Journal of Biosciences

, Volume 13, Issue 4, pp 401–408 | Cite as

Keratinization of rat vaginal epithelium IV. Modulation of transglutaminase activity by oestradiol

  • S. Vijayasaradhi
  • P. D. Gupta


Calcium-dependent transglutaminase activity was found to be present in vaginal homogenates from adult cycling rats. Treatment of immature or adult ovariectomized rats with oestradiol (0.1 μg/g body weight) resulted in 1.5–2-fold enhancement in the enzyme activity. Progesterone treatment (0.1 μg/g body weight) decreased the enzyme activity. Analysis of amino acids produced by proteolytic enzyme digestion of insoluble keratins from rat vaginal tissue indicated the presence of γ-glutamyl-ε-⥿sine dipeptide (4 μmol/g protein) in this protein. These results suggest that oestradiol acts on vaginal tissue and induces the activity of transglutaminase. This enzyme catalyses the formation of γ-glutamyl-ε-lysine crosslinks between keratin polypeptides and thus leads to kerartization of the tissue.


Oestradiol keratinization transglutaminase 


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Copyright information

© Indian Academy of Sciences 1988

Authors and Affiliations

  • S. Vijayasaradhi
    • 1
  • P. D. Gupta
    • 1
  1. 1.Centre for Cellular and Molecular BiologyHyderabadIndia

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