Abstract
Polyadenylation of mRNA is a post-transcriptional phenomenon and is catalysed by both nuclear and cytoplasmic poly(A) polymerases. In rat testis the specific activity of cytoplasmic poly(A) polymerase increased with age reaching a peak value in 80 days. After this time the activity was maintained at a slightly lower level for the next 280 days. Follicle stimulating hormone, luteinizing hormone, human chorionic gonadotropin, pregnant mare serum gonadotropin and a gonadotropin releasing hormone analogue significantly increased the enzyme activity in immature rat testis. These results show that testicular poly(A) polymerase was probably under the influence of gonadotropic hormones.
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Abbreviations
- PMSG:
-
Pregnant mare serum gonadotropin
- hCG:
-
human chorionic gonadotropin
- GnRHa:
-
gonadotropin releasing hormone analogue
- LH:
-
luteinizing hormone
- FSH:
-
follicle stimulating hormone
- DTT:
-
dithiothereitol
- Tris:
-
Tris (hydroxymethyl) methane
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Raju, V.S., Reddy, P.R.K. Hormonal regulation of poly(A) polymerase in the testis of rat. J Biosci 7, 223–227 (1985). https://doi.org/10.1007/BF02703592
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DOI: https://doi.org/10.1007/BF02703592