Abstract
Immunoaffinity purified Sm/RNP antigens from buffalo and goat liver were studied to determine the role of RNA and proteins towards the antigenicity of Sm and RNP antigens. A more direct approach using enzyme-linked immunosorbent assay on nylon beads has been utilized to look into the problem. The effect of enzyme treatment and the role of RNA and protein fractions in influencing antigenicity have been described. RNA seems to be involved in the maintenance of RNP specific polypeptides in suitable conformation so as to keep them in solution. Removal of RNA leads to insolubilization of RNP specific polypeptides. Antibodies to Sm and RNP antigens have been shown to cross react with poly A containing heterogeneous nuclear ribonucleoprotein with no cross reactivity with thymus RNA or DNA.
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Abbreviations
- RNP:
-
Ribonucleoprotein
- snRNP:
-
small nuclear RNP
- hnRNP:
-
heterogeneous nuclear RNP
- SLE:
-
systemic lupus erythematosus
- DEAE:
-
diethylaminoethyl
- PMSF:
-
phenylmethylsulphonyl fluoride
- IgG:
-
immunoglobulin-G
- PBS:
-
phosphate buffer saline
- ELISA:
-
enzyme-linked immunosorbent assay
- SDS:
-
sodium dodecyl sulphate
- PAGE:
-
polyacrylamide gel electrophoresis
- UV:
-
ultraviolet
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Kumar, A., Ali, R. Cross reactivity and enzyme sensitivity of immunoaffinity purified Sm/RNP antigens. J Biosci 10, 235–242 (1986). https://doi.org/10.1007/BF02703481
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DOI: https://doi.org/10.1007/BF02703481