Abstract
Using a high-efficiency DNA cloning vector pJ1–8, a DNA repair geneuvr1 has been self-cloned in bacteriumHaemophilus influenzae. Chimeric plasmid pKuvrl, carrying wild type allele ofuvr1 gene and flanking DNA sequences, specifically complements auvr1 gene mutation in the bacterial chromosome. Auvr1} mutation could be transferred from chromosome byin vivo recombination to pKuvr1 and isolated and designated as plasmid pKuvrl−. Plasmid pKuvrl carries a 11.3 kb chromosomal DNA insert which was scanned for the presence of any other DNA repair genes by a novel method of directed mutagenesis. Preliminary analysis of the 3 new mutants isolated by this method supports the notion that the insert contains more than one gene concerned with ultraviolet radiation-sensitivity.
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Abbreviations
- UV:
-
Ultraviolet radiation
- BHI:
-
brain heart infusion
- NAD:
-
nicotinamide adenine dinucleotide
- AmpR :
-
ampicillin resistant
- CFU:
-
colony forming units
- OD:
-
optical density
- Rd:
-
rough strain, serotype d
References
Beattie, K. L. and Setlow, J. K. (1970)J. Bacteriol.,101, 808.
Gayada, R. C., Yamamoto, L. T. and Markovitz, A. (1976)J. Bacteriol.,127, 1208.
George, M. F. and Notani, N. K. (1981)Indian J. Exp. Biol.,19, 105.
Goodgal, S. H. and Herriott, R. M. (1961)J. Gen. Physiol.,44, 1201.
Hirt, B. (1967)J. Mol. Biol.,26, 365.
Howard-Flanders, P., Boyce, R. P. and Theriot, L. (1966)Genetics,53, 1119.
Joshi, V. P. and Notani, N. K. (1983)J. Biosci.,5, 339.
Joshi, V. P. and Notani, N. K. (1984)Indian J. Exp. Biol.,22, 625.
Kanade, R. P. and Notani, N. K. (1986)Indian J. Exp. Biol.,24, 108.
LeClerc, J. E. and Setlow, J. K. (1973)Nature New Biol.,241, 172.
Marmur, J. (1961)J. Mol. Biol.,3, 208.
McDonell, M. W., Simon, M. N. and Studier, F. W. (1977)J. Mol Biol.,110, 119.
Notani, N. K. (1981)J. Biosci.,3, 431.
Notani, N. K., Joshi, V. R. and Gopal-Ayengar, A. R. (1971)Proc. Symp. ‘Radiation and Radioisotopes for Industrial Microorganisms’, IAEA, Vienna, p. 43.
Notani, N. K. and Kanade, R. P. (1985)Curr. Sci.,54, 832.
Sancar; A. and Rupp, W. D. (1979)Biochem. Biophys. Res. Commun. 90, 123.
Sancar, A. and Rupp, W. D. (1983)Cell,33, 249.
Sedgwick, B., Setlow, J. K., Boling, M. E. and Allison, D. P. (1975)J. Bacterial.,123, 1208.
Setlow, J. K., Boling, M. E., Beattie, K. L. and Kimball, R. F. (1972)J. Mol Biol.,68, 361.
Setlow, J. K., Notani, N. K., McCarthy, D. and Clayton, N-L., (1981)J. Bacteriol.,148, 804.
Vales, L. D., Chase, J. W. and Murphy, J. B. (1980)J. Bacteriol,143, 887;
Ward, J. E. and Lutkenhaus, J. (1985)Cell,42, 941.
Westerveld, A., Hoejmakers, J. H. J., van Duin, M., de Wit, J., Odijk, H., Pastink, A., Wood, R. D. and Bootsma, D. (1984)Nature (London),310, 425.
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Kanade, R.P., Notani, N.K. Cloning and characterization of a DNA repair gene inHaemophilus influenzae . J. Biosci. 12, 115–123 (1987). https://doi.org/10.1007/BF02702962
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DOI: https://doi.org/10.1007/BF02702962