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Expression ofMycobacterium tuberculosis genes inEscherichia coli

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Abstract

TwoEscherichia coli clones expressingMycobacterium tuberculosis antigens were isolated from a gene-bank in the plasmid vector pBR 325. ‘Western blot’ analysis revealed the presence of a unique protein band of molecular weight 68,000 and 38,000, respectively in cellextracts from each clone. The 68,000 dalton antigen was found to be expressed onEscherichia coli outer surface. Plasmid DNA from a third clone could confer leucine independence on two differentleu B mutants ofEscherichia coli but not on mutants in otherleu genes, pointing to the possibility ofgenetic complementation. Thus,Mycobacterium tuberculosis DNA is capable of expression inEscherichia coli.

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Abbreviations

PBS:

Phosphate buffered saline

BSA:

bovine serum albumin

SDS:

sodium dodecyl sulphate

PAGE:

Polyacrylamide gel electrophoresis

Mr′:

molecular weight

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Authors and Affiliations

  1. Tata Research Development and Design Centre, 1, Mangaldas Road, 411001, Pune, India

    Sudha Bhattacharya, Satishn Ranadive & Alok Bhattacharya

Authors
  1. Sudha Bhattacharya
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  2. Satishn Ranadive
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  3. Alok Bhattacharya
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Bhattacharya, S., Ranadive, S. & Bhattacharya, A. Expression ofMycobacterium tuberculosis genes inEscherichia coli . J Biosci 10, 277–281 (1986). https://doi.org/10.1007/BF02703486

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  • DOI: https://doi.org/10.1007/BF02703486

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