Abstract
Mice immunized against DS5-hCG-Β and DS6-hCG-Β, chemical analogs of Β-subunit of human choriogonadotropin (hCG-Β) in which 5 and 6 disulphide bonds respectively were reduced and alkylated, were found to produce antibodies specific to hCG without significant crossreactivity with human lutropin (hLH) as tested in a radioimmunoassay. In contrast, mice immunized against the native hCG-Β subunit produced hLH crossreacting antibodies. While the anti-DS5, DS6-hCG-Β serum was capable of selectively blocking the binding of [125I]-hCG to rat testicular LH/hCG receptors, it failed to inhibit the binding of [125I]-hLH to the same receptors. The radioimmunoassay for hCG using the mouse anti-DS5, DS6-hCG-Β serum was not as sensitive as that employing rabbit anti-DS5, DS6-hCG-Β serum. The minimal detection limit was 5 ng/ml for the mouse antibody as compared to 1 ng/ml for the rabbit antibody.
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Abbreviations
- hCG:
-
human choriogondotropin
- hCG-α and hCG-Β:
-
α and Β subunits of hCG
- hLH:
-
human lutropin
- hFSH:
-
human follitropin
- hTSH:
-
human thyrotropin
- hPRL:
-
human prolactin
- DS5hCG-Β-hem and DS6hCG-Β-hem:
-
reduced and S-alkylated hCG-Β in which 5 and 6 disulphide bonds were modified respectively and then coupled with hemocynanin
- RIA:
-
radioimmunoassay
- CMC:
-
carboxymethyl cysteine
- TC buffer:
-
0.05 M Tris-HCl buffer pH 7.2 containing 1 mM CaCl2
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Supported by U.S. Public Health Service Grant HD 08766 to OPB.
An erratum to this article is available at https://doi.org/10.1007/BF02704649.
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Muralidhar, K., Bhal, O.P. Production of antibodies specific to human chorionic gonadotropin in mice immunized against its chemical analogs. J. Biosci. 4, 369–376 (1982). https://doi.org/10.1007/BF02702749
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DOI: https://doi.org/10.1007/BF02702749