Abstract
The inhibitory effects of aflatoxin B1 were found to be related to the gram character in procaryotes, used in this study. Ethylene diamine tetra chloroacetic acid (0.05% w/v) or Tween-80 (0.05 % v/v) addition accentuated the aflatoxin B1 growth inhibition inSalmonella typhi andEscherichia coli at different pH values. The inhibition of lipase production was only 5–20 % inPseudomonas fluorescence ca. 25–48% inStaphylococcus aureus andBacillus cereus at different aflatoxin B1 concentrations (4–16μg/ml).However, inhibition of α-amylase induction was complete in1Bacillus megaterium whereas the inhibition was partial inPseudomonas fluorescence (27–40%) at 32μg aflatoxin B1 concentration. An increase in leakage of cell contents and decreased inulin uptake were observed in toxin incubated sheep red blood cell suspension (1 %) with increased aflatoxin B1 concentration
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Abbreviations
- EDTA:
-
Ethylene diamine tetra chloroacetic acid
- SRBC:
-
sheep red blood cell
References
Anand, S. R. (1971)Indian J. Exp, Biol.,10,177.
Arai, T., Ito, T. and Koyama, Y. (1967)J. Bacteriol.,93,59.
Asplin, F. D. and Carnagham, R. B. A. (1961)Vet. Res.,73,1215.
Averner, M. and Klein, H. P. (1963)Biochim. Biophys. Acta,77, 510.
Bernfeld, P. (1955)Methods Enzymol.,1, 149.
Beuchat, L. R. and Lechowich, R. V. (1971)Appl Microbiol.,21, 119.
Black, H. S. and Altschul, A. M. (1965)Biochem. Biophys. Res. Commun.,19,661.
Blount, W. P. (1961)Turkeys,9,52.
Brown, J. M. M. and Abrams, L. (1965)J. Vet. Res.,32,119.
Burmeister, H. R. and Hesseltine, C. W. (1966)Appl. Microbiol.,14,403.
Butler, W. H. and Barnes, J. M. (1963)Br. J. Cancer,17, 699.
Carnaghan, R. B. A. and Allcroft, R. (1962)Vet. Res.,74, 925.
Childs, V. A. and Legator, M. S. (1966)Life Sci.,5,1053.
Clifford, J. I. and Rees, K. R. (1967)Biochem. J.,103,467.
Corcoran, A. C. and Page, I. H. (1939)J. Biol Chem.,121, 601.
Doherty, W. P. and Campbell, T. C. (1972)Res. Commun. Pathol Pharmacol.,3,601.
Gabliks, K. S., Schaeffer, J. W., Friedman, L. and Wogan, G. (1965)J. Bacteriol.,90,720.
Hesseltine, C. W.,Shotwell, O. L., Ellis, J. J. and Stubblefield, R. D. (1966)Bacteriol Rev.,30,795.
Legator, M. S. (1966)Bacteriol. Rev.,30,471.
Leive, L. (1968)J. Biol. Chem.,243,2373.
Lillehoj, E. B. and Ciegler, A. (1968)J. Gen. Microbiol.,54,185.
Lillehoj, E. B. and Ciegler, A. (1970)Can. J. Microbiol.,16,1059.
Nabney, J. and Nesbitt, B. F. (1965)Analyst,90,155.
Nezval, J. and Bosenberg, H. (1970)Arch. Hyg.,154, 143.
Sargeant, K., Sherioan, A., Kelly, J. O. and Carnaghan, R. B. A. (1961)Nature (London),122,1096.
Shotwell, G. L., Hesseltine, C. W., Stubblefield, R. D. and Sorenson, W. G. (1966)Appl Microbiol.,14,425.
Spensley, P. C. (1963)Endeavour,22, 75.
Stark, A. A. (1980)Ann. Rev. Microbiol.,34,235.
Tiwari, R. P., Dham, C. K.. Gupta, L. K., Saini, S. S., Bhalla, T. C. and Vadehra, D. V. (1984)J. Gen. Appl Microbiol.,30,419.
Tiwari, R. P., Dham, C. K., Bhalla, T. C., Saini, S. S. and Vadehra, D. V. (1985)Appl Environ. Microbiol.,49,904.
Vadehra, D. V. and Harmon, L. G. (1969)J. Appl Bacteriol.,32,147.
Vosdingh, R. A. and Nefe, M. J. C. (1974)Toxicology,2, 107.
Wragg, I. B., Rose, V. C. and Legator, M. S. (1967)Proc. Soc. Exp. Biol. Med.,125,1052.
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Tiwari, R.P., Bhalla, T.C., Saini, S.S. et al. Mechanism of action of aflatoxin B1 . J. Biosci. 10, 145–151 (1986). https://doi.org/10.1007/BF02702849
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DOI: https://doi.org/10.1007/BF02702849