Abstract
Macroconidia ofMicrosporum canis, when placed in a nutrient medium produce germ tubes within 4–6 h. Precursor incorporation studies showed that protein synthesis occurred prior to RNA synthesis. Sucrose density gradient analysis of wet and dry spore extracts revealed the presence of 16 % and 11 % polysomes respectively. The polysomal content increased to about 50% within 15 min of germination. Synthesis of RNA occurred only after 2 h of germination. Pool equilibration of the radioactive precursors was not limiting to these measurements. Polyadenylated RNA was isolated from macroconidia and was found to comprise 2–2.5 % of the total RNA. The poly(A)+ RNAs were heterodisperse and translatable in a wheat germ cell free translating system. It was concluded that macroconidia ofMicrosporum canis contain pre-formed mRNA which is translated early in germination
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Abbreviations
- [14C]-APH:
-
[14C]-Algal protein hydrolysate
- TCA:
-
trichloroacetic acid
- UV:
-
ultra-violet
References
Aviv, H. and Leder, P. (1972)Proc. Natl. Acad. Sci. USA,69, 1408.
Banumathi, N. and Ramananda Rao, G. (1985)Microbios Lett.,29, 31.
Beilby, J. P. and Kidby, D. K. (1982)Can. J. Microbiol.,28, 623.
Bonnen, A. and Brambl, R. (1983)Exp. Mycol.,7, 197.
Brambl, R. M. (1975)J. Bacteriol.,122,1394.
Cochrane, J. C., Rado, T. A. and Cochrane, V. W. (1971)J. Gen. Microbiol.,65,45.
Erickson, A. H. and Blobel, G. (1983)Methods Enzymol.,96, 38.
Grange, F. and Turian, G. (1981)FEMS Microbiol. Lett.,12, 283.
Jayaram, B. M. and Ramananda Rao, G. (1979)Arch. Microbiol.,120, 67.
Johnson, S. A., Lovett, J. S. and Wilt, F. H. (1977)Dev. Biol.,56, 329.
Leaver, C. J. and Lovett, J. S. (1974)Cell Differ.,3,165.
Lin, F. K., Davies, F. L., Tripathi, R. K., Raghu, K. and Gottlieb, D. (1971)Phytopathology,61, 645.
Linz, J. E. and Orlowski, M. (1982)J. Bacteriol.,150, 1138.
Lovett, J. S. (1975)Bacteriol. Rev.,39,345.
Mirkes, P, E. (1974)J. Bacteriol.,117, 196.
Orlowski, M. and Sypherd, P. S. (1978)Arch. Microbiol.,119,145.
Perry, R. P., La Torre, J., Kelley, D. E. and Greenberg, J. R. (1972)Biochim. Biophys. Acta,262, 220.
Preobrazhensky, A. A. and Spirin, A. S. (1978)Prog. Nucleic Acid Res. Mol Biol.,21, 1.
Randerath, K. and Randerath, E. (1967)Methods Enzymol.,12,323.
Roheim, J. R., Knight, R. H. and Van Etten, J. L. (1974)Dev. Biol.,41, 137.
Scherrer, K. (1980)‘Eukaryotic gene Regulations’ (ed. G. M. Kolodny) (Florida: CRC Press) Vol. 1, p. 57.
Spirin, A. S. (1969)Eur. J. Biochem.,10,20.
Tripathi, R. K. and Gottlieb, D. (1974)Mycologia,66,413.
Van Etten, J. L., Bulla, L. A. and St. Julian, G. (1974)J. Bacteriol.,117, 882.
Van Etten, J. L., Dunkle, L. D. and Knight, R. H. (1976) inThe Fungus Spore (eds D. J. Weber, and W. M. Hess) (New York, London, Sydney, Toronto: John Wiley and Sons) p. 243.
Van Etten, J. L. and Rawn, C. D. (1979)Can. J. Microbiol.,25,375.
Vijaya Manohar, Sirsi, M. and Rarnananda Rao, G. (1977)Arogya-J. Health Sci.,3, 31.
Yagura, T. and Iwabuchi, M. (1976)Exp, Cell Res.,100, 79.
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Banumathi, N., Jayaram, B.M. & Rao, G.R. Preformed messengers inMicrosporum canis macroconidia. J. Biosci. 10, 117–126 (1986). https://doi.org/10.1007/BF02702846
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DOI: https://doi.org/10.1007/BF02702846