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Elevated concentration of glycogen in cobalt induced epileptogenic focus

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Abstract

Changes in the concentration of glycogen in various areas of the brain of epileptic rats were investigated. Epilepsy was induced by implantation of cobalt discs on the right sensory motor cortex and epileptic animals have shown clear tonic-clonic jerks of the contra-lateral fore and hind limbs.

It was found that glycogen concentration was increased by 29% in the epileptogenic sensory motor cortex as compared to the same area in the contra-lateral hemisphere. Glycogen concentration in other areas within the same hemisphere remained unaffected.

Implantation of nickel or copper on the same sensory motor cortex, which did not cause the typical limb jerks of epilepsy, had no effect on glycogen concentration in the same treated areas. Assay of relevant metabolites in the epileptic cortex showed an increase in the concentration of pyruvate and glucose-6-phosphate, by 218% and 112% respectively. The results suggest that the increased glycogen concentration in epileptogenic focus results from increased uptake of glucose due to neuronal hyperexcitability.

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References

  • Abdul-Ghani A S and Nahas N 1990 Effect of afferent brachial stimulation and photic stimulation on glycogen concentration in cortical brain tissue;Neurochem. Int. 16 115–118

    Article  CAS  Google Scholar 

  • Bergmeyer H O 1974Methods of Enzymatic Analysis 2nd edition (New York: Academic Press)

    Google Scholar 

  • Brunner E A, Passonneau J V and Molstad E 1971 The effect of volatile anesthetics on levels of metabolites and on metabolic rate in brain;J. Neurochem. 18 2301–2316

    Article  CAS  Google Scholar 

  • Dodd P R, Bradford H F, Abdul-Ghani A S, Cox DWG and Coutinho-Netto J 1980 Release of amino acids from chronic epileptic and sub-epileptic fociin vivo;Brain Res. 193 505–517

    Article  CAS  Google Scholar 

  • Folbergrova J, Passonneau J V, Lowry O H and Schultz D W 1969 Glycogen, ammonia and related metabolites in the brain during seizures evoked by methionine sulphoximine;J. Neurochem. 16 191–203

    Article  CAS  Google Scholar 

  • Guth L and Watson P K A 1968 Correlated histochemical and quantitative study on cerebral glycogen after brain injury in the rat;Exp. Neurol. 22 590–602

    Article  CAS  Google Scholar 

  • Hindfelt B 1984 Effect of sustained portal systemic shunting on glycolytic and citric acid cycle intermediates in the rat brain;Eur. J. Clin. Invest. 14 334–338

    Article  CAS  Google Scholar 

  • Hevor T K, Aissi E and Delorme P 1990 Correlation between carbohydrate and catecholamine level impairment in methionine sulfoximine epileptogenic rat brain;Neurochem. Res. 15 861–868

    Article  CAS  Google Scholar 

  • Husain K and Ansari R A 1988 Influence of cholinergic and adrenergic blocking drugs on hyperglycemia and brain glycogenolysis in diazinon-treated animals;Can. J. Physiol. Pharmacol. 66 1144–1147

    Article  CAS  Google Scholar 

  • Husain K and Vijayaraghavan R 1989 DEP induced changes in acetylcholinesterase activity and glycogen level in certain brain regions of mice;Indian. J. Physiol. Pharmacol. 33 250–252

    CAS  PubMed  Google Scholar 

  • Johnson Y A and Fusaro R M 1964 An enzymatic method for the quantitative determination of micro quantities of glycogen;Anal. Biochem. 7 189–191

    Article  CAS  Google Scholar 

  • Krivanek J 1958 Changes of brain glycogen in the spreading EEG-depression of LEAO;J. Neurochem. 2 337–343

    Article  CAS  Google Scholar 

  • Lowry O H, Passoneau J V, Hasselberger F X and Schulz D W 1964 Effect of ischemia on known substrates and cofactors of the glycolytic pathway in brain;J. Biol. Chem. 239 18–30

    CAS  PubMed  Google Scholar 

  • Matin M A and Husain K 1987 Changes in cerebral glycogenosis and related enzymes in diazinon treated hyperglycaemic animals;J. Appl. Toxicol. 7 131–134

    Article  CAS  Google Scholar 

  • Matin M A, Husain K and Khan S N 1990 Modification of diazinon-induced changes in carbohydrate metabolism by adrenalectomy in rats;Biochem. Pharmacol. 38 1781–1786

    Article  Google Scholar 

  • Nahas N and Abdul-Ghani A S 1989 Species directed variation and non-uniform distribution of glycogen in mammalian brains during starvation diabetes and anaesthesia;Neurochem. Int. 14 19–24

    Article  CAS  Google Scholar 

  • Nelson S R, Schultz D W, Passonneau J V and Lowry O H 1968 Control of glycogen and limit dextrinin vivo;J. Neurochem. 15 1271–1279

    Article  CAS  Google Scholar 

  • Nowak T S. Bried R L, Lust W D, Passoneau J V 1985 Changes in brain energy metabolism and glycogen synthesis following transient bilateral ischemia in the gerbil;J. Neurochem. 44 487–494

    Article  CAS  Google Scholar 

  • Palladin A W 1954 Der stoffwechsol in Gehirn bei varsehiedenem funktionellem zustand;Wien Klin. Wschr. 66 473–476

    CAS  PubMed  Google Scholar 

  • Swanson R A 1992 Physiologic coupling of glial glycogen metabolism to neuronal activity in brain;Can. J. Physiol. Pharmacol. Suppl. 70 S138–144

    Article  CAS  Google Scholar 

  • Swanson R A, Yu A C. Sharp F R and Chan P H 1989 Regulation of glycogen content in primary astrocyte culture, effect of glucose analogues, phenobarbital and methionine sulfoximine;J. Neurochem. 52 1359–1365

    Article  CAS  Google Scholar 

  • Swanson R A, Morton M M, Sagar S M and Sharp F R 1992 Sensory stimulation induced local cerebral glycogenolysis: Demonstration by autoradiography;Neuroscience 51 451–461

    Article  CAS  Google Scholar 

  • Walanabe J and Passonneau J V 1973 Factors affecting the turnover of cerebral glycogen and limit dextrinin vivo;J. Neurochem. 20 1543–1554

    Article  Google Scholar 

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Authors and Affiliations

  1. Biology and Biochemistry Department, Faculty of Science, Birzeit University, PO Box 14, Birzeit, West Bank, Palestine

    Nabeel Nahas & Abdul-Salam Abdul-Ghani

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  1. Nabeel Nahas
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  2. Abdul-Salam Abdul-Ghani
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Nahas, N., Abdul-Ghani, AS. Elevated concentration of glycogen in cobalt induced epileptogenic focus. J. Biosci. 20, 493–498 (1995). https://doi.org/10.1007/BF02703532

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  • DOI: https://doi.org/10.1007/BF02703532

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