Abstract
Two moieties of inhibin could be obtained by chromatography of partially purified preparations of inhibin from human placenta on Sephadex G-100, G-25 and ion exchange chromatography on diethylaminoethyl Sephadex A-50. The higher molecular weight moiety (14,000) designated as HPI-H appears to be similar to inhibin from human seminal plasma. While the lower molecular weight moiety (1500) designated as HPI-L appears to be similar to that of sheep testicular inhibin.
The preparations from both human term placenta and human seminal plasma inhibited the binding of [125I] human follicle stimulating hormone to rat testicular receptors. This effect of inhibins could be neutralized by antisera raised against corresponding polypeptide. Further these antibodies could neutralize endogenous inhibin resulting in 2 to 3 fold increase in serum follicle stimulating harmone levels, which could then be reversed by exogenous administration of the isolated inhibin preparations.
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Abbreviations
- HPI-H:
-
Human placental inhibin-high molecular weight
- HPI-L:
-
human placental inhibin-low molecular weight
- FSH:
-
follicle stimulating hormone
- LH:
-
lutinizing hormone
- DEAE:
-
diethylaminoethyl
- PAGE:
-
Polyacrylamide gel electrophoresis
- Con-A:
-
concanavalin A
- NRS:
-
normal rabbit serum
- RIA:
-
radioimmunoassay
- HSPI:
-
human seminal plasma inhibin
- hFSH:
-
human FSH
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