Abstract
The observations that liveMycobacterium leprae after entry into cultured peritoneal macrophages from mice, reduced the EA rosetting macrophages, have been exploited to determine the minimum inhibitory concentration of diamino diphenyl sulphone and rifampicin. Diamino diphenyl sulphone showed a minimum inhibitory concentration of 0.028 μg/ml and rifampicin 0.11μg/ml when given externally. However, there was accumulation of diamino diphenyl sulphone inside the macrophages. At an external concentration of 0.028 μg/ml the concentration inside the macrophage was 0.5μg/ml. The minimum inhibitory concentration for diamino diphenyl sulphone in this assay system is higher by several folds and that for rifampicin is slightly lower, than what is reported earlier with mice foot pad experiments. The minimum inhibitory concentration reported in this assay system is quite close to what is observed forin vitro inhibition ofMycobacterium lufu with both the drugs
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Abbreviations
- DDS:
-
Diamino diphenyl sulphone
- MIC:
-
minimum inhibitory concentration
- SRBC:
-
sheep red blood cells
References
Ambrose, E. J., Khanolkar, S. R. and Chulawalla, R. G. (1978)Lepr. India,50, 131.
Birdi, T. J., Mistry, N. F., Mahadevan, P. R. and Antia, N. H. (1983)Infect. Immun.,41, 121.
Birdi, T. J. and Antia, N. H. (1984)IRCSMed. Sci.,12,256.
Ellard, G. A. and Gammon, P. T. (1969)Int. J. Lepr.,37,398.
Ellard, G. A., Gammon, P. T., Rees, R. J. W. and Waters, M. F. R. (1971)Lepr. Rev.,42, 101.
Ellard, G. A. (1980)Lepr. Rev.,51, 199.
Gordon, G. R., Ghoul, D. C., Murray, J. F., Peters, J. H. and Levy, L. (1974)Int. J. Lepr.,42, 373.
Holmes, I. B. and Hilson, G. R. F. (1972)J. Med. Microbiol.,5,251.
Jagannathan, R. and Mahadevan, P. R. (1985)IRCSMed. Sci.,13,265.
Levy, L. and Peters, J. W. (1976)Antimicrob. Agents Chemother.,9,102.
Mankar, M. V., Birdi, T. J., Mahadevan, P. R. and Antia, N. H. (1984)Proc. Int, Lep. Congress, New Delhi, X/426A.
Mankar, M. V., Jagannathan, R. and Mahadevan, P. R. (1984)J. Biosci.,6,709.
Modderman, Ellen, S. M., Merkins Frans, W. H., ZindemaJan., Hilbers HansW. and Warndroff Titia. (1983)Int. J. Lepr.,51, 191.
Nath, I., Prasad, H. K., Satish, M., Sreevatsa, Dinkar, K. V., Seshadri, P. S. and Iyer, C. G. S. (1982)Antimicrob. Agent Chemother.,21,26.
Ozawa, T., Shepard, C. C. and Karat, A. B. A. (1971)Am. J. Trop. Med. Hyg.,20,274.
Peters, J. H., Gordon, G. R., Murray, J. F., Fieldsteel, A. R., Levy, L. (1975)Antimicrob.AgentsChemother.,8, 551.
Peters, J. H., Murray, J. F., Gordon, G. R. and Gelber, R. H. (1981)Pharmacology,21,162.
Satish, M. and Nath, I. (1981)Int. J. Lepr.,49, 187.
Seydel, J. K. and Ellen Gertrud Wempe (1982)Int. J. Lepr.,50,20.
Seydel, J. K., Wempe, E. G. and Rosenfield, M. (1983)Chemotherapy,29,249.
Shepard, C. C. (1967)Int. J. Lepr.,35, 616.
WHO (1982) WHO technical report series 675.
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Jagannathan, R., Mahadevan, P.R. Minimum inhibitory concentration of drugs againstMycobacterium leprae as determined by anin vitro assay. J. Biosci. 10, 137–144 (1986). https://doi.org/10.1007/BF02702848
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DOI: https://doi.org/10.1007/BF02702848