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Hormonal modulation of glycogen reserves In the fat body of castor semilooperAchaea janata Linn (Lepidoptera, Noctuidea)

Abstract

Histochemical details of the fat body in the fifth instar larval stage, pupa and adult moth of the castor semilooperAchaea janata were elucidated in detail using light and electron microscopy in conjunction with glycogen storage patterns using polyacrylamide gel electrophoresis. The periodic-acid Schiff staining for glycogen in fat body was maximum in the spinning stage of the larva, when compared to the feeding stage and prepupal stages, and higher in the pupa than in the larva and the adult moth. In insulin injected and juvenile hormone treated fat body, glycogen deposition was more than in glucagon injected tissues. The periodic-acid Schiff stained bands in PAGE had electrophoretic mobility similar to the corresponding protein band numbers, indicating their glycoprotein nature.

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References

  1. Chefurka W 1965 Intermediary metabolism of carbohydrates in insects; inThe physiology of insecta (ed.) M Rockstein (New York: Academic Press) pp 581–667

    Google Scholar 

  2. Collatz K G and Wilps H 1985 The quantitative relationship between life span, food ingestion, egg production, mating and flight-activity of protein-fed blowflyPhormia terraenovae females;Exp. Gerontol. 20347–357

    PubMed  Article  CAS  Google Scholar 

  3. Cruz-Landim C 1983 The larval fat body ofMelipona quadrifasciata anthidioides Lep (Apidae: Meliponinae);Naturalia (Sao Paulo) 8 7–23

    Google Scholar 

  4. Cruz-Landim C 1985 Modificacoes Das Celulas Do Corpo Gorduroso De Rainhas deApis mellifera L (Hymenoptera: Apinae);Cienc. Cult. (Sao Paulo) 37 471–474

    Google Scholar 

  5. De Roberts E D P and De Roberts E M F 1980Cell and molecular biology (Philadelphia: Saunders College)

    Google Scholar 

  6. Elliott R H and Gillott C 1979 An electrophoretic study of proteins of the ovary, fat body and hemolymph in the migratory grass hopperMelanoplus sanguinipes;J. Insect Physiol. 25 405–410

    Article  CAS  Google Scholar 

  7. Fawcett D W 1981The cell (London: WB Saunders)

    Google Scholar 

  8. Gopalakrishna P K 1980Studies on the effect of starvation of fat body reserves in the larvae of Oryctes rhinoceros, M. Phil thesis, University of Kerala, Trivandrum

    Google Scholar 

  9. John A and Muraleedharan D 1989 Biology and morphometrics of castor semilooperAchaea janata Linn (Lepidoptera: Noctuidea);Uttar Pradesh J. Zool 9 48–55

    Google Scholar 

  10. Kilby B A 1963 The biochemistry of the insect fat body;Adv. Insect Physiol. 1 111–174

    CAS  Google Scholar 

  11. Kim H R and Seo E W 1980 Changes of hemolymph protein inPieris rapae L. during cuticle formation and hardening process;Korean J. Zool 23 1–12

    Google Scholar 

  12. Kim H R and Seo E W 1981 A change of haemolymph proteins during metamorphosis ofP. rapae L;Korean J. Entomol 11 33–41

    Google Scholar 

  13. Lenartowicz E, Zaluska H and Niemierko S 1967 Carbohydrates in the wax moth during development;Acta Biochem. Pol. 14 267–275

    CAS  Google Scholar 

  14. Loughton B G and West A S 1965 The development and distribution of hemolymph proteins in Lepidoptera;J. Insect Physiol. 11 919–932

    Article  CAS  Google Scholar 

  15. Nair K S S and George J C 1964 A histological and histochemical study of the larval fat body ofAnthrenus vorax water house (Dermestidae: Coleoptera);J. Insect Physiol. 10 509–517

    Article  CAS  Google Scholar 

  16. Nair K S S and Karnavar G K 1968 A cytological study of changes in the fat body ofTrogoderma granarium during metamorphosis, with special reference to the proteinaceous granules;J. Insect Physiol. 14 1651–1659

    Article  CAS  Google Scholar 

  17. Pearse AGE 1968Histochemistry, theoretical and applied (London: J and A Churchill)

    Google Scholar 

  18. Raabe M (ed.) 1982 Metabolism; inInsect neurohormones (New York: Plenum Press) pp 203–239

    Google Scholar 

  19. Reynolds E S 1963 The use of lead citrate at high pH as an electron opaque stain in electron microscopy;J. Cell Biol. 17 208–212

