Abstract
It was shown that tRNA fromAzotobacter vinelandii grown in the presence of ammonium chloride lacks ribothymidine while that grown in the absence of the ammonium salt contains this modified nucleoside. [32P]-Labelled tRNA from this organism grown in a medium containing the ammonium salt was digested with RNase T1 and the pseudouridinecontaining tetranucleotide, common to all tRNAs was isolated and analysed for the nucleoside replacing the ribothymidine. It was found to be uridine. Cells previously labelled with [32P]-phosphate in the ammonium salt medium were washed and incubated in the ammonium saltfree medium to test whether ribothymidine would be formed upon removal of the ammonium ions. Methylation of the uridine did not take place.
Similar content being viewed by others
Abbreviations
- T:
-
Ribothymidine
- Ψ :
-
pseudouridine
- 2-DTLC:
-
two dimensional thin-layer chromatography
References
Ajitkumar, P. and Cherayil, J. D. (1982)Curr. Sci., 51,970.
Ajitkumar, P. and Cherayil, J. D. (1985a)J. Bacteriol., 162,752.
Ajitkumar, P. and Charayil, J. D. (1985b)Curr. Sci., 54,262.
Brenner, M., Lewis, J. A., Strauss, D. S., de Lorenzo, F., Ames, B. N. (1972)J. Biol. Chem.,247, 4333.
Brill, W. J. (1980)Microbiol. Rev., 44,449.
Brownlee, G. G.(1972) inDetermination of Sequences in RNA (eds T. S. Work and E. Work) (Laboratory Techniques in Biochemistry and Molecular Biology) p. 204.
Buck, M. and Griffiths, E. (1981)Nucleic Acids Res., 9,401.
Buck, M. and Ames, B. N. (1984)Cell, 36,523.
Buu, A., Menichi B. and Heyman, T. (1981)J. Bacteriol.,146, 819.
Chackalaparampil, I. and Cherayil, J. D. (1981)Biochem. Int.,2, 121.
Gauss, D. H. and Sprinzl, M. (1983)Nucleic Acids Res.,11, r1.
Gordon, J. K. and Brill, W. J. (1972)Proc. Natl. Acad. Sci. USA,69, 3501.
Kersten, H., Albani, M., Mannlein, E., Praisler, R., Wurmbach, P. and Nierhauss, K. H. (1981)Eur. J. Biochem., 114,451.
Marcu, K., Marcu, D. and Dudock, B. (1978)Nucleic Acids Res.,5, 1075.
Morris, R. O., Regier, D. A., Olson, R. M. Jr., Struxness, L. A. and Armstrong, D. J. (1981)Biochemistry,20,6012.
Nishimura, S., Harada, F., Narushima, U. and Seno, T. (1967)Biochim. Biophys. Acta, 142,133.
Sanger, F., Brownlee, G. G. (1967)Methods Enzymol., 12A,361.
Shanmugam, K. T. and Hennecke, H. (1979) inRecent Advances in Biological Nitrogen Fixation (ed. N. S. S. Rao (Bombay: Oxford and Inter. Book House) p. 227.
Smith, J. D. (1967)Methods Enzymol.,12A, 350.
Strandberg, G. W. and Wilson, P. W. (1967)Proc. Natl. Acad. Sci. USA.,58, 1404.
Svensson, I., Isaksson, L. and Henningsson, A. (1971)Biochim. Biophys. Acta,238, 331.
Watanabe, K., Oshima, T., Iijima, K., Yamaizumi, Z. and Nishimura, S. (1980)J. Biochem. (Jpn.),87, 1.
Zamir, A., Holley, R. W. and Marquisee, M.(1965)J. Biol. Chem.,240, 1267.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ajitkumar, P., Cherayil, J.D. Ammonium ions prevent methylation of uridine to ribothymidine inAzotobacter vinelandii tRNA. J Biosci 10, 267–276 (1986). https://doi.org/10.1007/BF02703485
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02703485