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Rhizobium strains expressing uptake hydrogenase in different host species of cowpea miscellany

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Abstract

Uptake hydrogenase activity in nodules of green gram (Vigna radiata (L.) (Wilczek)), black gram (Vigna mungo (L.) (Hepper)), cowpea (Vigna unguiculata (L.) and cluster bean (Cyamopsis tetragonoloba (L.) (Taub.)), formed with two Hup+ (S24 and CT2014) and one Hup− (M11)Rhizobium strains, was determined at different levels of external H2 in air atmosphere. Nodules of all the 4 host species formed by inoculation with strains S24 and CT2014, showed H2 uptake but not those formed with strain M11. H2 uptake rates were higher in 1 and 2% H2 in air atmosphere (v/v) than at 5 or 10% levels in all the host species. Variations in the relative rates of H2 uptake were observed both, due to host species as well as due toRhizobium strains. However, no host dependent complete repression of the expression of H2 uptake activity was observed in nodules of any of the host species formed with Hup+ strains.

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Abbreviations

YEMA:

Yeast extract mannitol agar

ARA:

acetylene reduction activity

RE:

relative efficiency

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Authors and Affiliations

  1. Department of Microbiology, Haryana Agricultural University, 125 004, Hisar, India

    K. R. Dadarwal, S. S. Sindhu & H. R. Sharma

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  1. K. R. Dadarwal
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  2. S. S. Sindhu
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  3. H. R. Sharma
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Dadarwal, K.R., Sindhu, S.S. & Sharma, H.R. Rhizobium strains expressing uptake hydrogenase in different host species of cowpea miscellany. J. Biosci. 12, 103–109 (1987). https://doi.org/10.1007/BF02702960

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