    PubMed  Article  CAS  Google Scholar 

  20. Siakotos A W 1960 The conjugated plasma protein of the American cockroach 11. changes during moulting and clotting processes;J. Gen. Physiol. 43 1015–1030

    PubMed  Article  CAS  Google Scholar 

  21. Siegert K and Ziegler R 1983 A hormone from the corpora cardiaca controls fat body glycogen Phosphorylase during starvation in tobacco horn worm larvae;Nature (London) 301 526–527

    Article  CAS  Google Scholar 

  22. Simek, Kodrik D 1986 Changes in tissue glycogen and free carbohydrates of hemolymph during the last larval instar and metamorphosis of the silkworm,Bombyx mori (Lepidoptera);Acta Entomol Bohemoslov. 83 92–100

    CAS  Google Scholar 

  23. Sokal R S 1973 Fine structural alterations with age in the fat body of the adult male houseflyMusca domestica;Z. Zellforsch,140 169–175

    Article  Google Scholar 

  24. Spurr A R 1969 A low viscosity resin embedding medium for electron microscopy;J. Ultrastruct. Res. 26 31–43

    PubMed  Article  CAS  Google Scholar 

  25. Stay B and Clark J K 1971 Fluctuation of protein granules in the fat body of the viviparous cockroachDiploptera punctata during the reproductive cycle;Cell Res. 152 193–219

    Google Scholar 

  26. Takacs B 1979 Electrophoresis of proteins in Polyacrylamide. Slab gel; inImmunological methods (eds) I Leflcovits and B Pernis (New York: Academic Press) pp 81–105

    Google Scholar 

  27. Wigglesworth V B 1967 Cytological changes in the fat body ofRhodnius during starvation, feeding and oxygen want;J. Cell Sci. 2 243–256

    PubMed  CAS  Google Scholar 

  28. Walker P A 1965 The structure of the fat body in normal and starved cockroaches as seen with the electron microscope;J. Insect Physiol. 11 1625–1631

    Article  Google Scholar 

  29. Walker P A 1966 An electron microscope study of the fat body of the mothPhilosamia during growth and metamorphosis;J. Insect Physiol. 12 1009–1018

    Article  Google Scholar 

  30. Wilps H and Zöller Th 1988 Origin of ecdysteroids in females of the blow flyPhormia terraenovae and their relation to reproduction of energy metabolism;J. Insect Physiol. 35 709–717

    Article  Google Scholar 

  31. Wilps H and Gade G 1990 Hormonal regulation of carbohydrate metabolism in the blow flyPhormia terraenovae;J. Insect Physiol. 36 441–499

    Article  CAS  Google Scholar 

  32. Wyatt G R 1967 The biochemistry of sugars and polysaccharides in insects;Adv. Insect. Physiol. 4 287–360

    CAS  Article  Google Scholar 

  33. Wyatt G R and Pan M L 1978 Insect plasma proteins;Annu. Rev. Biochem. 47 799–817

    Article  Google Scholar 

  34. Wu Q Y 1983 Metabolism of the fat body in insects;Insect Knowledge 18 92–93

    Google Scholar 

  35. Zacharius R M, Zell B E, Morrison J H and Woodlock J J 1969 Glycoprotein staining following electrophoresis on acrylamide gels;Anal. Biochem. 30 148–152

    PubMed  Article  CAS  Google Scholar 

  36. Ziegler R, Ashida M, Fallon A M, Wimer L T, Wyatt S S and Wyatt G R 1979 Regulation of glycogen Phosphorylase in fat body ofCecropia silkmoth pupae;J. Comp. Physiol. B131 321–332

    Google Scholar 

  37. Ziegler R and Schulz M 1986 Regulation of carbohydrate metabolism during flight inManduca sexta;J. Insect Physiol. 32 997–1001

    Article  CAS  Google Scholar 

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Correspondence to Annie John.

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John, A., Muraleedharan, D. Hormonal modulation of glycogen reserves In the fat body of castor semilooperAchaea janata Linn (Lepidoptera, Noctuidea). J Biosci 18, 261–270 (1993). https://doi.org/10.1007/BF02703123

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Keywords

  • Glycogen
  • fat body
  • glycoproteins
  • Achaec janata