Abstract
Poly A enriched RNA from either liver or oviduct of estradiol-17β treated immature chicks supported [3H]-leucine incorporation into immunoprecipitable riboflavin carrier protein in a dose-dependent manner when translated in the rabbit reticulocyte lysate system. Primary translation product of riboflavin carrier protein had a molecular weight of 38,000 which on incubation with a stripped hepatic microsomal preparation was processed to a product with a size comparable to native riboflavin carrier protein. Poly A enriched RNA from both the liver and the oviduct of estrogen-treated birds stimulated [3H]-leucine incorporation into riboflavin carrier protein and this was 2–3 fold higher during secondary stimulationvis-a-vis primary stimulation with the steroid. Poly A enriched RNA from the liver of progesteronetreated birds during secondary stimulation did not support riboflavin carrier protein synthesis. In contrast, poly A enriched RNA from the oviduct of the birds treated with progesterone during secondary (but not primary) stimulation did exhibit riboflavin carrier protein-mRNA activity which was comparable to that stimulated by estradiol-17β
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Abbreviations
- RCP:
-
Riboflavin carrier protein
- RIA:
-
radioimmunoassay
- poly A+-RNA:
-
poly Aenriched RNA
- EGTA:
-
ethylene glycol-bis (2-aminoethyl ether) N, N′ -tetraacetic acid
- DTT:
-
dithiothreitol
- HEPES:
-
N-2 hydroxy ethyl piperazine-N′ -2-ethane sulphonic acid
- SDS:
-
sodium dodecyl sulphate
- PPO:
-
2,5-diphenyl oxazole
- M r :
-
molecular weight
- PAGE:
-
polyacrylamide gel electrophoresis
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Kumari, B.D., Adiga, P.R. Correlation between riboflavin carrier protein induction and its mRNA activity in estrogen stimulated chicken liver and oviduct. J Biosci 10, 193–202 (1986). https://doi.org/10.1007/BF02703477
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DOI: https://doi.org/10.1007/BF02703477