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The role of eucaryotic cell surface glycoconjugates in DNA replication

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Abstract

Multiple forms of ricin have been isolated from castor bean seeds. Two forms, ricin-1 and ricin-2, differ in their isoelectric pI values and toxicity towards IMR-32 cells. Inhibition of IMR-32 DNA polymerase α2 is more pronounced with ricin-1 (65%) than with ricin-2 (10%). Ricin B chain (pI = 5.2) isolated from ricin-1 binds to IMR-32 cell surfaces as well as inhibits DNA polymerase α2 activity when studiedin vitro. The presence of galβ-linked glycoconjugates near the active site of IMR-32 DNA polymerase α2 has been proposed. Replication modulators which bind to the glycose portion of the enzymes involved in the replication system may need a mandatory binding to cell surface glycoconjugates for their activity.

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Authors and Affiliations

  1. Department of Chemistry, Biochemistry, Biophysics and Molecular Biology Program, University of Notre Dame, 46556, Notre Dame, Indiana, USA

    Subhash Basu, Prabir Bhattacharya & Manju Basu

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  1. Subhash Basu
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  2. Prabir Bhattacharya
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  3. Manju Basu
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Basu, S., Bhattacharya, P. & Basu, M. The role of eucaryotic cell surface glycoconjugates in DNA replication. J Biosci 5 (Suppl 1), 157–163 (1983). https://doi.org/10.1007/BF02702986

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