawn by insulin only. Addition of insulin to oocytesin vitro caused germinal vesicle breakdown (GVBD), one of the first visual markers to determine initiation of the final maturational process. Under the influence of insulin the germinal vesicle (GV) of the oocyte migrated towards the animal pole, reached the micropyle and then dissolved (GVBD). By using different concentrations of insulin i.e., 0.063, 0.63, 6.3 and 12.6 (AM, optimum amount required was found to be 6.3 (AM. Induction of GVBD by insulin could be blocked by cycloheximide (Chx), a translation inhibitor, while actinomycin D (AcD) had no effect suggesting non-involvement of transcriptional activity in this process. Addition of the maturation-inducing steroid 17α,20β-dihydroxy-4-pregnen-3-one (DHP) stimulated (P < 0.01) GVBD of carp oocytes and its combination with insulin showed an additive effect. Gonadotropin (GtH) caused GVBD but its effect was greatly augmented by insulin. Our results demonstrate that not only can insulin alone induce GVBD in carp oocytes, but it also augments the stimulatory effect of DHP or IGF-I or GtH on GVBD. This information will be important in hormonal manipulation during induced breeding of carp.

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Abbreviations

AcD:

Actinomycin D

Chx:

cycloheximide

DHP:

17α,20β-dihydroxy-4-pregnen-3-one

GnRH:

gonadotropin releasing hormone

GtH:

gonadotropic hormone

GV:

germinal vesicle

GVBD:

germinal vesicle breakdown

IGF-I:

insulin-like growth factor-I

MEM:

minimum essential medium

MPF:

maturation promoting factor

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Authors and Affiliations

  1. Central Institute of Freshwater Aquaculture, Kausalyaganga, 751 002, Bhubaneswar, India

    S. Dasgupta, D. Basu, L. Ravi Kumar & S. Bhattacharya

  2. Department of Zoology, School of Life Sciences, Visva-Bharati University, 731 235, Santiniketan, India

    D. Basu & L. Ravi Kumar

  3. Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Jadavpur, 700 032, Kolkata, India

    S. Bhattacharya

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  1. S. Dasgupta
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  2. D. Basu
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  3. L. Ravi Kumar
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  4. S. Bhattacharya
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Correspondence to S. Bhattacharya.

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Dasgupta, S., Basu, D., Kumar, L.R. et al. Insulin alone can lead to a withdrawal of meiotic arrest in the carp oocyte. J Biosci 26, 341–347 (2001). https://doi.org/10.1007/BF02703743

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  • DOI: https://doi.org/10.1007/BF02703743

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