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Induction of ovarian follicular development in the subadult frogRana tigrina using luteinizing hormone releasing hormone-acetate

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Abstract

In the subadultRana tigrina administration of 2 μg luteinizing hormone releasing hormone-acetate/frog six days a week for 4 weeks in April resulted in the formation of medium (in all 8 frogs) and large sized (in 4 out of 8 frogs) yolky oocytes and, concomitant increases in the oviductal mass. The ovarian and oviductal masses showed a 10-fold increase over the control frogs. In untreated frogs the ovaries were transparent and contained first growth phase oocytes only. The oviducts were also infantile.

The pituitary sections were stained using antisera raised in rabbit against the β-subunit of human luteinizing hormone and human follicle stimulating hormone. Immunoreactivity, staining intensity, cytoplasmic granulation and, cell, nuclear and cytoplasmic areas of gonadotrophs (B2 cells) increased significantly in luteinizing hormone releasing hormone treated frogs.

The above findings suggest that pituitary-ovarian axis in the subadultRana tigrina is responsive to luteinizing hormone releasing hormone and that long-term treatment with the hormone induces cytomorphological changes in the gonadotrophs which result in the conversion of inactive cells into secretory cells. This is accompanied by precocious vitellogenic growth of oocytes in the subadult frogs.

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Abbreviations

LHRH:

Luteinizing hormone releasing hormone

GnRH:

gonadotropin releasing hormone

FSH:

follicle stimulating hormone

FGP:

first growth phase

MSGP:

medium sized second growth phase

LSGP:

large sized second growth phase

AF:

atretic folicles

TSH:

thyroid stimulating hormone

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  1. B. Hoque

    Present address: Department of Zoology, University of Chittagong, Chittagong, Bangladesh

Authors and Affiliations

  1. Department of Zoology, Karnatak University, 580 003, Dharwad, India

    B. Hoque, S. K. Saidapur & D. R. Naik

  2. Department of Zoology, Utkal University, 751 004, Bhubaneswar, India

    D. R. Naik

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  1. B. Hoque
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  2. S. K. Saidapur
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  3. D. R. Naik
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Hoque, B., Saidapur, S.K. & Naik, D.R. Induction of ovarian follicular development in the subadult frogRana tigrina using luteinizing hormone releasing hormone-acetate. J. Biosci. 20, 563–572 (1995). https://doi.org/10.1007/BF02703538

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  • DOI: https://doi.org/10.1007/BF02703538

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